We narrowed to 18,275 results for: MUT

1. pAc5.1B-lambdaN-HA-DmNot1_1147-1717-3xL-Amut_P

   Plasmid

   #147171

   Purpose

   Insect Expression of DmNot1_1147-1717-3xL-Amut

   Depositor

   Elisa Izaurralde

   Insert

   DmNot1_1147-1717-3xL-Amut (Not1 Fly)
   

   Expression

   Insect

   Mutation

   2 silent mutations compared to the sequence given…

   Available Since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTRIP TRE BI_SEPT9_i1-14-GFP10_GFP11-14-SEPT7 Gmut2

   Plasmid

   #180359

   Purpose

   mammalian co-expression of human SEPT9_i1 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Inserts

   SEPTIN7 (SEPTIN7 Human)
   SEPTIN 9 (SEPTIN9 Human)
   IRES-TagBFP
   

   Use

   Lentiviral

   Tags

   GFP10 and GFP11

   Expression

   Mammalian

   Mutation

   SEPT7 H279D

   Promoter

   bidirectional TRE

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTRIP TRE BI_SEPT9_i3-14-GFP10_GFP11-14-SEPT7 Gmut2

   Plasmid

   #180361

   Purpose

   mammalian co-expression of human SEPT9_i3 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Inserts

   SEPTIN7 (SEPTIN7 Human)
   SEPTIN9 (SEPTIN9 Human)
   IRES-TagBFP
   

   Use

   Lentiviral

   Tags

   GFP10 and GFP11

   Expression

   Mammalian

   Mutation

   SEPT7 H279D

   Promoter

   bidirectional TRE

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pnEA-vH_His-TEV-SEPT2 NCmut-msfGFP

   Plasmid

   #180311

   Purpose

   bacterial expression of human SEPT2 NCmut fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Insert

   SEPTIN2 (SEPTIN2 Human)
   

   Tags

   His6-TEV and msfGFP

   Expression

   Bacterial

   Mutation

   SEPT2 F20D, V27D

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pnEA-vH_His-TEV-SEPT2 NCmut-msfGFP_SEPT6

   Plasmid

   #180312

   Purpose

   bacterial co-expression of human SEPT2 NCmut fused to monomeric superfolder GFP and of human SEPT6

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Inserts

   SEPTIN2 (SEPTIN2 Human)
   SEPTIN6 (SEPTIN6 Human)
   

   Tags

   His6-TEV and msfGFP

   Expression

   Bacterial

   Mutation

   SEPT2 F20D, V27D

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. K-to-R mutant NPRL3(1-569) in pLJM60, codon-optimized

   Plasmid

   #184563

   Purpose

   CMV-driven expression of an untagged mutant NPRL3 (core subunit of GATOR1) — codon-optimized sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.

   Depositor

   Kacper Rogala

   Article

   Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:

   Insert

   C16orf35, CGTHBA, FFEVF3, HS-40, MARE, NPR3, RMD11 (NPRL3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   All lysines were mutated to arginines. Codon-opti…

   Promoter

   CMV

   Available Since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. psiCHECK2-mMOV10-3'UTR-MRE16+MRE153-mut

   Plasmid

   #178910

   Purpose

   dual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicase

   Depositor

   Constance Ciaudo

   Article

   Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

   Insert

   3'UTR mMov10 (Mov10 Mouse)
   

   Use

   Luciferase

   Mutation

   CTG>AGT and TGC>CAT mir16+153 mutated

   Promoter

   SV40 promoter

   Available Since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut2x

   Plasmid

   #182254

   Purpose

   Human PDAP1 3` UTR region mutated in 2x binding site for miR-150 cloned downstream of luciferase reporter gene

   Depositor

   Silvia Monticelli

   Article

   Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

   Insert

   3' UTR region of PDAP1 gene mutated in 2 binding sites for mir-150
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut1x

   Plasmid

   #182253

   Purpose

   Human PDAP1 3` UTR region mutated in 1x binding site for miR-150 cloned downstream of luciferase reporter gene

   Depositor

   Silvia Monticelli

   Article

   Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

   Insert

   3' UTR region of PDAP1 gene mutated in 1 binding site for mir-150
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut3x

    Plasmid

    #182255

    Purpose

    Human PDAP1 3` UTR region mutated in 3x binding site for miR-150 cloned downstream of luciferase reporter gene

    Depositor

    Silvia Monticelli

    Article

    Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

    Insert

    3' UTR region of PDAP1 gene mutated in 3 binding sites for mir-150
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-Hygro-Ef1A-Myc-POLB(PAMmut)

    Plasmid

    #176150

    Purpose

    N-terminus Myc-tagged POLB containing a mutation in the PAM site used by POLB gRNA1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Mutation in the PAM site used by POLB gRNA1

    Promoter

    EF1A

    Available Since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmirGlo-RBM8A-3'UTRAluY-Sz mut 2598-4066

    Plasmid

    #171135

    Purpose

    reporter

    Depositor

    Emanuela Felley-Bosco

    Article

    Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

    Insert

    RBM8A 3'UTR (RBM8A Human)
    

    Expression

    Mammalian

    Mutation

    A3302G, A3356G, A3373G, A3389G,A3451G, T3729C, A3…

    Available Since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMXS-IRES-Blast 3XFLAG-CHP1-myr_mut

    Plasmid

    #173167

    Purpose

    Retroviral vector to express sgRNA resistant 3XFLAG tagged human CHP1 with myristoylation site mutation

    Depositor

    Kivanc Birsoy

    Article

    Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

    Insert

    Calcineurin Like EF-Hand Protein 1 (CHP1 Human)
    

    Use

    Retroviral

    Tags

    3XFLAG

    Mutation

    Synonymous mutations at sgRNA sites, G2A, S6A

    Promoter

    MMLV

    Available Since

    Aug. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. p5E-161K enhancer-ETS-CORE-MUT

    Plasmid

    #138372

    Purpose

    Gateway compatible 5' entry clone contains human modified 161k enhancer sequence. with ETS binding sites mutated

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    human 161K human enhancer with ETS bind sites mutated
    

    Use

    Gateway compatible

    Mutation

    ETS factor binding sites mutated

    Promoter

    NONE

    Available Since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. dre-cdk2 3'UTR sicheck2 199 mut

    Plasmid

    #121122

    Purpose

    luciferase assay

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    dre-cdk2 3'UTR mutated 199 binding (cdk2 Zebrafish)
    

    Expression

    Bacterial

    Mutation

    miR-199 seed binding site mutated

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Fibronectin-human-mutated N-terminal (N) O-Glycosylation site

    Plasmid

    #120404

    Purpose

    enables eukaryotic expression of human plasma fibronectin in which the first O-glycosylation site was mutated from threonine to serine

    Depositor

    Inaam Nakchbandi

    Article

    Sens et al J Bone Miner Res. 2017 Jan;32(1):70-81.

    Insert

    Fibronectin (FN1 Human)
    

    Expression

    Mammalian

    Mutation

    Containing complete variable region with a mutati…

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPICZ-pOst1-pro-alphaf(MUT2)-E2-Crimson

    Plasmid

    #117663

    Purpose

    Test intracellular localisation of E2-Crimson and study the secretion efficiency

    Depositor

    Pau Ferrer, Benjamin Glick

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pOst1-pro-af(MUT2)-E2-Crimson
    

    Expression

    Yeast

    Mutation

    This plasmid encodes the fluorescent protein E2-C…

    Promoter

    pAOX1

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLex-FLAG-FASTKD4 mutant delta 226

    Plasmid

    #73621

    Purpose

    Expression vector containing FASTKD4 mutant delta 226

    Depositor

    Vamsi Mootha

    Article

    Wolf et al Cell Rep. 2014 May 8;7(3):918-31. doi: 1

    Insert

    FASTKD4 (TBRG4 Human)
    

    Mutation

    Y616A, L617A, K618A, K620A

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. 1287 p3E-mut let-7 etv2 3’ UTR

    Plasmid

    #49011

    Purpose

    Zebrafish Long length etv2 3' UTR with five let7 binding site mutations in gateway 3' Entry vector

    Depositor

    Nathan Lawson

    Article

    Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

    Insert

    ets variant gene 2 (etsrp Zebrafish)
    

    Use

    Gateway 3' entry

    Mutation

    Mutated the Let7 miRNA binding sites

    Available Since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pmyc-GFP-TNRC6A-del.GW-II-NES-mut

    Plasmid

    #42038

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    deleted aa716-772, F951A, F955A, I958A, F960A

    Promoter

    CMV

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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