We narrowed to 1,947 results for: 974

1. pEBB COMMD5 Flag

   Plasmid

   #74897

   Purpose

   Express COMMD5 in mammalian cells

   Depositor

   Ezra Burstein, Colin Duckett

   Article

   Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

   Insert

   COMMD5 (COMMD5 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha promoter

   Available since

   June 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYLL204

   Plasmid

   #53455

   Purpose

   Expresses active site tyrosine mutated HA-tagged mouse DNA topoisomerase IIbeta Y814F (full length cDNA, in pCS2+ backbone)

   Depositor

   Lisa Lyu, Leroy Liu

   Article

   Ban et al Mol Cell Biol. 2013 Oct;33(20):4008-16.

   Insert

   HA-tagged Mus musculus topoisomerase (DNA) II beta (Top2b) Y814F (Top2b Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Y814F

   Promoter

   CMV

   Available since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1

   Plasmid

   #98972

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 1 and Cas9(D10A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2

   Plasmid

   #98973

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 2 and Cas9(D10A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRGEB-VirD2-Cas9

   Plasmid

   #139803

   Purpose

   Expressing VirD-Cas9 fusion under ubiquitin promoter

   Depositor

   Magdy Mahfouz

   Article

   Ali et al Commun Biol. 2020 Jan 23;3(1):44. doi: 1

   Insert

   VirD2-Cas9
   

   Use

   Tags

   VirD2-Cas9

   Expression

   Plant

   Mutation

   Promoter

   Ubiquitin

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. mKO-Mito-7

   Plasmid

   #57848

   Purpose

   Localization: Mitochondria, Excitation: 548, Emission: 561

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   Mito (COX8A Human)
   

   Use

   Tags

   mKO

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFETCh_FOXP1

   Plasmid

   #86261

   Purpose

   Donor vector for 3' FLAG tag of human FOXP1

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   FOXP1 homology arms (FOXP1 Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. GST-GAR

   Plasmid

   #34697

   Depositor

   Steven Clarke

   Article

   Frankel et al J Biol Chem. 2000 Oct 20;275(42):32974-8

   Insert

   Fibrillarin (FBL Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Residues 1-145 of human fibrillarin with K2E and …

   Promoter

   tac

   Available since

   July 12, 2012

   Availability

   Academic Institutions and Nonprofits only

9. psgRNA (GB0645)

   Plasmid

   #68210

   Purpose

   Provides a Short guide RNA which is the combination of the bacterial crRNA and tracrRNA into a single guide transcript; it does not contain any target site

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   SgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5 FRT TO myc Separase C2029A

    Plasmid

    #59822

    Purpose

    Allows the integration of myc Separase C2029A in the genome and Tet-inducible expression

    Depositor

    Stephen Taylor

    Article

    Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

    Insert

    Separase (ESPL1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    C2029A

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. PX458_FOXP1_1

    Plasmid

    #86299

    Purpose

    Encodes gRNA for 3' target of human FOXP1

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against FOXP1 (FOXP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-rtTA-zeo

    Plasmid

    #183233

    Purpose

    Lentiviral vector expressing the rtTa gene

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Insert

    rtTA-T2A-Zeo
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    UBC

    Available since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTS115 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-Alfa-P2A-TagBFP

    Plasmid

    #199368

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-SUMOstar-ALFA-P2A-TagBFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. LRP6 dC-pCS2

    Plasmid

    #27258

    Depositor

    Xi He

    Article

    Tamai et al Nature. 2000 Sep 28. 407(6803):530-5.

    Insert

    low density lipoprotein receptor-related protein 6 (LRP6 Human)
    

    Use

    Xenopus injection

    Tags

    Expression

    Mammalian

    Mutation

    carboxyl intracellular region deleted Residue 14…

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pCIneo-V5-SBP-HseIF4AII_AC

    Plasmid

    #148439

    Purpose

    Mammalian Expression of HseIF4AII

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    HseIF4AII (EIF4A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. PX458_FOXP1_2

    Plasmid

    #86300

    Purpose

    Encodes gRNA for 3' target of human FOXP1

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against FOXP1 (FOXP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223 GABARAP G116A

    Plasmid

    #123081

    Purpose

    Gateway entry clone encoding human GABARAP G116A

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)
    

    Use

    Gateway entry vector / entry clone

    Tags

    Expression

    Mutation

    Glycine 116 to Alanine

    Promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. mWasabi-Mito-7

    Plasmid

    #56508

    Purpose

    Localization: Mitochondria, Excitation: 493, Emission: 509

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    mWasabi

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pLJC6-Flag CASTOR1

    Plasmid

    #84496

    Purpose

    Lentiviral expression of CASTOR1

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

    Insert

    CASTOR1 (GATSL3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    sestrin2

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pFastBac-CHD7 (1-1899)

    Plasmid

    #170143

    Purpose

    Expresses residues 1-1899 of CHD7 in insect cells

    Depositor

    Robert Kingston

    Article

    Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

    Insert

    chromodomain helicase DNA binding protein 7 (CHD7 Human)
    

    Use

    Tags

    FLAG

    Expression

    Insect

    Mutation

    C-terminal truncation of residues 1900-2997

    Promoter

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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