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KHBD00699
Plasmid#39736DepositorInsertCG12690 (CHES-1-like Fly)
UseGateway donr vectorTagsExpressionMutationPromoternoneAvailable sinceAug. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pDONR223_IKBKE_WT
Plasmid#82248PurposeGateway Donor vector containing IKBKE, part of the Target Accelerator Plasmid Collection.DepositorInsertIKBKE (IKBKE Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3/V5-DEST-GFP
Plasmid#40125DepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA-human MYCN
Plasmid#74163PurposeMammalian expression of human MycNDepositorInsertMycN (MYCN Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR R4-vas2-integrase-R3
Plasmid#62299PurposephageC31 integrase-expressing helper plasmid for Anopheles transgenesis, vasa promoterDepositorInsertphageC31 integrase under control of vasa promoter and terminator
UseTagsHA and SV40 NLSExpressionInsectMutationPromotervasaAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ING2
Plasmid#13294DepositorInsertING2 (INGX Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pENTR L1-vas2-Cre-L4
Plasmid#62301PurposeCre recombinase-expressing helper plasmid for Anopheles gene engineering, vasa promoterDepositorInsertCre recombinase under control of vasa promoter and terminator
UseTagsHA and SV40 NLSExpressionInsectMutationPromotervasaAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1A-Dm-mir-6-1,2,3
Plasmid#22789DepositorInsertmicro RNA 6-1, micro RNA 6-2, micro RNA 6-3 (mir-6-1 primary transcript, Fly)
UseDrosophila expressionTagsExpressionMutationPromoterAvailable sinceDec. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
AIRAP 5: AIRAP(1-50).GST,V1.pcDNA3
Plasmid#21795DepositorInsertAIRAP (Zfand2a Mouse)
UseTagsGSTExpressionMammalianMutationamino acids 1-50PromoterAvailable sinceSept. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
FtsZ-Dendra2
Plasmid#92038PurposeExpress FtsZ-Dendra2DepositorInsertFtsZ
UseTagsDendra2ExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
HDAC5 Flag
Plasmid#13822PurposeMammalian expression of human histone deacetylase 5 with flag tagDepositorInsertHDAC5 (HDAC5 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF1alpha-pcDNA3
Plasmid#18949DepositorInsertHypoxia inducible factor 1 alpha (HIF1A Human)
UseTagsHA-tagExpressionMammalianMutationwt cDNAPromoterAvailable sinceSept. 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRGEN-CMV-CjCas9
Plasmid#89752PurposeExpression of CjCas9 in mammalian cellsDepositorInsertCas9
UseCRISPRTagsSV40-HAExpressionMammalianMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-BG505.SOSIP-gp160
Plasmid#123273PurposeMammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations)DepositorInsertHIV-1 (BG505) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; SOSIP mutationsPromoterCMVAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
ER-ABKAR
Plasmid#61508PurposeExpression of AMPK biosensor targeted to the endoplasmic reticulumDepositorInsertAMPK Biosensor
UseTagsER targeting sequence from CYP450 2C1 (see commen…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
PM-ABKAR
Plasmid#61506PurposeExpression of AMPK biosensor targeted to the plasma membrane via Lyn kinase targeting sequenceDepositorInsertAMPK Biosensor
UseTagsLyn kinase sequence (see comments)ExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/Capsid
Plasmid#120816PurposeEctopic expression of ZIKV proteinDepositorInsertCapsid ZIKV
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-BaLgp140-TM
Plasmid#111843PurposeMammalian expression plasmid for extracellular gp140 from the BaL HIV-1 strain fused to a transmembrane tetherDepositorInsertHIV-1 (BaL) Env
UseTagsCD5 leader sequence and TM helix from MHC class IExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pECIA2
Plasmid#47032PurposeThis is a Gateway destination vector for secreted expression in Drosophila cell culture by induction via CuSO4. Inserts will be C-terminally tagged with Fc, V5 and hexahistidine tags.DepositorTypeEmpty backboneUseSecreted expression in drosophila cultureTagsFc(human IgG1), HRV 3C Protease Cleavage site, He…ExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceAug. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/Envelope
Plasmid#120821PurposeEctopic expression of ZIKV proteinDepositorInsertEnvelope ZIKV
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/pr-Membrane
Plasmid#120817PurposeEctopic expression of ZIKV proteinDepositorInsertpr-Membrane ZIKV
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/EV
Plasmid#120833PurposeControl vector, 20nt random sequence between gateway recombination sitesDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/NS5
Plasmid#120825PurposeEctopic expression of ZIKV proteinDepositorInsertNS5 ZIKV
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/NS2A
Plasmid#120818PurposeEctopic expression of ZIKV proteinDepositorInsertNS2A ZIKV
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/NS4A
Plasmid#120826PurposeEctopic expression of ZIKV proteinDepositorInsertNS4A ZIKV
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/NS4B
Plasmid#120823PurposeEctopic expression of ZIKV proteinDepositorInsertNS4B ZIKV
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/NS1
Plasmid#120824PurposeEctopic expression of ZIKV proteinDepositorInsertNS1 ZIKV
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/NS5-YFP
Plasmid#120831PurposeEctopic expression of ZIKV proteinDepositorInsertZIKV NS5-eYFP
UseTagseYFP-V5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only
