Addgene

We narrowed to 20,869 results for: Tes

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pLVX-Flag-Polß(V303R)-IRES-puro

Plasmid

#128659

Purpose

mammalian expression of Flag-Polß(V303R) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

V303R

Promoter

CMV

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(K72A)-IRES-puro

Plasmid

#128661

Purpose

mammalian expression of Flag-Polß(K72A) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

K72A

Promoter

CMV

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCT-CMV-copGFP-Polß(WT)-puro

Plasmid

#128665

Purpose

mammalian expression of copGFP-Polß(WT) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

copGFP

Expression

Mammalian

Mutation

Wild type

Promoter

CMV

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(K244A)-IRES-puro

Plasmid

#128682

Purpose

mammalian expression of Flag-Polß(K244A) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

K244A

Promoter

CMV

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(L301R)-IRES-puro

Plasmid

#128654

Purpose

mammalian expression of Flag-Polß(L301R) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

L301R

Promoter

CMV

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(K206A)-IRES-puro

Plasmid

#128678

Purpose

mammalian expression of Flag-Polß(K206A) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

K206A

Promoter

CMV

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(V306R)-IRES-puro

Plasmid

#128655

Purpose

mammalian expression of Flag-Polß(V306R) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

V306R

Promoter

CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDRf1-4CL5-AtSDT (JBEI-11535)

Plasmid

#87934

Purpose

Co-expression in S.cerevisiae of 4CL5 and AtSDT

Depositor

Dominique Loque

Article

Eudes et al Microb Cell Fact. 2016 Nov 21;15(1):198.

Inserts

SDT (SDT Mustard Weed)
4CL5 (4CL5 Mustard Weed)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pM-ErbB4-delta-kinase-PY1 mutant

Plasmid

#17801

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

ErbB4 (ERBB4 Human)

Use

Tags

GAL4-BD

Expression

Mammalian

Mutation

Carboxy-terminal fragment, amino acids 988-1292, …

Promoter

Available since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
GAL-hAR-658-919

Plasmid

#89082

Purpose

mammalian expression of human androgen receptor ligand binding domain 658-919 amino acids linked to GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2007 Aug 31;282(35):25801-1

Insert

hAR-658-919 (AR Human)

Use

Tags

GAL4 DBD

Expression

Mammalian

Mutation

aa 658-919

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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HIP-flash (HOP-flash mutant)

Plasmid

#83466

Purpose

Negative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter gene

Depositor

Barry Gumbiner

Article

Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

Insert

Multimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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Lentiviral mBmal1-Luc

Plasmid

#182762

Purpose

Lentiviral vector encoding the promoter region of mouse Bmal1 gene driving a luciferase reporter

Depositor

Qing-Jun Meng

Article

Gossan et al Arthritis Rheum. 2013 Sep;65(9):2334-45.

Insert

mouse arntl promoter (Bmal1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

649 bp

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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tetO-Hand2

Plasmid

#46028

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

Hand2 (Hand2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

July 22, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5 Myc-tagged PSK

Plasmid

#197149

Purpose

Expression of MYC-tagged PSK1-alpha / TAOK2 in mammalian cells

Depositor

Jonathan Morris

Article

Moore et al J Biol Chem. 2000 Feb 11;275(6):4311-22.

Insert

TAOK2 (TAOK2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Wild Type

Promoter

SV40

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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hFLT1

Plasmid

#83435

Purpose

human VEGFR1 with HA and FLAG tag

Depositor

Christie Thomas

Article

Raikwar et al Exp Cell Res. 2013 Oct 15;319(17):2578-8

Insert

human VEGFR1 (FLT1 Human)

Use

Tags

Flag and Myc

Expression

Mammalian

Mutation

HA-tag was inserted 30 AA downstream of the FLT1 …

Promoter

CMV

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST RBM14-V5

Plasmid

#69823

Purpose

Expresses RBM14-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

RNA-binding protein 14 (RBM14 Human)

Use

Retroviral

Tags

v5

Expression

Mammalian

Mutation

Promoter

cmv

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pDIV313

Plasmid

#204956

Purpose

AMA1 plasmid with Aspergillus optimized Mad7, hph (hygromycin) resistance marker and sgRNA targeting albA locus in A. niger

Depositor

Uffe Mortensen

Article

Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

Inserts

Mad7
hph (hygromycin resistance marker)
sgRNA (CAGCAATGCTTCCATGCAATT) targeting albA locus in A. niger flanked by a tRNA-Gly repeat

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Mutation

Promoter

Aspergillus fumigatus U3 promoter, Aspergillus ni…

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pET-15b-hAR-663-919

Plasmid

#89083

Purpose

bacterial expression of human androgen receptor ligand bindng domain amino acid residues 663-919 with histidine tag

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2007 Aug 31;282(35):25801-1

Insert

hAR ligand binding domain (AR Human)

Use

Tags

His

Expression

Bacterial

Mutation

aa 663-919

Promoter

T7

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-eGFP-NLS-RNF168

Plasmid

#133977

Purpose

mammalian expression vector of eGFP tagged wild type RNF168

Depositor

Daniel Durocher

Article

Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

Insert

RNF168 (RNF168 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

siRNA resistant sequence: GAGGAGTCGTGTTTATTGA

Promoter

CMV

Available since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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FUW TetO HA-AMPKa1 WT

Plasmid

#69824

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

minimal CMV promoter with tetracycline operator

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-Sec22b-shR

Plasmid

#208358

Purpose

Mammalian expression of fluorescent N-terminally tagged shRNA resistant Sec22b

Depositor

Paula Nunes-Hasler

Article

Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

Insert

Sec22b (Sec22b Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

four silent mutations introduced to render shRNA …

Promoter

CMV

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST MTFR1L-V5

Plasmid

#69822

Purpose

Expresses MTFR1L-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Mitochondrial fission regulator 1-like (MTFR1L Human)

Use

Retroviral

Tags

v5

Expression

Mammalian

Mutation

Promoter

cmv

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pLVH-EF1a-GFP-P2A-OCT4

Plasmid

#130692

Purpose

Lentiviral plasmids encoding human OCT4 (POU5f1) along with GFP linked via P2A.

Depositor

Kejin Hu

Article

Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

Insert

human OCT4 (POU5F1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2ΔKinase gRNA resistant

Plasmid

#163411

Purpose

Expresses BMPR-2ΔKinase resistant to the gRNAs(#163388-90) in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

The kinase domain (202-500 aa) is deleted. gRNA-t…

Promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLVH-EF1a-GFP-P2A-KLF4

Plasmid

#130694

Purpose

Lentiviral plasmids encoding human KLF4 with co-expressoin of GFP mediated by P2A.

Depositor

Kejin Hu

Article

Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

Insert

human KLF4 (KLF4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCAP03-sp44/p21

Plasmid

#120232

Purpose

Carries bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites that can be amplified with homology sequence for refactoring.

Depositor

Bradley Moore

Article

Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

Insert

bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites

Use

Synthetic Biology

Tags

Expression

Mutation

aac(3)IV (apramycin resistance gene)

Promoter

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pLPC-FLAG.Mm.DMAP1

Plasmid

#184650

Purpose

Retroviral expression of mouse DMAP1

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

Dmap1 (Dmap1 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pcFLAG-CSK-delta SH3 (73 to450 amino acids)

Plasmid

#74504

Purpose

Overexpression of CSK delta SH3 in mammalian cells

Depositor

Lars Rönnstrand

Article

Kazi et al Cell Signal. 2013 Sep;25(9):1852-60. doi

Insert

CSK (CSK Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

del-SH3 (73 to450 amino acids)

Promoter

CMV

Available since

May 2, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA4-His-Max-A-PQBP1

Plasmid

#25802

Depositor

Marius Sudol

Article

Tapia et al J Biol Chem. 2010 Apr 21. ():.

Insert

PQBP1 (PQBP1 Human)

Use

Tags

His and Xpress

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2010

Availability

Academic Institutions and Nonprofits only
FUW TetO HA-AMPKa1 AS

Plasmid

#69825

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

minimal CMV promoter with tetracycline operator

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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