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We narrowed to 8,506 results for: Ott

Showing: 1891 - 1920 of 8506 results

  6. AP1muA-TurboID-polyA-G418 HR

    Plasmid
    #227734
    Purpose
    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells.
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al J Cell Sci. 2024 Aug 15;137(16):jcs26195
    Insert
    genomic sequence AP1M1 locus (AP1M1 Human)
    Use
    CRISPR
    Tags
    Turbo-ID, V5
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    227734-plasmid-map-sequence-id-457074

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  8. pFP5299 ss-FIREmate-HA- KDEL-IVS-IRES- ManII-SBP- mApple-FIREtag

    Plasmid
    #216695
    Purpose
    Synchronize anterograde and retrograde trafficking of ManII between ER and Golgi (RUCH system)
    Depositor
    Franck Perez
    Article
    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s
    Inserts
    FIREmate-KDEL
    ManII-SBP-mApple-FIREtag (Man2a1 Mouse)
    Use
    Tags
    FIREtag, Streptavidin Binding Protein (SBP), and …
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 116 of ManII
    Promoter
    CMV
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    216695-plasmid-map-sequence-id-429706

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  12. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA

    Plasmid
    #134925
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.
    Depositor
    Tomoji Mashimo
    Article
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Insert
    Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134925-plasmid-map-sequence-id-267605

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  13. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA

    Plasmid
    #134922
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.
    Depositor
    Tomoji Mashimo
    Article
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Insert
    Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134922-plasmid-map-sequence-id-267588

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  14. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA

    Plasmid
    #134924
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.
    Depositor
    Tomoji Mashimo
    Article
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Insert
    Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134924-plasmid-map-sequence-id-267600

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  15. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA

    Plasmid
    #134923
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.
    Depositor
    Tomoji Mashimo
    Article
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Insert
    Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134923-plasmid-map-sequence-id-267593

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  17. pCJ202

    Plasmid
    #162675
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating W159C and S238C mutations.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    W159C, S238C; codon optimized for expression in E…
    Promoter
    T7/lac
    Available since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162675-plasmid-map-sequence-id-321470

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Showing: 1891 - 1920 of 8506 results