We narrowed to 78,677 results for: TRI

1. pcDNA3-HA-ERK2 L198A L232A

   Plasmid

   #8978

   Depositor

   John Blenis

   Article

   Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

   Insert

   ERK2 (Mapk1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   L198A, L232A

   Promoter

   Available since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

2. pECFP-Rac1Y40C

   Plasmid

   #105293

   Purpose

   Control for FRET biosensor analysis - cannot bind downstream effectors

   Depositor

   Kenneth Yamada

   Article

   Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

   Insert

   Rac1 (RAC1 Human)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Y40C

   Promoter

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Delta DED FLIP-L

   Plasmid

   #13790

   Depositor

   Guy Salvesen

   Article

   Boatright et al Biochem J. 2004 Sep 1. 382(Pt 2):651-7.

   Insert

   DELTA DED FLIP LONG (CFLAR Human)
   

   Use

   Tags

   8 x His tag

   Expression

   Bacterial

   Mutation

   Delta DED removes first 186 residues

   Promoter

   Available since

   Jan. 25, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQnl_tet

   Plasmid

   #191355

   Purpose

   Clostridium expression vector with TetR-repressed NanoLuc expression

   Depositor

   Andrew Tolonen

   Article

   Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

   Insert

   PGusA2-TetO2/1-Nanoluc, miniPthl-tetR cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PGus2A-TetO2/1

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGWB21_TaNAM-A1

   Plasmid

   #124572

   Purpose

   Expresses full-length TaNAM-A1 with an N-terminal 10x Myc tag under the 35s promoter.

   Depositor

   Cristobal Uauy

   Article

   Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

   Insert

   TaNAM-A1
   

   Use

   Tags

   10xMyc

   Expression

   Plant

   Mutation

   Promoter

   35s

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHMWGFPR

   Plasmid

   #64396

   Purpose

   High molecular weight glutenin gene promoter driving GFP expression

   Depositor

   Claudia Vickers

   Article

   Vickers et al Plant Mol Biol. 2006 Sep;62(1-2):195-214

   Insert

   High molecular weight glutenin gene promoter
   

   Use

   Tags

   GFP

   Expression

   Plant

   Mutation

   Promoter

   High molecular weight glutenin gene promoter

   Available since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pECFP-Cdc42-Y40C

   Plasmid

   #105295

   Purpose

   Control for FRET biosensor analysis - cannot bind downstream effectors

   Depositor

   Kenneth Yamada

   Article

   Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

   Insert

   Cdc42 (CDC42 Human)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Y40C

   Promoter

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSB1A3 EcoRI Methylase-AmilCP

   Plasmid

   #85166

   Purpose

   For methylating EcoRI recognition sites in order to prevent cleavage by the EcoRI restriction enzyme. AmilCP is a blue reporter for Methylase expression.

   Depositor

   Robin Dowell

   Article

   Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71

   Insert

   EcoRI Methylase-AmilCP
   

   Use

   Tags

   AmilCP

   Expression

   Bacterial

   Mutation

   Promoter

   J23100 promoter

   Available since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pAAV-DR1-NRSE-RFP

   Plasmid

   #22927

   Depositor

   Edward Callaway

   Article

   Nathanson et al Front Neural Circuits. 2009 . 3():19.

   Insert

   peroxisome proliferator activated receptor gamma binding site promoter with neuron-restrictive silencer element with minimal CMV
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMT3-RhoGC

    Plasmid

    #100031

    Purpose

    This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein.

    Depositor

    Daniel Oprian

    Article

    Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

    Insert

    RhoGC
    

    Use

    Tags

    1D4 T9'A (AETSQVAPA) and C8 (PRGPDRPEGIEE)

    Expression

    Mammalian

    Mutation

    Promoter

    Adenovirus major late promoter

    Available since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.15 Sense

    Plasmid

    #46839

    Purpose

    Express cGFP transcript ending in a mutant MALAT1 triple helix that causes the transcript to be stable but poorly translated

    Depositor

    Phil Sharp

    Article

    Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

    Insert

    Coral Green Fluorescent Protein (cGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.14 Sense

    Plasmid

    #46838

    Purpose

    Express cGFP transcript ending in the minimal functional MALAT1 triple helix

    Depositor

    Phil Sharp

    Article

    Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

    Insert

    Coral Green Fluorescent Protein (cGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pOCC230

    Plasmid

    #118871

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease and mCitrine, cl…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRII-TOPO CMV-cGFP-mMEN b_3' Sense

    Plasmid

    #46840

    Purpose

    Express cGFP transcript ending in the wildtype MEN beta triple helix

    Depositor

    Phil Sharp

    Article

    Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

    Insert

    Coral Green Fluorescent Protein (cGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHRIG-S6K

    Plasmid

    #59446

    Purpose

    lentiviral expression of S6K and EGFP

    Depositor

    Heng Zhao

    Article

    Xie et al Stroke. 2014 Jul 10. pii: STROKEAHA.114.

    Insert

    S6K (Rps6kb1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    S6K starts at aa24 and contains 7 extra amino aci…

    Promoter

    CMV

    Available since

    Oct. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pCRII-TOPO CMV-cGFP-mMALAT1_3' Mut U1/U2 Sense

    Plasmid

    #46837

    Purpose

    Express cGFP transcript ending in a mutant MALAT1 triple helix which causes the transcript to be degraded

    Depositor

    Phil Sharp

    Article

    Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

    Insert

    Coral Green Fluorescent Protein (cGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. hnRNPAB X2-RBD

    Plasmid

    #216516

    Purpose

    Expresses MBP-tagged RNA binding domain of hnRNPAB X2

    Depositor

    Nicolas Fawzi

    Article

    O'Connell et al Sci Rep. 2024 Nov 13;14(1):27890. doi: 1

    Insert

    hnRNPAB X2-RBD
    

    Use

    Tags

    6xHis tags-MBP

    Expression

    Bacterial

    Mutation

    deleted amino acids 2-64 and 226-304

    Promoter

    Available since

    Dec. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDGO186N-KS1

    Plasmid

    #174301

    Purpose

    Replicating plasmid with sgRNA cassette containing a killing spacer #1 targeting the wild type polC gene.

    Depositor

    Lee Lynd

    Article

    Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

    Insert

    spacer expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDGO186N-KS2

    Plasmid

    #174302

    Purpose

    Replicating plasmid with sgRNA cassette containing a killing spacer #2 targeting the wild type polC gene.

    Depositor

    Lee Lynd

    Article

    Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

    Insert

    spacer expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQmod3S-GG

    Plasmid

    #191349

    Purpose

    Clostridium expression vector (pCB102 origin, specR) with drop-out Plac-RFP cassette flanked with BsaI sites

    Depositor

    Andrew Tolonen

    Article

    Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

    Insert

    Plac-RFP (IPTG-inducible red fluorescent protein)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDGO186N-KS3

    Plasmid

    #174303

    Purpose

    Replicating plasmid with sgRNA cassette containing a killing spacer #3 targeting the wild type polC gene.

    Depositor

    Lee Lynd

    Article

    Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

    Insert

    spacer expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCR8_TaNAM-A1

    Plasmid

    #124574

    Purpose

    TaNAM-A1 CDS in the Gateway-compatible pCR8 vector.

    Depositor

    Cristobal Uauy

    Article

    Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

    Insert

    TaNAM-A1
    

    Use

    Topo gateway cloning vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. UBQ10:sXVE:ATG-3xHA-S11

    Plasmid

    #108238

    Purpose

    Binary vectors used for the inducible expression of tripartite split-sfGFP components (3xHA-S11 as control) in plants

    Depositor

    Tzu-Yin Liu

    Article

    Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

    Insert

    ATG-3xHA-S11
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    UBQ10:sXVE:

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEGFP-N1-FLAG

    Plasmid

    #60360

    Purpose

    Plasmid for cloning and expression of FLAG-GFP tagged proteins (C-terminal tag). Confers resistance to G418.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28 His6-mEGFP-D4H

    Plasmid

    #226373

    Purpose

    Production of recombinant mEGFP-D4H, a fluorescent probe binding cholesterol

    Depositor

    Catherine Tomasetto

    Article

    Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

    Insert

    D4H (pfoA Clostridium perfringens)
    

    Use

    Tags

    His6 and monomeric EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pC1-SypHer3s-IMS

    Plasmid

    #108120

    Purpose

    Mitochondrial ratiometric pH probe with enhanced brightness fused to Smac/DIABLO fragment. Probe is localized in mitochondrial intermembrane space. Mammalian cell expression.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

    Insert

    SypHer3s-IMS
    

    Use

    Tags

    ratiometricYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GPD-roGFP

    Plasmid

    #49436

    Purpose

    Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial intermembrane space of mammalian cells

    Depositor

    Paul Schumacker

    Article

    Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

    Insert

    GPD-roGFP2
    

    Use

    Adenoviral

    Tags

    Glycerol phosphate dehydrogenase, 5' to roGF…

    Expression

    Mammalian

    Mutation

    C48S, Q80R, S147C, and Q204C in Clontech's G…

    Promoter

    CMV

    Available since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pdCas12b(RuvC)

    Plasmid

    #186450

    Purpose

    Encodes catalytically inactive Cas12b (E848A, D977A) from A. acidoterrestris (type V-B)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    dCas12b
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    E848A, D977A

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV-dLight1.1

    Plasmid

    #111053

    Purpose

    Expresses dLight1.1 in mammalian cells

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Insert

    dLight1.1
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDuBir-Lbu-dCas13a-avitag

    Plasmid

    #100817

    Purpose

    Dual expression of Lbu-dCas13a with a C-terminal avi-tag and BirA to biotinylate Lbu-dCas13a

    Depositor

    Mitchell O'Connell

    Article

    Tambe et al Cell Rep. 2018 Jul 24;24(4):1025-1036. d

    Insert

    Lbu_Cas13a_R472A_H477A_R1048A_H1053A
    

    Use

    Tags

    AviTag and His6-MBP

    Expression

    Bacterial

    Mutation

    R472A, H477A, R1048A, H1053A

    Promoter

    T7

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge