We narrowed to 17,355 results for: URE

1. sgRNA BRCA2 R2842C

   Plasmid

   #139324

   Purpose

   Plasmid expressing a sgRNA to introduce BRCA2 R2842C using base editing

   Depositor

   Alberto Ciccia

   Article

   Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

   Insert

   sgRNA to insert BRCA2 R2842C using base editing
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJSC228 - Bacterial expression plasmid for SpCas9 Cluster 2 variant

   Plasmid

   #101233

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 2 variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant G582A/V583A/E584A/D585A/N588A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   G582A, V583A, E584A, D585A and N588A

   Promoter

   T7

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBabe-Myc-TRF1iDDR

   Plasmid

   #44576

   Depositor

   Eros Lazzerini Denchi

   Article

   Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

   Insert

   chimeric TRF (TERF2, TERF1 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   TRF1 containing the iDDR domain of TRF2 (aa407-43…

   Promoter

   Available Since

   May 31, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pBabe-Myc-TRFcH

   Plasmid

   #44574

   Depositor

   Eros Lazzerini Denchi

   Article

   Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

   Insert

   chimeric TRF (TERF2, TERF1 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   TRF2 Hinge domain (aa245-443) replaced by that of…

   Promoter

   Available Since

   May 17, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pJSC272 - Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant

   Plasmid

   #101236

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant K918A/V922A/R925A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   K918A, V922A, R925A and Q926A

   Promoter

   T7

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWZLblasti-HA-MEK5

   Plasmid

   #53167

   Purpose

   Expresses HA tagged human MEK5 from blasticidin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK5 (MAP2K5 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pBabe-Myc-TRFcM

   Plasmid

   #44575

   Depositor

   Eros Lazzerini Denchi

   Article

   Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

   Insert

   chimeric TRF (TERF2, TERF1 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   TRF2 DNA-binding domain (aa 444-500) is replaced …

   Promoter

   Available Since

   May 17, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pJSC236 - Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant

   Plasmid

   #101235

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant F491A/M495A/T496A/N497A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   F491A, M495A, T496A, N497A and Q926A

   Promoter

   T7

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJSC239 - Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant

   Plasmid

   #101234

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant T657A/G658A/W659A/R661A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   T657A, G658A, W659A, R661A and Q926A

   Promoter

   T7

   Available Since

   Nov. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJSC269 - Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant

    Plasmid

    #101220

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant N692A/M694A/Q695A/H698A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    N692A, M694A, Q695A, H698A and Q926A

    Promoter

    T7

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJSC197 - Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant

    Plasmid

    #101232

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant G528A/V583A/E584A/D585A/N588A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    G528A, V583A, E584A, D585A, N588A and Q926A

    Promoter

    T7

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKMW121_WT-Cas9_U6-entry

    Plasmid

    #244818

    Purpose

    Mammalian expression of WT Cas9 with Golden Gate-compatible sgRNA spacer cassette

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    SpyCas9 (WT)
    

    Use

    CRISPR

    Tags

    3xFLAG-SV40 NLS and Nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG, U6

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKMW493_U6-sgRNA-entry

    Plasmid

    #244823

    Purpose

    Mammalian expression of SpyCas9 single guide RNA with Golden Gate-compatible sgRNA spacer

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    SpyCas9 single guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHS484_10xHis_MBP_TEV_dSpRY

    Plasmid

    #244832

    Purpose

    Bacterial expression of catalytically inactive SpRY Cas9 (dSpRY) for protein purification

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    dSpRY Cas9
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

    Promoter

    T7

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHS24_10xHis_MBP_TEV_SpRY-Cas9

    Plasmid

    #244827

    Purpose

    Bacterial expression of SpRY Cas9 for protein purification

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    SpRY Cas9
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

    Promoter

    T7

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHS72_10xHis_MBP_TEV_SpG-Cas9_2xNLS

    Plasmid

    #244834

    Purpose

    Bacterial expression of SpG Cas9 with 2xSV40 nuclear localization sequence for protein purification

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    SpG Cas9
    

    Use

    CRISPR

    Tags

    10xHis-MBP and 2xSV40 NLS

    Expression

    Bacterial

    Mutation

    D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

    Promoter

    T7

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Interface-1 (a)

    Plasmid

    #204428

    Purpose

    Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

    Depositor

    Keir Neuman

    Article

    Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

    Insert

    hOpa1 isoform 1 (OPA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R627A K663A R683A

    Promoter

    CMV

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Interface-1 (b)

    Plasmid

    #204429

    Purpose

    Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

    Depositor

    Keir Neuman

    Article

    Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

    Insert

    hOpa1 isoform 1 (OPA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    E626A T630A K668A E671A

    Promoter

    CMV

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Interface-2

    Plasmid

    #204433

    Purpose

    Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

    Depositor

    Keir Neuman

    Article

    Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

    Insert

    hOpa1 isoform 1 (OPA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K614A H615A D835A

    Promoter

    CMV

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Palpha6 and Interface-4 (a)

    Plasmid

    #204434

    Purpose

    Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

    Depositor

    Keir Neuman

    Article

    Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

    Insert

    hOpa1 isoform 1 (OPA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    F860A Y861A Y862A Y863A Q864A R865A H866A F867A

    Promoter

    CMV

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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