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CMV-Hylight

Plasmid

#193447

Purpose

A fluorescent biosensor for fructose 1,6-bisphosphate (FBP). Changes in FBP concentration strongly correlate with changes in glycolytic flux. (mammalian expression vector)

Depositor

Richard Goodman

Article

Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

Insert

Hylight Biosensor

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-CMV-Luc2-IRES-ZsGreen-W

Plasmid

#164432

Purpose

Lentiviral vector backbone encoding firefly luciferase and ZsGreen

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Inserts

Luciferase
ZsGreen

Use

Lentiviral

Available Since

Feb. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro (PX459) V2.0

Plasmid

#62988

Purpose

Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9-2A-Puro V2.0

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

Cbh

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV2/2

Plasmid

#232199

Purpose

AAV packaging plasmid expressing AAV2 Rep and capsid proteins

Depositor

Melina Fan, Addgene Research Program

Insert

Rep2/Cap2

Use

AAV

Expression

Mammalian

Available Since

Sept. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pKC117_pLenti_TetOff_MVP_IRES_PABPC1_INT

Plasmid

#242113

Purpose

Tet- or Dox-repressible expression of MVP and PABPC1 fused to INT (human PARP4 fragment)

Depositor

Fei Chen

Article

Chao et al Science. 2026 Jan 15:eadz9353. doi: 10.1

Insert

MVP and PAB4L-PARP4 fragment (MVP, PABPC1 Synthetic)

Use

Lentiviral

Mutation

PABPC1 aa 1-370, PARP4 aa 1562-1724

Promoter

TRE3G

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti CMV GFP Puro (658-5)

Plasmid

#17448

Purpose

3rd gen lentiviral eGFP expression vector, CMV promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pST39-pNL29

Plasmid

#91696

Purpose

Expresses Mega GVs in E.Coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Protoc. 2017 Oct;12(10):2050-2080. d

Insert

Mega GV gene cluster (from pNL29) in pST39 backbone

Tags

No tags

Expression

Bacterial

Promoter

T7

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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attB-puro donor.dna

Plasmid

#181923

Purpose

attB-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attB-EGFP-EF1α-PuroR-T2A-TagBFP

Expression

Mammalian

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-P2A-GFP

Plasmid

#222996

Purpose

Mammalian expression of SpCas9 PE7 prime editor with P2A-EGFP marker

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7-P2A-EGFP

Tags

P2A-EGFP, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-dCas9-KRAB-blast

Plasmid

#89567

Purpose

Plasmid expression dCas9 protein in fusion with KRAB domain

Depositor

Gary Hon

Article

Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

Insert

dCas9

Use

Lentiviral

Tags

Blasticidin resistance gene and KRAB

Mutation

D10A and H840A

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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