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pSLQ14806

Plasmid

#239273

Purpose

Expresses TRF1-PYL1 for recruiting RNA to the nuclear stress body via CRISPR-TO

Depositor

Stanley Qi

Article

Han et al Nature. 2025 Jul;643(8070):241-251. doi:

Insert

HSF1-BFP-PYL1 (HSF1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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SP6-VEE-GFP

Plasmid

#58976

Purpose

expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Insert

EGFP

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

26S subgenomic promoter

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hADARB2-3xFLAG

Plasmid

#165470

Purpose

Human ADARB2 mammalian expression vector with 3 C-terminal FLAG tags.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

Adenosine deaminase RNA specific B2 (inactive) (ADARB2 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hHP1BP3-HA

Plasmid

#165468

Purpose

Human HP1BP3 mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

Heterochromatin protein 1 binding protein 3 (HP1BP3 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro_shDDX58_230212

Plasmid

#167293

Purpose

Lentiviral plKO.1 construct coding for a short-hairpin RNA targeting the human gene DDX58 (coding for RLR sensor RIG-I). Contains a puro resistance cassette.

Depositor

Agnel Sfeir

Article

Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

Insert

shRNA hsDDX58 (RIGI Human)

Use

Lentiviral

Expression

Mammalian

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pet28a-His6-Halo-TEV-Nrf2

Plasmid

#62455

Purpose

Halo-His6 tagged Nrf2 for E.coli expression

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Nrf2 (NFE2L2 Human)

Tags

His6-Halo-TEV

Expression

Bacterial

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S

Plasmid

#62458

Purpose

expresses his-tagged C151S human Keap1 protein

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Tags

His-Halo-Tev

Expression

Mammalian

Mutation

Changed Cysteine 151 of Keap1 to serine

Promoter

CMV

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAcCas12nHS_RV6_PT1_empty

Plasmid

#203810

Purpose

Plasmid containing a CMV-driven NLS-AcCas12n-PT1-NLS(human-codonoptimized)cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion). Carbenicillin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcCas12nHS_RV6_PT1

Use

CRISPR

Promoter

CMV

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX307 ASC

Plasmid

#163977

Purpose

Mammalian expression vector encoding ASC (for example, to assess ASC-speck formation or inflammasome signalling assays)

Depositor

Daniel Bachovchin

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

ASC (PYCARD Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-mScarlet-Control-5'UTR

Plasmid

#184637

Purpose

Fluorescent reporter for mammalian cells: Expression of mScarlet with a CMV 5'UTR

Depositor

Hao Wu

Article

Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

mScarlet

Use

Lentiviral

Expression

Mammalian

Available Since

June 2, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-Syn-SomArchon

Plasmid

#126941

Purpose

Expression of SomArchon under Syn promoter

Depositor

Edward Boyden

Article

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Synapsin

Available Since

June 24, 2019

Availability

Academic Institutions and Nonprofits only

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Stat1 beta Flag pRc/CMV

Plasmid

#8704

Depositor

Jim Darnell

Article

Schindler et al Proc Natl Acad Sci U S A 1992 Aug 15;89(

Insert

STAT1 beta (STAT1 Human)

Tags

Flag Tag

Expression

Mammalian

Available Since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only
Stat1 alpha pRC/CMV

Plasmid

#8690

Depositor

Jim Darnell

Article

Schindler et al Proc Natl Acad Sci U S A 1992 Aug 15;89(

Insert

STAT1 alpha (STAT1 Human)

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pSH231-Bx-GFP-C31

Plasmid

#115149

Purpose

Empty safe harbor site 231 knock-in vector with flanking homology arms and Bxb1 and C31 attP integrase landing pads

Depositor

Raymond Monnat, Michael Phelps

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

Safe Harbor Homology Sequences

Expression

Mammalian

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pB-CAG-FLEX-F14F15-BFP-(mCherry)'

Plasmid

#201831

Purpose

piggyBac transposon plasmid for making Cre and Flpo reporter cell lines using piggyBac transposase

Depositor

Ian Wickersham

Article

Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Inserts

CAG
BFP

Expression

Mammalian

Promoter

CAG

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-SomArchon

Plasmid

#126942

Purpose

Expression of SomArchon under CaMKII promoter

Depositor

Edward Boyden

Article

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

CaMKII

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-TWIN-STREP-FLAG-NPRL3

Plasmid

#164984

Purpose

NPRL3 expressing vector for mammalian cells

Depositor

Roberto Zoncu

Article

Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

Insert

NPRL3 (NPRL3 Human)

Tags

2xSTREP FLAG

Expression

Mammalian

Promoter

CAG

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hDDX50-HA

Plasmid

#165465

Purpose

Human DDX50 mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

DExD-box helicase 50 (DDX50 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pSH231-EFS-Cas9-BlastR

Plasmid

#115146

Purpose

Safe harbor site 231 knock-in vector with Cas9-BlastR expression cassette

Depositor

Raymond Monnat, Michael Phelps

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

Cas9-T2A-BlastR

Use

CRISPR

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-GST FNIP2

Plasmid

#164982

Purpose

FNIP2 expressing vector for mammalian cells

Depositor

Roberto Zoncu

Article

Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

Insert

FNIP2 (FNIP2 Human)

Tags

GST

Expression

Mammalian

Promoter

CAG

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pMIR-Dsred-IRES-His-Halo-Tev-Keap C288S

Plasmid

#62459

Purpose

expresses his-tagged C288S human Keap1 protein

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Tags

His-Halo-Tev

Expression

Mammalian

Mutation

Changed Cysteine 288 of Keap1 to serine

Promoter

CMV

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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Stat1 beta pRc/CMV

Plasmid

#8703

Depositor

Jim Darnell

Article

Schindler et al Proc Natl Acad Sci U S A 1992 Aug 15;89(

Insert

STAT1 beta (STAT1 Human)

Expression

Mammalian

Available Since

July 20, 2005

Availability

Academic Institutions and Nonprofits only
pCA15-Ubc-NLS- scFV-tdTomato

Plasmid

#199446

Purpose

Expression of scFV-tdTomato that binds to the GCN4 peptide from the SunTag System in mammalian cells

Depositor

Baohui Chen

Article

Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

Insert

scFV-tdTomato

Use

Lentiviral

Expression

Mammalian

Promoter

human ubiquitin C (UBC)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG GST DEPDC5

Plasmid

#164983

Purpose

DEPDC5 expressing vector for mammalian cells

Depositor

Roberto Zoncu

Article

Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

Insert

DEPDC5 (DEPDC5 Human)

Tags

GST

Expression

Mammalian

Promoter

CAG

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-mScarlet-MAVS-5'UTR

Plasmid

#184639

Purpose

Fluorescent reporter for mammalian cells: Expression of mScarlet with MAVS 5'UTR

Depositor

Hao Wu

Article

Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

mScarlet

Use

Lentiviral

Expression

Mammalian

Available Since

June 2, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pSH231-EF1-BLST-Cas9-VPR

Plasmid

#115147

Purpose

Safe harbor site 231 knock-in vector with BlastR-Cas9-VPR expression cassette

Depositor

Raymond Monnat, Michael Phelps

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

BlastR-P2A-Cas9-VPR

Use

CRISPR

Tags

SV40NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A RIGI K270A

Plasmid

#167290

Purpose

Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving catalytically dead (K270A) RLR sensor RIG-I

Depositor

Agnel Sfeir

Article

Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

Insert

DDX58 (RIGI Human)

Use

Lentiviral

Tags

tagBFP

Expression

Mammalian

Mutation

K270A

Promoter

SFFV

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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Zebrafish-gRNA-0005

Plasmid

#42245

Purpose

gRNA targeted to zebrafish gene gsk3b

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

gRNA-gsk3b (gsk3b Zebrafish)

Use

CRISPR; Zebrafish expression

Promoter

T7

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-mCherry-T2A-miniSOG-C-Far

Plasmid

#193915

Purpose

Expresses miniSOG targeted to the plasma membrane of neurons, joined by a T2A cleavage site with mCherry; for AAV production

Depositor

Xiao Wang

Article

Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

Insert

miniSOG

Use

AAV

Tags

Farnesylation signal sequence and Flag tag

Expression

Mammalian

Promoter

hSyn

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET11-PC-H6

Plasmid

#183777

Purpose

Express PC-H6 in E. coli. Purified PC-H6 was used for for generating antibodies in rabbits and for GST-CBP pull-down assays.

Depositor

Peter Harte

Article

Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

Insert

Polycomb (Pc Fly)

Tags

6 His tag at the C-terminus

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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