We narrowed to 4,048 results for: GAST
-
Plasmid#148333PurposeBacterial Expression of DmNanos-opt_116-163-L127DF130DDepositorInsertDmNanos-opt_116-163-L127DF130D (nos Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-AvrII-dsRNAres_L
Plasmid#146826PurposeInsect Expression of DmGW182-dsRNAresDepositorInsertDmGW182-dsRNAres (gw Fly)
UseTagsExpressionInsectMutationTwo non silent mutations V704A and N799S and one …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-dsRNAres_L
Plasmid#146831PurposeInsect Expression of DmGW182-dsRNAresDepositorInsertDmGW182-dsRNAres (gw Fly)
UseTagsExpressionInsectMutationTwo non silent mutations V704A and N799S and one …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-3-GST-DmGW182_1-593_F
Plasmid#146271PurposeBacterial Expression of DmGW182_1-593DepositorInsertDmGW182_1-593 (gw Fly)
UseTagsExpressionBacterialMutationone non silent mutation T1772A (L591Q) compared t…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmeIF4G_578-634-GB1_AG
Plasmid#148744PurposeBacterial Expression of DmeIF4G_578-634DepositorInsertDmeIF4G_578-634 (eIF4G1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-41-DmPABPC1_J
Plasmid#146643PurposeBacterial Expression of DmPABPC1DepositorInsertDmPABPC1 (pAbp Fly)
UseTagsExpressionBacterialMutation4 silent mutations compared to the sequence geven…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpH-DmCaf40_25-291-Y139DG146W_AF
Plasmid#148736PurposeBacterial Expression of DmCaf40_25-291-Y139DG146WDepositorInsertDmCaf40_25-291-Y139DG146W (Rcd-1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-MBP-GGSG-DmBam_13-36_AG
Plasmid#148841PurposeMammalian Expression of DmBam_13-36DepositorInsertDmBam_13-36 (bam Fly)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-DmRoquin-del725-755-del790-812_AE
Plasmid#148634PurposeMammalian Expression of DmRoquin-del725-755-del790-812DepositorInsertDmRoquin-del725-755-del790-812 (roq Fly)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
dora[B]_rescue_construct
Plasmid#190637PurposePuromycin-selectable expression of Dora[T295M] (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTagsExpressionInsectMutationChanged threonine 295 to methioninePromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-dora[B]_rescue_construct
Plasmid#190638PurposePuromycin-selectable expression of HA-tagged Dora[T295M] (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTags3xHAExpressionInsectMutationChanged threonine 295 to methioninePromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
dora[A]_rescue_construct
Plasmid#190639PurposePuromycin-selectable expression of truncated Dora (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTagsExpressionInsectMutationTruncated to contain amino acids 1-945PromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-dora[A]_rescue_construct
Plasmid#190640PurposePuromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTags3xHAExpressionInsectMutationTruncated to contain amino acids 1-945PromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBABR mCherry-Bcd-LEXY
Plasmid#182596PurposeDrosophila integration plasmid expressing mCherry-Bcd-LEXY fusion protein under the maternal tubulin promoter.DepositorInsertBcd (bcd Fly)
UseTagsLEXY and mCherryExpressionInsectMutationPromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBABR Bcd-LEXY
Plasmid#182594PurposeDrosophila integration plasmid expressing Bcd-LEXY fusion protein under the maternal tubulin promoter.DepositorInsertBicoid (bcd Fly)
UseTagsLEXYExpressionInsectMutationPromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmDCP1-N41AS100A-dsRNAres_P
Plasmid#147215PurposeInsect Expression of DmDCP1-N41AS100A-dsRNAresDepositorInsertDmDCP1-N41AS100A-dsRNAres (Dcp1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmDCP1-Y34F-dsRNAres_P
Plasmid#147217PurposeInsect Expression of DmDCP1-Y34F-dsRNAresDepositorInsertDmDCP1-Y34F-dsRNAres (Dcp1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmXRN1_1312-1355-DmDCP1_1-127_N
Plasmid#147054PurposeBacterial Expression of DmXRN1_1312-1355-DmDCP1_1-127DepositorInsertDmXRN1_1312-1355-DmDCP1_1-127 (pcm Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmXRN1_1323-1355-DmDCP1_1-127_N
Plasmid#147055PurposeBacterial Expression of DmXRN1_1323-1355-DmDCP1_1-127DepositorInsertDmXRN1_1323-1355-DmDCP1_1-127 (pcm Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmXRN1_1323-1383-DmDCP1_1-127_N
Plasmid#147056PurposeBacterial Expression of DmXRN1_1323-1383-DmDCP1_1-127DepositorInsertDmXRN1_1323-1383-DmDCP1_1-127 (pcm Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
puc19_N_tag
Plasmid#186650PurposeEmpty donor plasmid.DepositorInsertN-terminal 3xFlag-3xHA Tag
UseTags3xFlag-3xHAExpressionInsectMutationPromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTL464
Plasmid#69881Purposea dCirl transcriptional reporter allele that contains an optimized gal4.2::p65 cassette at the start codon of the genomic dCirl ORFDepositorInsertCIRL (Cirl Fly)
UsePhic31-integration vectorTagsExpressionMutationPromoterAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCaf40-A253FY208A_X
Plasmid#147973PurposeInsect Expression of DmCaf40-A253FY208ADepositorInsertDmCaf40-A253FY208A (Rcd-1 Fly)
UseTagsEGFP (A179V)ExpressionInsectMutationPromoterAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4E-T_1-511-LLFWKtoAAAAA-V5His6_Z
Plasmid#148160PurposeInsect Expression of Dm4E-T_1-511-LLFWKtoAAAAADepositorInsertDm4E-T_1-511-LLFWKtoAAAAA (4E-T Fly)
UseTagsExpressionInsectMutationalias: 4E-T; one non silent mutation L127S, annot…PromoterAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-CBP(1-287)
Plasmid#182839PurposeCBP(1-287) was inserted into pGEX-2T with NdeI and NsiI sites. pGEX-2T-CBP(1-287) was used in the GST pull-down assay described in our paper (Tie et al. 2012 MCB, Tie et al 2014 Development)DepositorInsertCBP1-287
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET11-PC(75-390)-H6
Plasmid#183781PurposeExpress PC(75-390)-H6 in E. coli. PC(75-390)-H6 was used for GST-CBP pull-down assaysDepositorInsertPolycomb (w Fly)
UseTags6 His tag at the C-terminusExpressionBacterialMutation6 His tag at the C-terminusPromoterT7Available sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHelz-E958Q_AH
Plasmid#148884PurposeInsect Expression of DmHelz-E958QDepositorInsertDmHelz-E958Q (CG9425 Fly)
UseTagsExpressionInsectMutationtwo silent an two non silent G1374S, R2076G mutat…PromoterAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
1098E=TI-pgSIT[tra,bTub,Hasp70Bb-Cas9]
Plasmid#149427PurposeThe attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.DepositorInsertsU6.3-gRNAs[TraB, bTub]
Hsp70Bb-Cas9-T2A-eGFP-p10
UseCRISPRTagseGFPExpressionInsectMutationPromoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_50-83-GB1_Z
Plasmid#148180PurposeBacterial Expression of DmThor_50-83DepositorInsertDmThor_50-83 (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHelz_1213-2103_AH
Plasmid#148883PurposeInsect Expression of DmHelz_1213-2103DepositorInsertDmHelz_1213-2103 (CG9425 Fly)
UseTagsExpressionInsectMutationtwo silent an two non silent G1374S, R2076G mutat…PromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN2-F1103A-dsRNAres_Y
Plasmid#148075PurposeInsect Expression of DmPAN2-F1103A-dsRNAresDepositorInsertDmPAN2-F1103A-dsRNAres (PAN2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCaf40-F65AC69Y_X
Plasmid#147993PurposeInsect Expression of DmCaf40-F65AC69YDepositorInsertDmCaf40-F65AC69Y (Rcd-1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-GST-DmNanos_116-163-E151AF152AN155A-V5His6_AC
Plasmid#148466PurposeInsect Expression of DmNanos_116-163-E151AF152AN1555ADepositorInsertDmNanos_116-163-E151AF152AN1555A (nos Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCaf40_25-291_W
Plasmid#147866PurposeInsect Expression of DmCaf40_25-291DepositorInsertDmCaf40_25-291 (Rcd-1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot2-del458-482-dsRNAres_U
Plasmid#147716PurposeInsect Expression of DmNot2-del458-482-dsRNAresDepositorInsertDmNot2-del458-482-dsRNAres (Rga Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot2-del393-429-W556E-dsRNAres_U
Plasmid#147698PurposeInsect Expression of DmNot2-del393-429-W556E-dsRNAresDepositorInsertDmNot2-del393-429-W556E-dsRNAres (Rga Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmeIF4G_601-660-GB1_AD
Plasmid#148506PurposeBacterial Expression of DmeIF4G_601-660DepositorInsertDmeIF4G_601-660 (eIF4G1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpG-DmNanos-opt_116-163-F152E_AB
Plasmid#148332PurposeBacterial Expression of DmNanos-opt_116-163-F152EDepositorInsertDmNanos-opt_116-163-F152E (nos Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmPAN2-K1124EY1127EK1131E-dsRNAres_Y
Plasmid#148046PurposeBacterial Expression of DmPAN2-K1124EY1127EK1131E-dsRNAresDepositorInsertDmPAN2-K1124EY1127EK1131E-dsRNAres (PAN2 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-MGW-Cterm_H
Plasmid#146484PurposeInsect Expression of DmGW182-MGW-CtermDepositorInsertDmGW182-MGW-Cterm (gw Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmDCP1-Y34A-dsRNAres_P
Plasmid#147216PurposeInsect Expression of DmDCP1-Y34A-dsRNAresDepositorInsertDmDCP1-Y34A-dsRNAres (Dcp1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-F594V-siRNAres_F
Plasmid#146331PurposeInsect Expression of DmAGO1-F594VDepositorInsertDmAGO1-F594V (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations compared to…PromoterAvailable sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmEDC3_1-101-V5His6_D
Plasmid#146121PurposeInsect Expression of DmEDC3_1-101DepositorInsertDmEDC3_1-101 (Edc3 Fly)
UseTagsExpressionInsectMutationone silent mutation A456G and three mutations N16…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmEDC3_1-338-V5His6_D
Plasmid#146122PurposeInsect Expression of DmEDC3_1-338DepositorInsertDmEDC3_1-338 (Edc3 Fly)
UseTagsExpressionInsectMutationone silent mutation A456G and three mutations N16…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmEDC3_441-680_D
Plasmid#146127PurposeInsect Expression of DmEDC3_441-680DepositorInsertDmEDC3_441-680 (Edc3 Fly)
UseTagsExpressionInsectMutation3 mutations N162T, K221E and D418G compared to th…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmEDC3_441-680_D
Plasmid#146130PurposeInsect Expression of DmEDC3_441-680DepositorInsertDmEDC3_441-680 (Edc3 Fly)
UseTagsExpressionInsectMutationone silent mutation A456G and three mutations N16…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmEDC3-D61A_D
Plasmid#146131PurposeInsect Expression of DmEDC3-D61ADepositorInsertDmEDC3-D61A (Edc3 Fly)
UseTagsExpressionInsectMutationone silent mutation A456G and three mutations N16…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmEDC3-D61K_D
Plasmid#146132PurposeInsect Expression of DmEDC3-D61KDepositorInsertDmEDC3-D61K (Edc3 Fly)
UseTagsExpressionInsectMutationone silent mutation A456G and three mutations N16…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmEDC3-N44A_D
Plasmid#146135PurposeInsect Expression of DmEDC3-N44ADepositorInsertDmEDC3-N44A (Edc3 Fly)
UseTagsExpressionInsectMutationone silent mutation A456G and three mutations N16…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-RB_B
Plasmid#145977PurposeInsect Expression of DmAGO1-RBDepositorInsertDmAGO1-RB (AGO1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only