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pGL2-Mcl-1 mut

Plasmid

#19133

Depositor

Wafik El-Deiry

Publication

Ricci et al Cancer Cell. 2007 Jul . 12(1):66-80.

Insert

Mcl-1 promoter (MCL1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

NFkB binding-site mutant, the sequence GGGGTCTTCC…

Available since

Oct. 2, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pRL K-ras 3'UTR mutant

Plasmid

#14805

Depositor

Tyler Jacks

Publication

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

K-ras 3'UTR (Kras Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

let-7 miRNA binding site mutations

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pST_140_LVL2 cam

Plasmid

#179334

Purpose

NT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).

Depositor

Anke Becker

Publication

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout

Use

CRISPR

Mutation

Cas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-EF1a-FLEX(frt)-GCaMP6f-WPRE

Plasmid

#118273

Purpose

pAAV vector for flippase dependent GCaMP6f expression under the control of EF1a promoter

Depositor

Naoshige Uchida

Publication

Menegas et al Nat Neurosci. 2018 Sep 3. pii: 10.1038/s

Insert

GCaMP6f

Use

AAV

Tags

6xHis (N terminal on insert), T7 epitope, and Xpr…

Expression

Mammalian

Promoter

EF1a

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pGLTR-X-GFP

Plasmid

#58245

Purpose

GATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and eGFP

Depositor

Stephan Geley

Publication

Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

Inserts

attR1-Cm(R)-CcdB-attR2
TetR-NLS-T2A-GFP

Use

Lentiviral and RNAi; Gateway destionation vector

Tags

GFP, NLS, T2A, and TetR

Expression

Mammalian

Promoter

SFFV and no

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pWZLblasti-HA-ERK2 GOF

Plasmid

#53174

Purpose

Expresses HA tagged human ERK2 R67SD321N from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Publication

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Human, Mustard Weed)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

R67S/D321N

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
proE-cad670-Luc-mEbox

Plasmid

#42084

Depositor

Kumiko Ui-Tei

Publication

Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

Insert

E-cadherin (CDH1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

contains E-cadherin promoter region from -670 to …

Promoter

E-cadherin

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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proE-cad178-Luc-mEbox

Plasmid

#42082

Depositor

Kumiko Ui-Tei

Publication

Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

Insert

E-cadherin (CDH1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

contains E-cadherin promoter region from -178 to …

Promoter

E-cadherin

Available since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCDNA3 Flag Jnk1a1MKK7B2

Plasmid

#19731

Depositor

Roger Davis

Publication

Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

Insert

Jnk1a1MKK7b2 (Map2k7, MAPK8 Mouse, Human)

Tags

Flag

Expression

Mammalian

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
FLAG-Foxo1-ADA 6KQ

Plasmid

#17563

Depositor

Domenico Accili

Publication

Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

Insert

Foxo1 ADA 6KQ (Foxo1 Mouse)

Tags

Flag and Myc

Expression

Mammalian

Mutation

K242, K245, K259, K262, K271 and K291 were replac…

Promoter

CMV

Available since

Oct. 20, 2008

Availability

Academic Institutions and Nonprofits only
pM2AH + mSlo1

Plasmid

#16152

Depositor

Larry Salkoff

Publication

Butler et al Science. 1993 Jul 9. 261(5118):221-4.

Insert

Mus musculus potassium large conductance calcium-activated channel (Kcnma1 Mouse)

Expression

Mammalian

Mutation

N/A

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
cytosolic moPrP (3F4)

Plasmid

#1321

Depositor

Susan Lindquist

Publication

Ma et al Proc Natl Acad Sci U S A 2001 Dec 18;98(

Insert

PrP (Prnp Mouse)

Expression

Mammalian

Mutation

N-term truncation (to make gene cytosolic), vario…

Available since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
FgH1tUTCherry

Plasmid

#85552

Purpose

Inducible expression of guide with fluorescent Cherry reporter

Depositor

Marco Herold

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

H1t

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pOpen-RNAse A

Plasmid

#165565

Purpose

Ribonuclease A or RNase A; endoribonuclease purified from bovine pancreas. Important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, ribonuclease protection assays, mapping single-base mutations in DNA/RNA.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

RNAse A

Use

Synthetic Biology

Available since

Aug. 17, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pOpen-T7gene1

Plasmid

#165505

Purpose

Highly processive DNA-dependent RNA polymerase derived from T7 bacteriophage

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

T7 RNA Polymerase

Use

Synthetic Biology

Available since

July 27, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCL25

Plasmid

#15456

Depositor

Chia Lee

Publication

Luong et al J Microbiol Methods. 2007 Jul . 70(1):18

Type

Empty backbone

Use

S. aureus-e. coli shuttle vector

Available since

Oct. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pOpen-Tthlig

Plasmid

#165569

Purpose

Taq DNA Ligase

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

Tth DNA Ligase

Use

Synthetic Biology

Available since

Oct. 25, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pOpen-SP6rpol

Plasmid

#165516

Purpose

DNA-dependent RNA polymerase derived from SP6 bacteriophage that catalyzes the transcription of viral genes.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

SP6 RNA Polymerase

Use

Synthetic Biology

Available since

July 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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PSL1180polyUBdsRED

Plasmid

#49327

Purpose

Donor plasmid for homologous recombination that expresses dsRED constitutively using the Aedes aegypti polyubiquitin (PUb) promoter.

Depositor

Leslie Vosshall

Insert

dsRED

Expression

Insect

Promoter

Aedes polyubiquitin

Available since

Nov. 18, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pXP318

Plasmid

#26837

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Publication

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Expression

Yeast

Available since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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