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SPACE-NG (pRZ5150)

Plasmid

#140244

Purpose

CMV promoter expression plasmid for TadA*(V82G)-nCas9_NG-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

SPACE_NG

Expression

Mammalian

Mutation

V82G in TadA*, NG mutations in SpCas9, D10A in Sp…

Promoter

CMV

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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pLVUTHshGATA1-tTR-KRAB

Plasmid

#11650

Purpose

Tet-regulated (Tet-on) lentiviral vector for shGATA1 (hUbiquitin promoter) - 3rd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

hUbiquitin C, GFP, tTR-KRAB, shRNA against GATA1, Tet-on (GATA1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090

Plasmid

#103871

Purpose

dPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to selectively edit adenosine to inosine in RNA when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Inserts

PspCas13b
huADAR2DD (ADARB1 Human)

Use

CRISPR

Tags

GS and HIV NES

Expression

Mammalian

Mutation

Changed histidne 133 to alanine and histidine 105…

Available Since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-14-3-3 gamma

Plasmid

#13274

Depositor

Michael Yaffe

Insert

14-3-3 gamma (Ywhag Rat)

Tags

HA

Expression

Mammalian

Available Since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

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UbC-TRE-mSOD2-tTS-IRES-EGFP

Plasmid

#12390

Depositor

Zuoshang Xu

Article

Xia et al Biotechniques. 2006 Jul . 41(1):64-8.

Insert

mSOD2 shRNA (Sod2 Mouse)

Use

RNAi

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

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MEK1-GFP

Plasmid

#14746

Depositor

Rony Seger

Article

Yao et al Oncogene. 2001 Nov 15. 20(52):7588-96.

Insert

MEK1 (MAP2K1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 12, 2007

Availability

Academic Institutions and Nonprofits only

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pWSK29

Plasmid

#172972

Purpose

Low copy number cloning vector

Depositor

Renato Morona

Article

Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pHR_XPR2_hrGFP

Plasmid

#84614

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into XPR2 locus

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pJYS1Peftu

Plasmid

#85546

Purpose

Constitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter

Depositor

Sheng Yang

Article

Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

Inserts

Cpf1
recT

Use

CRISPR; Shuttle vector corynebacterium glutamicum…

Expression

Bacterial

Promoter

etfu and unknown

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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Flag-SENP1

Plasmid

#17357

Depositor

Edward Yeh

Article

Cheng et al Cell. 2007 Nov 2. 131(3):584-95.

Insert

SENP1 (SENP1 Human)

Tags

Flag

Expression

Mammalian

Available Since

March 19, 2008

Availability

Academic Institutions and Nonprofits only

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