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pcDNA5FRT/TO-Npl4-Strep-HA

Plasmid

#113495

Purpose

Expression of human Npl4 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

Npl4 (NPLOC4 Human)

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-RAPGEF2

Plasmid

#110162

Purpose

Expression of human RAPGEF2 in mammalian cells

Depositor

Daniele Guardavaccaro

Article

Magliozzi et al Dev Cell. 2013 Dec 9;27(5):574-85. doi:

Insert

RAPGEF2 (Rap guanine nucleotide exchange factor 2 (RAPGEF2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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iRFP-Sec61beta

Plasmid

#108125

Purpose

ER marker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

iRFP:SEC61B (SEC61B Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3 1 kb prom. + Enhancer CD274

Plasmid

#107005

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Expression

Mammalian

Mutation

N.B. There are two published SNPs in this region …

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Tubulin K40Q

Plasmid

#105302

Purpose

To study effects of acetyl-mimetic (hyper-acetylated) tubulin

Depositor

Kenneth Yamada

Article

Joo et al Nat Commun. 2014 Mar 25;5:3510. doi: 10.

Insert

alpha tubulin (TUBA1B Human)

Tags

EGFP

Expression

Mammalian

Mutation

K40Q

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hygro(+)-PSTCD-cGAS

Plasmid

#102605

Purpose

Mammalian expression of cGAS fused to PSTCD tag

Depositor

Nicolas Manel

Article

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Insert

cGAS (CGAS Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-GST-4EBP1-Low GC

Plasmid

#69018

Purpose

expresses tagged 4EBP1

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

EIF4EBP1 (EIF4EBP1 Human)

Tags

GST and HA

Expression

Mammalian

Mutation

lowGC content, wt is ~70% GC rich. Still, it code…

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pETDuet RagA-RagC

Plasmid

#99641

Purpose

Bacterial expression of wildtype Rag GTPase dimer

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCLXSN-EphA2-Flag

Plasmid

#102755

Purpose

Expression of EphA2

Depositor

Jin Chen

Article

Song et al Cancer Res. 2014 May 1;74(9):2444-54. do

Insert

EphA2 (EPHA2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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AICSDP-25:MAP1LC3B-mEGFP

Plasmid

#101783

Purpose

Homology arms and mEGFP-linker sequence for N-terminus tagging of human MAP1LC3B

Depositor

Allen Institute for Cell Science

Insert

MAP1LC3B Homology Arms with mEGFP-linker (MAP1LC3B Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

Oct. 31, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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