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pGL4_EGR1p-BC0132-luc2

Plasmid

#227136

Purpose

Barcoded assay, MAPK sensor; barcode BC0132

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

EGR1 promoter driving barcode 0132 and a luciferase reporter gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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psicheck2-PHLPP2

Plasmid

#203605

Purpose

Reporter plasmid carrying PHLPP2 3'UTR

Depositor

Hiroyuki Mizuguchi

Article

Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

Insert

PH domain and leucine rich repeat protein phosphatase 2 (PHLPP2 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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psicheck2-27bT

Plasmid

#203600

Purpose

Reporter plasmid carrying miR-27b target sequence

Depositor

Hiroyuki Mizuguchi

Article

Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

Insert

complementary sequence to miR27b-3p (MIR27B Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pQC.Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)]-Fluc

Plasmid

#200124

Purpose

Retroviral expression of Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)] protease reporter and P2A firefly luciferase in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)] P2A firefly luciferase

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pQC.Flip-nluc(LgBiT1-8)CP[CoVA]-Fluc

Plasmid

#200125

Purpose

Retroviral expression of Flip-nluc(LgBiT1-8)CP[CoVA] protease reporter and P2A firefly luciferase in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

Flip-nluc(LgBiT1-8)CP[CoVA] P2A firefly luciferase

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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psiCHECK-NP

Plasmid

#196607

Purpose

Dual-Luciferase Reporter vector carrying a 425bp-long Influenza virus NP sequence in forward direction

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Insert

nucleocapsid protein (NP) gene, partial

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-mMOV10-3'UTR-MRE16+MRE153-mut

Plasmid

#178910

Purpose

dual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicase

Depositor

Constance Ciaudo

Article

Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

Insert

3'UTR mMov10 (Mov10 Mouse)

Use

Luciferase

Tags

Expression

Mutation

CTG>AGT and TGC>CAT mir16+153 mutated

Promoter

SV40 promoter

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pPLN-hFluc-WT-KDEL

Plasmid

#177715

Purpose

Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

hFluc

Use

Tags

KDEL and Prolactin Signal Sequence

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pSI-check2-Cul1b-3'UTR

Plasmid

#97153

Purpose

For dual luciferase report assay

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

Cul1b 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSI-check2-Traf6-3'UTR

Plasmid

#97154

Purpose

For dual luciferase report assay

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

Traf6 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-CB-Nluc-P2A-copGFP-T2A-Puro

Plasmid

#73039

Purpose

Express Nluc, copGFP dual reporter

Depositor

Kazuhiro Oka

Insert

Nluc-P2A-copGFP-T2A-Puro

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

chicken beta-actin + CMV enhancer and chicken bet…

Available since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pMV306hsp+LuxG13

Plasmid

#26161

Depositor

Brian Robertson, Siouxsie Wiles

Article

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

Bacterial luciferase operon + G13 promoter

Use

Tags

Expression

Bacterial

Mutation

It contains a gram-positive enhanced translation …

Promoter

Available since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only

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C035

Plasmid

#174341

Purpose

Template for making Rluc mRNA reporter with pA120.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

Renilla luciferase

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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C098

Plasmid

#174345

Purpose

Template for making Nluc mRNA reporter with pA139.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

NanoLuc luciferase

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
C034

Plasmid

#174340

Purpose

Template for making Rluc mRNA reporter with pA98.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

Renilla luciferase

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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C033

Plasmid

#174339

Purpose

Template for making Rluc mRNA reporter with pA63.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

Renilla luciferase

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
C031

Plasmid

#174338

Purpose

Template for making Rluc mRNA reporter with pA30.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

Renilla luciferase

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
C054

Plasmid

#174342

Purpose

Template for making Fluc mRNA reporter with pA124 and mutant mouse Malat1.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

Firefly luciferase

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

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C071

Plasmid

#174343

Purpose

Template for making Nluc mRNA reporter with pA29.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

NanoLuc luciferase

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
C093

Plasmid

#174344

Purpose

Template for making Nluc mRNA reporter with pA63.

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

NanoLuc luciferase

Use

In vitro transcription template

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge