We narrowed to 16,181 results for: nes

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pLenti-EF-FH-TAZ-ires-blast

Plasmid

#52083

Purpose

Lentiviral expression vector for TAZ with N-terminal FLAG and His tags

Depositor

Yutaka Hata

Publication

Yang et al Mol Cell Biol. 2014 Feb 18.

Insert

TAZ (WWTR1 Human)

Use

Lentiviral

Tags

FLAG and His

Expression

Mammalian

Promoter

EF1a

Available since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pBK-YE1-BE3

Plasmid

#85174

Purpose

Expresses YE1-BE3 in mammalian cells

Depositor

David Liu

Publication

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

YE1-BE3

Expression

Mammalian

Promoter

CMV

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pJSC173 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

Plasmid

#101218

Purpose

Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

Depositor

Jennifer Doudna, Keith Joung

Publication

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant N692A/M694A/Q695A/H698A

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

N692A, M694A, Q695A and H698A

Promoter

T7

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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DP6

Plasmid

#140446

Purpose

Untargetted bacterial drift

Depositor

David Liu

Publication

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 dam seqA emrR ugi CDA1 gIII

Expression

Bacterial

Available since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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PresentER-SIINFEKL (GFP)

Plasmid

#102944

Purpose

PresentER minigene encoding H2-Kb ligand SIINFEKL (GFP)

Depositor

David Scheinberg

Publication

Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

Insert

SIINFEKL (OVAL Synthetic, Chicken)

Use

Retroviral

Expression

Mammalian

Mutation

OVA257-264

Promoter

LTR

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pU6-Universal

Plasmid

#52694

Purpose

This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.

Depositor

Sebastian Lourido

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Cas9
Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA

Use

CRISPR; Toxoplasma gondii

Tags

3X FLAG and Nuclear localization signal

Promoter

TgTUB1 and Toxoplasma U6

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

34 Articles

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pmBanana-Hyg-CTCF-NtermNES-N1

Plasmid

#131850

Purpose

Expresses the [N-terminus of human CTCF (a.a. 2-265)]-mBanana with a nuclear export signal in mammalian cells

Depositor

Mark Minden

Publication

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

CTCF (CTCF Human)

Tags

mBanana

Expression

Mammalian

Mutation

deleted amino acids 796-2181 (the N-terminus is l…

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-RhoA Biosensor

Plasmid

#68026

Purpose

C-terminus of Anillin in mammalian expression vector pEGFP-C1

Depositor

Michael Glotzer

Publication

Piekny et al Curr Biol. 2008 Jan 8;18(1):30-6. Epub 2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Anillin (AHD+PH) (ANLN Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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PKC iota WT

Plasmid

#21252

Depositor

Bernard Weinstein

Publication

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PKC iota (PRKCI Human)

Tags

HA

Expression

Mammalian

Available since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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ALKBH5-dCas9

Plasmid

#134783

Purpose

Expression fusion protein ALKBH5-dCas9 in Mammalian cells

Depositor

Shu-Bing Qian

Publication

Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ALKBH5 and inactive dCas9

Expression

Mammalian

Promoter

CMV

Available since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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Ef1a-pspCas13b-NES-3xFLAG-T2A-BFP

Plasmid

#173029

Purpose

For expression of cytoplasmic pspCas13b protein tagged with 3xHA-T2A-BFP for gene silencing in mamallian cells.

Depositor

Mohamed Fareh

Publication

Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

Insert

pspCas13b

Use

CRISPR and Lentiviral

Tags

3xFLAG, BFP, HIV NES, and T2A

Expression

Mammalian

Promoter

Ef1a

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-FLAG PTEN

Plasmid

#78777

Purpose

To overexpress PTEN in Mammalian Cells

Depositor

Jaewhan Song

Publication

Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

Insert

PTEN (PTEN Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pBI-p53 wt/EGFP

Plasmid

#16543

Depositor

Bert Vogelstein

Publication

Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

p53 (TP53 Human)

Expression

Mammalian

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pVS1

Plasmid

#33143

Depositor

Michael Rosbash

Publication

Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.

Insert

LacO (256 copies)

Expression

Yeast

Available since

Dec. 20, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
PiggyBac dCas9-VPR

Plasmid

#191269

Purpose

piggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFP

Depositor

Joanna Wysocka

Publication

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

VPR-dCas9-HA-tagBFP
rtTA3-IRES-PURO

Use

CRISPR

Tags

HA and tagBFP

Expression

Mammalian

Available since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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EBFP-C1

Plasmid

#54738

Purpose

Localization: C1 Cloning Vector, Excitation: 387, Emission: 450

Depositor

Michael Davidson

Type

Empty backbone

Tags

EBFP

Expression

Mammalian

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pAR-Ec633

Plasmid

#130873

Purpose

A nuclear plasmid encoding an error-prone mutant TP-DNAP1 (L477V, L640Y, I777K, W814N) for OrthoRep

Depositor

Chang Liu

Publication

Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

Insert

TP-DNAP1

Expression

Yeast

Mutation

L477V, L640Y, I777K, W814N

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDY1173_ADAR1p150

Plasmid

#193192

Purpose

Express full length ADAR1p150

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ADAR1p150 (ADAR Human)

Expression

Mammalian

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRCC-K

Plasmid

#81191

Purpose

Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance

Depositor

Eckhard Boles

Publication

Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

Inserts

Cas9
empty gRNA cassette

Use

CRISPR

Tags

NTS

Expression

Yeast

Mutation

Please see depositor comments below. and WT - cod…

Promoter

ROX3 and SNP52p

Available since

Sept. 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMCS.DT-A

Plasmid

#22730

Depositor

Mark Magnuson

Publication

Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

Insert

DT-A

Use

Bac recombineering

Mutation

This is a gap-repair vector designed for retrievi…

Available since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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