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pS23.U6<ActB.mus_C-term-KI_gRNA

Plasmid

#207408

Purpose

gRNA from a U6 promoter targeting the mouse ActB near the 3' end of the ORF. Used for C-terminal knockin by DSB repair. [Lab plasmid ID: TU260]

Depositor

Alexandros Poulopoulos

Article

Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

Insert

ActB

Use

CRISPR and Mouse Targeting

Promoter

U6

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Intersectin I SH3 B domain (human)

Plasmid

#47414

Depositor

Peter McPherson

Article

Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

Insert

Intersectin SH3 domain B (ITSN1 Human)

Tags

GST

Expression

Bacterial

Mutation

only SH3 domain B

Promoter

tac

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
VRK3_HUMAN_D0

Plasmid

#79681

Purpose

This plasmid encodes the kinase domain of VRK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

VRK3 (VRK3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pBS/U6/RNAi gamma-tubulin shRNA mouse

Plasmid

#70666

Purpose

Reduced the expression of gamma-tubulin in murine cells

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

5´-GGCTCATGATGGCCAACCACCAAGCTTGGTGGTTGGCCATCATGAGCCCTTTTTC-3´ 5´-CCGAGTACTACCGGTTGGTGGTTCGAACCACCAACCGGTAGTACTCGGGAAAAAGTTAA-3´ (Tubg1, Tubg2 Mouse)

Use

U6 promoter

Expression

Mammalian

Promoter

U6

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pSL0721 (pDonor_L1-105)

Plasmid

#130645

Purpose

Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened left end. Total transposon size = 933 bp.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchCAST donor DNA (short L)

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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PHKG2_HUMAN_D0

Plasmid

#79736

Purpose

This plasmid encodes the kinase domain of PHKG2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

PHKG2 (PHKG2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
VRK2_HUMAN_D0

Plasmid

#79737

Purpose

This plasmid encodes the kinase domain of VRK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

VRK2 (VRK2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pSL0715 (pDonor_R1-57)

Plasmid

#130644

Purpose

Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened right end. Total transposon size = 910 bp.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchCAST donor DNA (short R)

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCDB181

Plasmid

#80692

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEHEE_06

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gEHEE_06

Tags

OsmY-10His-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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Intersectin I SH3 C domain (human)

Plasmid

#47415

Depositor

Peter McPherson

Article

Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

Insert

Intersectin SH3 domain C (ITSN1 Human)

Tags

GST

Expression

Bacterial

Mutation

only SH3 domain C

Promoter

tac

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pCDH-AtAFB2-mTagBFP2

Plasmid

#179889

Purpose

Expresses AtAFB2 (auxin-inducible degron system) fused to mTagBFP2 (lentiviral plasmid)

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

AtAFB2 (AFB2 Mustard Weed, Synthetic)

Use

Lentiviral

Tags

mTagBFP2

Expression

Bacterial and Mammalian

Promoter

EF1

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pEMS2131

Plasmid

#111895

Purpose

ssAAV genome with MCS for inserting MiniP promoter to drive an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Type

Empty backbone

Use

AAV

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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Intersectin I SH3 D domain (human)

Plasmid

#47416

Depositor

Peter McPherson

Article

Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

Insert

Intersectin SH3 domain D (ITSN1 Human)

Tags

GST

Expression

Bacterial

Mutation

only SH3 domain D

Promoter

tac

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
Intersectin I SH3 E domain (human)

Plasmid

#47417

Depositor

Peter McPherson

Article

Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

Insert

ITSN1 intersectin 1 (ITSN1 Human)

Tags

GST

Expression

Bacterial

Mutation

only SH3 domain E

Promoter

tac

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/GS-DJ1-E64D-V5

Plasmid

#29344

Depositor

Mark Cookson

Article

Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

Insert

DJ1 (PARK7 Human)

Tags

His6 and V5 epitope

Expression

Mammalian

Mutation

Glu64Asp

Available Since

April 20, 2011

Availability

Academic Institutions and Nonprofits only
PMYT1_HUMAN_D0

Plasmid

#79687

Purpose

This plasmid encodes the kinase domain of PMYT1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

PMYT1

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/GS-DJ1-D149A-V5

Plasmid

#29345

Depositor

Mark Cookson

Article

Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

Insert

DJ1 (PARK7 Human)

Tags

His6 and V5 epitope

Expression

Mammalian

Mutation

Asp149Ala

Available Since

April 20, 2011

Availability

Academic Institutions and Nonprofits only
EPHB4-gRNA1/2

Plasmid

#139974

Purpose

gRNA used for knockin EGFP into EPHB4 allele

Depositor

James Thomson

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

Insert

EPHB4 gRNA

Expression

Mammalian

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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EFNB2-gRNA3/4

Plasmid

#139973

Purpose

gRNA used for knockin tdTomado into EFNB2 allele

Depositor

James Thomson

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jul 25;11

Insert

EFNB2-gRNA

Expression

Mammalian

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pWJPrP1

Plasmid

#22109

Depositor

Susan Lindquist

Article

Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

Insert

mouse PrP ORF insert (Prnp Mouse)

Use

Construction of targeting vector

Mutation

mutations at aa 108 and 111 to make 3F4 epitope (…

Available Since

Oct. 16, 2009

Availability

Academic Institutions and Nonprofits only
CSK21_HUMAN_D0

Plasmid

#79707

Purpose

This plasmid encodes the kinase domain of CSK21. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

CSK21

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pCDB267

Plasmid

#80695

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gHHH_06

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gHHH_06

Tags

OsmY-10His-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDB278

Plasmid

#80694

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gHH_44

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gHH_44

Tags

OsmY-10His-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCDB294

Plasmid

#80693

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gHEEE_02

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gHEEE_02

Tags

OsmY-10His-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDB199

Plasmid

#80691

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEHE_06

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gEHE_06

Tags

OsmY-10His-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDB289

Plasmid

#80690

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEEHE_02

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gEEHE_02

Tags

OsmY-10His-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDB185

Plasmid

#80688

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEEH_04

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gEEH_04

Tags

OsmY-10His-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDB285

Plasmid

#80687

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEEEH_04

Depositor

David Baker

Article

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gEEEH_04

Tags

OsmY-10His-Smt3

Expression

Bacterial

Promoter

T7

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6P1‐Afu rnhB

Plasmid

#108694

Purpose

For expression in E coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII

Depositor

Andrew Jackson, Martin Reijns

Article

Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

Insert

rnhB (AF_RS03165 Archaeoglobus fulgidus)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
M26I DJ-1 TEV site pET15b

Plasmid

#60685

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

M26I DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

M26I and TEV site was inserted (see comments belo…

Promoter

T7

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
KAPCA_HUMAN_D0

Plasmid

#79741

Purpose

This plasmid encodes the kinase domain of KAPCA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KAPCA

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
M26L DJ-1 TEV site pET15b

Plasmid

#60686

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

M26L DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

M26L and TEV site was inserted (see comments belo…

Promoter

T7

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
p47 PX(1-121)/p40phox(136-339)

Plasmid

#19018

Depositor

Michael Yaffe

Article

Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19

Insert

p40phox/p47phox (NCF4, NCF1 Human)

Tags

His

Expression

Bacterial

Mutation

insert containing amino acids 1-121 of p47phox fu…

Available Since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
pVeBtA2d7

Plasmid

#238989

Purpose

Mammalian expression of mutant bovine arrestin-2 long isoform with Venus N-terminal fusion

Depositor

Vsevolod Gurevich

Article

Hanson et al J Mol Biol. 2007 Apr 27;368(2):375-87. d

Insert

arrestin-2 long (ARRB2 Bovine)

Tags

Venus

Expression

Mammalian

Mutation

deleted residues 174, 176, 177, 181-184

Promoter

CMV

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCHC024 (∆SH)

Plasmid

#226202

Purpose

Electroporation knockout plasmid (pK18sB) to delete the C. necator soluble hydrogenase operon (deltaSH).

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for soluble hydrogenase operon

Use

Synthetic Biology

Mutation

∆hoxFUYHWI ∆hypA2B2F2

Available Since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCHC023 (∆MBH)

Plasmid

#226201

Purpose

Electroporation knockout plasmid (pK18sB) to delete the C. necator membrane-bound hydrogenase operon (deltaMBH).

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for membrane bound hydrogenase operon

Use

Synthetic Biology

Mutation

∆hoxKGZMLOQRTV ∆hypA1B1F1CDEX ∆hoxABCJ

Available Since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCHC004 (∆phaCAB)

Plasmid

#226198

Purpose

Electroporation knockout plasmid (pK18sB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for phaCAB

Use

Synthetic Biology

Available Since

Nov. 15, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHR-SNAP-CD86iso2

Plasmid

#223574

Purpose

Lentiviral expression of human CD86 isoform 2 with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

CD86 (CD86 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-SNAP-PD-1 I126A

Plasmid

#223573

Purpose

Lentiviral expression of human PD-1 (I126A) with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

PD-1 (PDCD1 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Mutation

I126A

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-cT3G-cERH-SOHLH1

Plasmid

#222922

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of SOHLH1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

SOHLH1 (SOHLH1 Human)

Expression

Mammalian

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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