We narrowed to 17,203 results for: puro

1. pLVX-TetOne-Puro-hAXL

   Plasmid

   #124797

   Purpose

   Tet-inducible AXL expression in mammalian cells

   Depositor

   Kenneth Pienta

   Article

   Axelrod et al Mol Cancer Res. 2019 Feb;17(2):356-369.

   Insert

   AXL (AXL Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GS

   Available since

   June 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. tet-pLKO-sgRNA-puro

   Plasmid

   #104321

   Purpose

   3rd generation lentiviral plasmid for inducible expression of sgRNA; derived from tet-pLKO-puro; puromycin selection. See manual for detailed protocols.

   Depositor

   Nathanael Gray

   Article

   Huang et al eLife. 2017 Sep 19;6. pii: e26693. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1/TO

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. LentiGuide-puro-NLS-mCherry

   Plasmid

   #185474

   Purpose

   Mammalian Expression, Lentiviral vector expressing mCherry for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-CMV-CD19 puro

   Plasmid

   #196634

   Purpose

   Express CD19

   Depositor

   Nicolas Manel

   Article

   Jeremiah et al J Exp Med. 2023 May 1;220(5):e20220666.

   Insert

   CD19 (CD19 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. P3-dCas9-Tet1-GFP-Puro

   Plasmid

   #190728

   Purpose

   Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)

   Depositor

   Maja Jagodic

   Article

   Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

   Insert

   dCas9-tet1
   

   Use

   CRISPR

   Tags

   3XFLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Changed Pro434 codon from CCG to CCC and Gly468 f…

   Promoter

   pCAG

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. p23-puro-EGFP-RPA194

   Plasmid

   #179237

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Wu et al Science. 2021 Jul 30;373(6554):547-555.

   Insert

   RPA194 (POLR1A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tet-pLKO-puro-Scrambled

   Plasmid

   #47541

   Purpose

   Tet inducible scrambled shRNA.

   Depositor

   Charles Rudin

   Article

   Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

   Insert

   Scrambled shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFH2.155_LV-Puro-T2A-mTagBFP2

   Plasmid

   #205553

   Purpose

   Express puromycin resistance marker and mTagBFP2

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   Puro-T2A-mTagBFP2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti_CMV: Flag-Akaluc_SV40:PuroR

   Plasmid

   #183048

   Purpose

   Expresses FLAG-tagged AkaLuc (Far-Red Firefly luciferase) in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

   Insert

   AkaLuc
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. CROPseq-multi-Puro (pRW1441)

    Plasmid

    #216217

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi construct with Puromycin resistance

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAVS1-Puro CAG FUCCI

    Plasmid

    #136934

    Purpose

    donor plasmid for constitutive FUCCI expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

    Insert

    Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)
    

    Use

    Synthetic Biology

    Tags

    Clover and mKO2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-hCDC6p-Venus-puro

    Plasmid

    #212666

    Purpose

    Express Venus under CDC6 promoter

    Depositor

    Tobias Meyer, Yumi Konagaya

    Article

    Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

    Insert

    hCDC6p-Venus
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hCDC6p

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSIH1-puro-STAT3 shRNA

    Plasmid

    #26596

    Depositor

    Frank Sinicrope

    Article

    Huang et al Mol Cancer Ther. 2010 Mar . 9(3):742-50.

    Insert

    STAT3 shRNA (STAT3 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAVS1-Puro Xlone-eGFP

    Plasmid

    #136936

    Purpose

    donor plasmid for inducible transgene expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

    Insert

    all-in-one tet-on system
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GS

    Available since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. qTAG-AAVS1-PGK-Puro

    Plasmid

    #227269

    Purpose

    Empty AAVS1 targeting donor for the insertion of Puro and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. qTAG-N-Puro-mNeon-ACTB

    Plasmid

    #207752

    Purpose

    Donor template for Puro-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    ACTB Homology Arms flanking a Puro-mNeon Cassette (ACTB Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. qTAG-N-Puro-moxGFP-LMNB1

    Plasmid

    #207773

    Purpose

    Donor template for Puro-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LMNB1 Homology Arms flanking a Puro-moxGFP Cassette (LMNB1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. qTAG-C-moxGFP-Puro-H2BC11

    Plasmid

    #207758

    Purpose

    Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pTet-O-LHX8-T2A-PuroR

    Plasmid

    #162350

    Purpose

    Lentiviral expression of LHX8 under the control of the TetON promoter

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    LHX8 (LHX8 Human)
    

    Use

    Lentiviral

    Tags

    T2A-PuroR

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-ON

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. qTAG-N-Puro-moxGFP-TUBA1B

    Plasmid

    #207767

    Purpose

    Donor template for Puro-2A-moxGFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBA1B Homology Arms flanking a Puro-moxGFP Cassette (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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