We narrowed to 3,258 results for: Ubc

1. AiP1045-pAAV-mscRE10-minBGpromoter-iCre-WPRE-hGHpA

   Plasmid

   #163477

   Purpose

   Direct-expressing iCre AAV Virus. Alias: AiP1045 - pAAV-AiE2010m-minBG-iCre-WPRE-HGHpA

   Depositor

   Bosiljka Tasic, The Allen Institute for Brain Science

   Article

   Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

   Insert

   iCre
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Beta Globin minimal promoter

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. AiP11496 - pAAV-hDlxI56i-minBG-H2B-nsSYFP2-WPRE3-BGHpA (Alias: CN1496)

   Plasmid

   #164449

   Purpose

   Direct-expressing H2BSYFP2 AAV Virus

   Depositor

   Boaz Levi, The Allen Institute for Brain Science

   Article

   Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

   Insert

   H2B-SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBetaGlobin

   Available since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. AiP1046-pAAV-mscRE13-minBGpromoter-iCre-WPRE-hGHpA

   Plasmid

   #163478

   Purpose

   Direct-expressing iCre AAV Virus. Alias: AiP1046 - pAAV-AiE2013m-minBG-iCre-WPRE-HGHpA

   Depositor

   Bosiljka Tasic, The Allen Institute for Brain Science

   Article

   Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

   Insert

   iCre
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Beta Globin minimal promoter

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. YFP(1-158)-beta-2 in pcDNAI/Amp

   Plasmid

   #54469

   Purpose

   An amino-terminal YFP fragment was fused to Gbeta-2. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.

   Depositor

   Catherine Berlot

   Article

   Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

   Insert

   YFP(1-158)/beta-2 (GNB2 Aequorea victoria, Human)
   

   Use

   Tags

   YFP(1-158) was fused to the amino terminus of Gbe…

   Expression

   Mammalian

   Mutation

   YFP (1-158) includes a substitution of Met for Gl…

   Promoter

   CMV

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. YFP(1-158)-beta-5 in pcDNAI/Amp

   Plasmid

   #54470

   Purpose

   An amino-terminal YFP fragment was fused to Gbeta-5. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.

   Depositor

   Catherine Berlot

   Article

   Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

   Insert

   YFP(1-158)/beta-5 (GNB5 Aequorea victoria, Human)
   

   Use

   Tags

   YFP(1-158) was fused to the amino terminus of Gbe…

   Expression

   Mammalian

   Mutation

   YFP (1-158) includes a substitution of Met for Gl…

   Promoter

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. AIMTOR T757

   Plasmid

   #140828

   Purpose

   AIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cells

   Depositor

   Anne Bonnieu

   Article

   Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1

   Insert

   AIMTOR T757 (ULK1 Human)
   

   Use

   Tags

   NES Nuclear export signal

   Expression

   Mammalian

   Mutation

   The insert comprises only a small sequence of hum…

   Promoter

   Available since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSUMO1

   Plasmid

   #52258

   Purpose

   Bacterial expression of human 6His-tagged SUMO1, SUMO-E1 and SUMO-E2

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Inserts

   SUMO-1 (SUMO1 Human)
   UBE2l (UBE2I Human)
   SAE-2 (UBA2 )
   SAE-1 (SAE1 Human)
   

   Use

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   C-terminal gly-gly

   Promoter

   T7 and rbs

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. AiP11851-pAAV-hDLX-minBG-iCre-4X2C-WPRE3-BGHpA (Alias: CN1851)

   Plasmid

   #164450

   Purpose

   AAV vector for Cre expression in forebrain GABAergic interneurons. For use in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors.

   Depositor

   Jonathan Ting, The Allen Institute for Brain Science

   Article

   Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

   Has Service

   AAV PHP.eB

   Insert

   iCre
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBetaGlobin

   Available since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244)

   Plasmid

   #202553

   Purpose

   Nontargeting control vector with TagBFP2

   Depositor

   Frederic Meunier

   Article

   Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

   Insert

   Humanized dCas9-KRAB-TagBFP2 (tag blue fluorescent protein 2) (TRIM28 S. Pyogenes (dCas9), H. sapiens (KRAB), Entacmaea quadricolor (TagBFP2))
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

   Expression

   Mutation

   Puromycin-resistance gene in the pLV hU6-sgRNA hU…

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. P3-Lenti-dCas9-Tet1-GFP

    Plasmid

    #190729

    Purpose

    Lentiviral construct to express spdCas9-Tet1-GFP.

    Depositor

    Maja Jagodic

    Article

    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

    Insert

    dCas9-tet1
    

    Use

    CRISPR

    Tags

    SV40 NLS and T2A-EGFP

    Expression

    Mammalian

    Mutation

    Changed Pro434 codon from CCG to CCC and Gly468 f…

    Promoter

    UbC

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pInducer10b-HA-KRAS WT CO SR

    Plasmid

    #164926

    Purpose

    Expresses HA tagged, codon Optimized and siRNA-resistant, tetracycline-inducible KRAS wild type sequence

    Depositor

    Ji Luo

    Article

    Yuan et al Cancer Discov. 2014 Oct;4(10):1182-1197.

    Insert

    KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)
    

    Use

    Lentiviral

    Tags

    HA tag

    Expression

    Mutation

    9 Valine codons are optimized and siRNA targetin…

    Promoter

    Ubc promoter

    Available since

    May 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pInducer10b-HA-KRAS G12V CO SR

    Plasmid

    #164928

    Purpose

    Expresses HA tagged, codon Optimized and siRNA-resistant, tetracycline-inducible KRASG12V sequence

    Depositor

    Ji Luo

    Article

    Yuan et al Cancer Discov. 2014 Oct;4(10):1182-1197.

    Insert

    KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)
    

    Use

    Lentiviral

    Tags

    HA tag

    Expression

    Mutation

    Glycine 12 changed to valine; 9 Valine codons are…

    Promoter

    Ubc promoter

    Available since

    May 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pInducer(p20) x mKate2-NLS-P2A-HRas(N17)_ires-puro

    Plasmid

    #167832

    Purpose

    Inducible expression of dominant negative HRas(N17) and P2A-coupled reporter mKate2-NLS

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    HRAS(N17) (HRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    S17N

    Promoter

    Ubc, TRE

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Luc.Cre empty vector

    Plasmid

    #20905

    Depositor

    Tyler Jacks

    Type

    Empty backbone

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJC224

    Plasmid

    #231169

    Purpose

    Empty Module A plasmid compatible with MoClo and Cermak (2017) genome engineering plasmids

    Depositor

    Daniel Voytas

    Type

    Empty backbone

    Use

    Subcloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJC225

    Plasmid

    #231170

    Purpose

    Empty Module B plasmid compatible with MoClo and Cermak (2017) genome engineering plasmids

    Depositor

    Daniel Voytas

    Type

    Empty backbone

    Use

    Subcloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJC227

    Plasmid

    #231172

    Purpose

    Empty Module C' plasmid compatible with MoClo and Cermak (2017) genome engineering plasmids

    Depositor

    Daniel Voytas

    Type

    Empty backbone

    Use

    Subcloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Lenti-LucS

    Plasmid

    #22778

    Depositor

    Tyler Jacks

    Insert

    SIY
    

    Use

    Cre/Lox and Lentiviral

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTJK368

    Plasmid

    #138006

    Purpose

    BirA* expression. Vector also expresses GFP under control of hUBC promoter

    Depositor

    Tami Kingsbury

    Insert

    BirA*
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lenti-LucOS

    Plasmid

    #22777

    Depositor

    Tyler Jacks

    Insert

    OVA-SIY
    

    Use

    Cre/Lox and Lentiviral

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. 2PWQ

    Plasmid

    #51353

    Depositor

    Cheryl Arrowsmith

    Insert

    2PWQ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Truncation: See Comments

    Promoter

    T7

    Available since

    April 4, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

22. FUCas9GFP

    Plasmid

    #85555

    Purpose

    Expresses Cas9 with green fluorescent reporter

    Depositor

    Marco Herold

    Insert

    humanised S.pyogenes Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    hUBC

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. pTJK422

    Plasmid

    #138002

    Purpose

    SIX2 expression. Vector also expresses GFP under control of hUBC promoter

    Depositor

    Tami Kingsbury

    Insert

    SIX2 (SIX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. FUCas9Cyan

    Plasmid

    #85554

    Purpose

    Expresses Cas9 with fluorescent Cyan reporter

    Depositor

    Marco Herold

    Insert

    humanised S.pyrogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    hUBC

    Available since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. pTJK299

    Plasmid

    #138000

    Purpose

    SIX1 expression. Vector also expresses GFP under control of hUBC promoter

    Depositor

    Tami Kingsbury

    Insert

    SIX1 (SIX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. FUGW mplum

    Plasmid

    #128071

    Purpose

    3rd gen lentiviral plasmid with hUbC-driven mPlum

    Depositor

    Oskar Laur

    Insert

    mplum
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    no

    Promoter

    Available since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FUGW-EYFP

    Plasmid

    #128074

    Purpose

    3rd gen lentiviral plasmid with hUbC-driven EYFP

    Depositor

    Oskar Laur

    Insert

    EYFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    no

    Promoter

    Available since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTJK477

    Plasmid

    #138005

    Purpose

    3xMYC-SIX2 expression. Vector also expresses GFP under control of hUBC promoter

    Depositor

    Tami Kingsbury

    Insert

    3xMYC tagged SIX2 (SIX2 Human)
    

    Use

    Lentiviral

    Tags

    3xMYC

    Expression

    Mutation

    None

    Promoter

    Available since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. FUW-mTie1

    Plasmid

    #119234

    Purpose

    Plasmid encodes full-length murine Tie1 gene cloned in lentiviral vector.

    Depositor

    Kari Alitalo

    Insert

    Tie1 (Tie1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    P hUbC

    Available since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTJK421

    Plasmid

    #138007

    Purpose

    BirA*-SIX2 expression. Vector also expresses GFP under control of hUBC promoter

    Depositor

    Tami Kingsbury

    Insert

    BirA* tagged SIX2 (SIX2 Human)
    

    Use

    Lentiviral

    Tags

    BirA*

    Expression

    Mutation

    None

    Promoter

    Available since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge