We narrowed to 22,137 results for: his

1. pDEST17-His10-ccdB-SNAP

   Plasmid

   #166146

   Purpose

   Gateway destination vector for generation of N-terminal His10-tagged and C-terminal SNAP-tagged proteins in E. coli.

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Type

   Empty backbone

   Use

   Tags

   His10 and SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDEST17-His10-Smt3-ccdB

   Plasmid

   #166147

   Purpose

   Gateway destination vector for generation of N-terminal His10-Smt3 tagged proteins in E. coli.

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Type

   Empty backbone

   Use

   Tags

   His10-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDEST17-His10-DDX5(kf)-SNAP

   Plasmid

   #166152

   Purpose

   Expression of N. furzeri DDX5 in E. coli

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Insert

   DDX5
   

   Use

   Tags

   His10 and SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNIC-HIS10-ZBASIC-TEV-GG

   Plasmid

   #215797

   Purpose

   Cloning of target gene with N-terminal His10 Zbasic tag and TEV cleavage

   Depositor

   Frank von Delft, ASAP Discovery

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pNIC-HIS6-SUMO1-AVITAG-GG

   Plasmid

   #215808

   Purpose

   Cloning of target gene with N terminal His6 SUMO2 tag and SENP1 cleavage non cleavable N-terminal avitag

   Depositor

   Frank von Delft, ASAP Discovery

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pFastBac1-His6-E3(1)-CUbo-TwinStrep

   Plasmid

   #212712

   Purpose

   Expression of His6-E3(1)-CUbo-TwinStrep in insect cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   E3(1)
   

   Use

   Tags

   6xHis and CUbo-TwinStrep

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET-6xHis-GFP(-6)-H2_mCherry(-6)

   Plasmid

   #205049

   Purpose

   Co-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coli

   Depositor

   Allie Obermeyer

   Article

   Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

   Insert

   GFP(-6)-H2_mCherry(-6)
   

   Use

   Tags

   MGHHHHHGGA

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET-6xHis-GFP(-6)-H2_mCherry(-6)-L1

   Plasmid

   #205050

   Purpose

   Co-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coli

   Depositor

   Allie Obermeyer

   Article

   Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

   Insert

   GFP(-6)-H2_mCherry(-6)-L1
   

   Use

   Tags

   GRRGKKSRK and MGHHHHHGGA

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET-6xHis-GFP(-6)-H2_mCherry(-6)-L2

   Plasmid

   #205051

   Purpose

   Co-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coli

   Depositor

   Allie Obermeyer

   Article

   Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

   Insert

   GFP(-6)-H2_mCherry(-6)-L2
   

   Use

   Tags

   GRRGKKSRKGRRGKKSRK and MGHHHHHGGA

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-6xHis-GFP(-6)-H2_mCherry(-6)-L3

    Plasmid

    #205052

    Purpose

    Co-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coli

    Depositor

    Allie Obermeyer

    Article

    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

    Insert

    GFP(-6)-H2_mCherry(-6)-L3
    

    Use

    Tags

    GRRGKKSRKGRRGKKSRKGRRGKKSRK and MGHHHHHGGA

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHyg-CUbo(K48R)-His6

    Plasmid

    #212778

    Purpose

    C-terminal PCR-based tagging with CUbo(K48R)-His6 in budding yeast with HygR selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHyg-CUbo(K63R)-His6

    Plasmid

    #212779

    Purpose

    C-terminal PCR-based tagging with CUbo(K63R)-His6 in budding yeast with HygR selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHyg-CUbo-yeGFP-His6

    Plasmid

    #212782

    Purpose

    C-terminal PCR-based tagging with CUbo-yeGFP-His6 in budding yeast with HygR selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRSF-Duet-C17orf53-6His

    Plasmid

    #211417

    Purpose

    bacterial expression of human C17orf53 C-terminally fused to 6xHis-Tag

    Depositor

    Helmut Pospiech

    Insert

    C17orf53 (HROB Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRSF-Duet-6His-C17orf53

    Plasmid

    #211416

    Purpose

    bacterial expression of human C17orf53 N-terminally fused to a 6xHis-Tag

    Depositor

    Helmut Pospiech

    Insert

    C17orf53 (HROB Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUra3-pCUP1-His7-NUbo

    Plasmid

    #212761

    Purpose

    N-terminal PCR-based tagging with pCUP1-His7-NUbo in budding yeast with URA3 selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET24a-His6-CUbo-MCS

    Plasmid

    #212725

    Purpose

    Bacterial expression of a protein of interest with N-terminal His6-CUbo-fusion, contains multiple cloning site

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET24a-His7-NUbo-MCS

    Plasmid

    #212724

    Purpose

    Bacterial expression of a protein of interest with N-terminal His7-NUbo-fusion, contains multiple cloning site

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET24a-NUbo-MCS-His6

    Plasmid

    #212723

    Purpose

    Bacterial expression of a protein of interest with N-terminal NUbo- and C-terminal His6-fusions, contains multiple cloning site

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET15b-His6-CUbo-GFP

    Plasmid

    #212720

    Purpose

    Bacterial expression of His6-CUbo-GFP

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    GFP
    

    Use

    Tags

    6xHis-CUbo

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET24a-His7-NUbo-GFP

    Plasmid

    #212719

    Purpose

    Bacterial expression of His7-NUbo-GFP

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    GFP
    

    Use

    Tags

    7xHis-NUbo

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET24a-NUbo-GFP-His6

    Plasmid

    #212718

    Purpose

    Bacterial expression of NUbo-GFP-His6

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    GFP
    

    Use

    Tags

    6xHis and NUbo

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET30-His6-TEV-Mms2

    Plasmid

    #212716

    Purpose

    Bacterial expression of His6-Mms2, His6-tag cleavable with TEV-protease

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    Mms2
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMAL-TEV2-Ubc7-His6

    Plasmid

    #212713

    Purpose

    Bacterial expression of MBP-Ubc7-His6, MBP-tag cleavable with TEV-protease

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    Ubc7
    

    Use

    Tags

    6xHis and MBP-TEV2

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pETM11-6xHis-TEV-hORF1p_R261A

    Plasmid

    #202586

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the R261A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    ORF1p
    

    Use

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Changed ORF1p Arginine 261 to Alanine

    Promoter

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pETM11-6xHis-TEV-hORF1p_StammerAAA

    Plasmid

    #202587

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A, E92A, and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    ORF1p
    

    Use

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…

    Promoter

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pETM11-6xHis-TEV-hORF1p_StammerAEA

    Plasmid

    #202588

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    ORF1p
    

    Use

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Changed ORF1p Methionine 91 to Alanine, changed O…

    Promoter

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJRM919_WT aa 1-419 His POLI

    Plasmid

    #201445

    Purpose

    Low copy vector for expressing the catalytic domain (amino acids 1-419) of human DNA polymerase iota in E. coli with an N-terminal 6x His tag. C-terminal truncation mutant of pJRM919_WT His POLI

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Expresses amino acids 1-449 of DNA polymerase iot…

    Promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PET-HypaSpCas9-NLS-6xHis

    Plasmid

    #207384

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HypaSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N692A, M694A, Q695A, H698A

    Promoter

    T7

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GB1-His-TEV-A7 (2-180)

    Plasmid

    #190066

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain.

    Depositor

    Lalit Deshmukh

    Article

    Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

    Insert

    Annexin A7 PRD (ANXA7 Human)
    

    Use

    Tags

    GB1-His-TEV

    Expression

    Bacterial

    Mutation

    PRD only, residues 2-180

    Promoter

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBAD-HisD-mRhubarb713-HO1

    Plasmid

    #197254

    Purpose

    Expresses the protein of mRhubarb713 and HO1 in bacteria

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    mRhubarb713
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PET-B-HeFSpCas9-NLS-6xHis

    Plasmid

    #207392

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A…

    Promoter

    T7

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PET-HeFSpCas9-NLS-6xHis

    Plasmid

    #207391

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HeFSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

    Promoter

    T7

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. PET-evoSpCas9-NLS-6xHis

    Plasmid

    #207389

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    evoSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M495V, Y515N, K526E, R661Q

    Promoter

    T7

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDL25_Cas9-His Δcys E1207C

    Plasmid

    #206286

    Purpose

    Bacterial expression of Cas9-His Δcys E1027C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    C80S, C574S, E1207C

    Promoter

    T7

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDL23_Cas9-His Δcys E945C

    Plasmid

    #206284

    Purpose

    Bacterial expression of Cas9-His Δcys E945C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    C80S, C574S, E945C

    Promoter

    T7

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDL24_Cas9-His Δcys E1026C

    Plasmid

    #206285

    Purpose

    Bacterial expression of Cas9-His Δcys E1026C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    C80S, C574S, E1026C

    Promoter

    T7

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDL22_Cas9-His Δcys E532C

    Plasmid

    #206283

    Purpose

    Bacterial expression of Cas9-His Δcys E532C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    C80S, E532C, C574S

    Promoter

    T7

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. His-ATG3-K62D/K64D

    Plasmid

    #202786

    Purpose

    ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-K62D/K64D-stop

    Depositor

    James Hurley

    Article

    Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

    Insert

    ATG3 (ATG3 Human)
    

    Use

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Mutation

    K62D, K64D

    Promoter

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET24-aCatenin(82-906)-His6

    Plasmid

    #203332

    Purpose

    Bacterial expression of wild-type alpha-catenin

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2012 May 25;287(22):18492-9

    Insert

    CTNNA1 (CTNNA1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 82-906

    Promoter

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge