We narrowed to 11,909 results for: pcDNA

1. pcDNA5-FRT-TO-Nsp10-HF

   Plasmid

   #157708

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp10-HF (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pcDNA5FRT/TO-UBXD7-Strep-HA

   Plasmid

   #113479

   Purpose

   Expression of human UBXD7 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   UBXD7 (UBXN7 Human)
   

   Use

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   A834C silent mutation, wildtype without stop codon

   Promoter

   CMV

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-NPY-ClopHensor

   Plasmid

   #25939

   Depositor

   Daniele Arosio

   Article

   Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

   Insert

   NPY-ClopHensor (NPY Aq. Victoria and Discosoma sp)
   

   Use

   Tags

   NPY and Strep-II

   Expression

   Mammalian

   Mutation

   Neuropeptide Y localization signal

   Promoter

   Available since

   Sept. 13, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA5FRT/TO-UBE2QL1-CS-Strep/HA

   Plasmid

   #129513

   Purpose

   Expresses UBE2QL1 C88S (catalytically inactive mutant) with C-terminal Strep-HA tag in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

   Insert

   UBE2QL1 C88S (UBE2QL1 Human)
   

   Use

   Tags

   2xStrep/HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNAintron-LASV-GPC

   Plasmid

   #215690

   Purpose

   Produces the Lassa virus glycoprotein

   Depositor

   Melinda Brindley

   Article

   Acciani et al J Virol. 2017 Aug 24;91(18):e00574-17. d

   Insert

   LASV GP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pcDNA3.1(+)mGAT1-0-GFP

   Plasmid

   #41662

   Depositor

   Henry Lester

   Article

   Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

   Insert

   Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   C-terminal hydrophobic isoleucine residue added a…

   Promoter

   CMV

   Available since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 1533 pcDNA3 HA MARS

   Plasmid

   #10716

   Depositor

   William Sellers

   Insert

   MARS (MARS1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcdna3 Flag JIP3b

   Plasmid

   #53458

   Purpose

   interacts with MAPK signaling module

   Depositor

   Roger Davis

   Article

   Kelkar et al Mol Cell Biol. 2000 Feb;20(3):1030-43.

   Insert

   mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA-Golgi-ZapCY1

   Plasmid

   #36322

   Depositor

   Amy Palmer

   Article

   Qin et al Proc Natl Acad Sci U S A. 2011 May 3;108

   Insert

   Golgi-ZapCY1 high affinity Zn(II) sensor
   

   Use

   Tags

   GalT (first 60 amino acids)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 31, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-Myc S6K1

    Plasmid

    #26610

    Depositor

    Jie Chen

    Article

    Fang et al Science. 2001 Nov 30. 294(5548):1942-5.

    Insert

    S6K1 (Rps6kb1 Rat)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1-CHRFAM7A-mCherry

    Plasmid

    #62635

    Purpose

    mammalian expression of human duplicated Alpha7 (CHRFAM7A) with mCherry fused into M3-M4 loop

    Depositor

    Henry Lester

    Article

    Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

    Insert

    CHRFAM7A (CHRFAM7A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mCherry inserted into M3-M4 loop

    Promoter

    CMV

    Available since

    March 20, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA5FRT/TO-FAF1-Strep-HA

    Plasmid

    #113486

    Purpose

    Expression of human FAF1 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    FAF1 (FAF1 Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1(+)-mCherry-p115

    Plasmid

    #181996

    Purpose

    Mammalian expression vector encoding p115 RhoGEF tagged with mCherry.

    Depositor

    Jin Zhang

    Article

    Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

    Insert

    mCherry-p115 (ARHGEF1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-Nrxn1beta AS4(-)-Fc

    Plasmid

    #59313

    Purpose

    Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]

    Depositor

    Tito Serafini, Peter Scheiffele

    Article

    Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.

    Insert

    Nrxn1beta AS4(-)-Fc fusion (Nrxn1 Mouse)
    

    Use

    Tags

    Fc (from human IgG)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-Dronpa-Kras

    Plasmid

    #182329

    Purpose

    Plasma membrane-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    Dronpa-Kras
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-AKAP79-Dronpa

    Plasmid

    #182332

    Purpose

    AKAP79-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    AKAP79-Dronpa (AKAP5 Human)
    

    Use

    Tags

    Full-length AKAP79

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA3 Flag MKK7B2Jnk3a2

    Plasmid

    #19729

    Depositor

    Roger Davis

    Article

    Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

    Insert

    MKK7B2Jnk3a2 (Map2k7, MAPK10 Human, Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA5/FRT/TO_LAMP1_HaloTag7

    Plasmid

    #175531

    Purpose

    Lyzosomal expression of HaloTag7 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    LAMP1-HaloTag7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GNAS_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176976

    Purpose

    Mammalian expression vector encoding GNAS and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    GNAS (GNAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MRAP_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176937

    Purpose

    Mammalian expression vector encoding MRAP and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    MRAP (MRAP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3-His-SUMO1

    Plasmid

    #159323

    Purpose

    Mammalian cell expression of mouse His-SUMO1

    Depositor

    Jianjun Zhao

    Insert

    His-SUMO1 (Sumo1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA FLAG TORC3

    Plasmid

    #22976

    Depositor

    Marc Montminy

    Article

    Screaton et al Cell. 2004 Oct 1. 119(1):61-74.

    Insert

    TORC3 (CRTC3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

23. pCDNA5 TEX264wt-cSSH

    Plasmid

    #220209

    Purpose

    Mammalian expression of wild type TEX264, C-terminal twin-Strep_HA tag

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Use

    Tags

    Strep-strep-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1-MS2-HA-HshnRNPK_Z

    Plasmid

    #148124

    Purpose

    Mammalian Expression of HshnRNPK

    Depositor

    Elisa Izaurralde

    Insert

    HshnRNPK (HNRNPK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3 MDM2 S166D

    Plasmid

    #16234

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ser166 replaced by Asp

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

26. pcDNA-DDX11-Flag-WT

    Plasmid

    #120727

    Purpose

    Expression of a Flag-tagged version of DDX11 WT in mammalian cells

    Depositor

    Francesca Pisani

    Article

    Cortone et al PLoS Genet. 2018 Oct 10;14(10):e1007622.

    Insert

    DDX11 (DDX11 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCDNA3-myc3-FBW5

    Plasmid

    #19905

    Depositor

    Yue Xiong

    Article

    Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

    Insert

    F-box and WD repeat domain containing 5 (FBXW5 Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3-erExRai-AKAR2

    Plasmid

    #161756

    Purpose

    ExRai-AKAR2 targeted to the cytosolic face of the ER membrane.

    Depositor

    Jin Zhang

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    CYP450-ExRai-AKAR2
    

    Use

    Tags

    N-terminal 27 amino acids from CYP450

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3-sACE2v2.4(732)-8h

    Plasmid

    #154103

    Purpose

    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (8his tag)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Short GSG linker and 8his tag

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-G…

    Promoter

    CMV

    Available since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA5-FRT-TO-Nsp9

    Plasmid

    #157703

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp9 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp9 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pcDNA5/FRT/TO V5 DNAJB6a

    Plasmid

    #19529

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    DNAJB6a (DNAJB6 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. h-Plekhm1_FLAG-pcDNA 3.1

    Plasmid

    #73593

    Purpose

    Express human Plekhm1 in mammalian cells

    Depositor

    Paul Odgren

    Article

    Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3.1_GEM22-Halo-FRB

    Plasmid

    #197059

    Purpose

    Expression of Mycobacterium hassiacum encapsulin Halo-FRB-fusion in mammalian cells

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    Encapsulin
    

    Use

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3-AktAR-Kras

    Plasmid

    #123357

    Purpose

    FRET-based biosensor for monitoring Akt activity in non-raft plasma membrane regions.

    Depositor

    Jin Zhang

    Article

    Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

    Insert

    AktAR-Kras
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3-sACE2v1-Fc(IgG1)

    Plasmid

    #145164

    Purpose

    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity variant 1

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Fc region of human IgG1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3.T7 delta-NFAT3

    Plasmid

    #28224

    Depositor

    Sebastien Jauliac

    Article

    Fougere et al Oncogene. 2010 Apr 15. 29(15):2292-301.

    Insert

    Delta NFAT3 (NFATC4 Human)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    hNFAT3 WT deleted amino acids 1-342

    Promoter

    CMV

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3-β2AR-μDpTT

    Plasmid

    #182326

    Purpose

    Beta-2 adrenergic receptor-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    beta2AR-μDronpa-TagRFP-T (ADRB2 Human)
    

    Use

    Tags

    Full-length human beta2 adrenergic receptor

    Expression

    Mammalian

    Mutation

    Dronpa contains H193T mutation.

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3-RIIα-μDpTT

    Plasmid

    #182327

    Purpose

    PKA RIIα subunit-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    RIIalpha-μDronpa-TagRFP-T (PRKAR2A Human)
    

    Use

    Tags

    Full-length human PKA type 2alpha regulatory subu…

    Expression

    Mammalian

    Mutation

    Dronpa contains H193T mutation.

    Promoter

    CMV

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3-H2B-μDpTT

    Plasmid

    #182328

    Purpose

    Histone H2B-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    H2B-μDronpa-TagRFP-T (H2BC3 Human)
    

    Use

    Tags

    Full-length human histone H2B

    Expression

    Mammalian

    Mutation

    Dronpa contains H193T mutation.

    Promoter

    CMV

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3-Lifeact-Dronpa

    Plasmid

    #182330

    Purpose

    Actin-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    Lifeact-Dronpa
    

    Use

    Tags

    N-terminal 17 amino acids from budding yeast Abp1…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge