We narrowed to 23,178 results for: Nov
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pODN-mNG-RHOA
Plasmid#183836PurposeRepair template for the N-terminal tagging of RhoA with mNeonGreen in human cells using CRISPR/Cas9.DepositorInsertRHOA homology arms with mNeonGreen-linker (RHOA Human)
UseCRISPR; Donor templateTagsmNeonGreen-linkerExpressionMammalianMutationHomology arms contain point mutations to remove t…PromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCX0393
Plasmid#205984PurposeGa0116245_10002768 T7/LacO-Cas6 + Cas14-TwinStrep + Cas7 + His14-Cas5::pJ23119-truncated DR-truncated Fn spacerDepositorInsertsGa0116245_10002768 candidate type VII proteins
Cas14
Cas5
truncated CRISPR array
UseTags14xHis and TwinStrep-tagExpressionBacterialMutationPromoterT7 (proteins), pJ23119 (crRNA)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1950
Plasmid#205983PurposeGa0116245_10002768 T7/LacO-Cas6 + TwinStrep-Cas14 + Cas7 + His14-Cas5::pJ23119-DR-Fn spacer-DRDepositorInsertsGa0116245_10002768 candidate type VII proteins
Cas14
Cas5
CRISPR array
UseTags14xHis and TwinStrep-tagExpressionBacterialMutationPromoterT7 (proteins), pJ23119 (crRNA)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
2xmyc-RAB10
Plasmid#164631PurposeExpresses RAB10 in mammalian cells with a 2xmyc tagDepositorInsertRas-related protein Rab-10 (RAB10 Human)
UseTags2xmycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
H2B-mAvicFP1
Plasmid#129511PurposeExpresses H2B-mAvicFP1 in mammalian cells (human histone 2B)DepositorInsertH2B-mAvicFP1
UseTagsExpressionMammalianMutationmAvicFP1 fused to the C terminus of human histone…PromoterCMVAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB80-hsKIF5B(1-560)Rigor-L-2xHA
Plasmid#193718PurposeExpression of HA tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubulesDepositorInserthsKIF5B(1-560)Rigor-L-2xHA (KIF5B Synthetic, Human)
UseTags2xHAExpressionMammalianMutationhsKIF5B(aa1-560), RIGOR: G234APromoterChicken beta-actinAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-IRES-blasticidin-Sox2 WT
Plasmid#182046Purposeexpression of Sox2DepositorInsertSox2 (Sox2 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCAGAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shOrai1
Plasmid#89933PurposeKnockdown human Orai1 expressionDepositorInsertOrai1 shRNA (ORAI1 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_eGFP::Dpp
Plasmid#163702PurposeGFP-tagged version of Dpp (Decapentaplegic) under control of UAS and LexO enhancersDepositorInsertdecapentaplegic (dpp Fly)
UseCre/LoxTagsTagged with eGFPExpressionInsectMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-STIM1
Plasmid#89817PurposeExpress HUMAN STIM1DepositorInsertSTIM1 (STIM1 Human)
UseRetroviralTagsNO TAGExpressionMutationPromoterAvailable sinceJune 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHS1859
Plasmid#205972PurposeFLAG-SV40 NLS-Csy3/Cas7 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)DepositorInsertCsy3/Cas7 from JAGBLH010000220, Cas8-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1860
Plasmid#205973PurposeFLAG-SV40 NLS-Cas6/Csy4 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)DepositorInsertCas6/Csy4 from JAGBLH010000220, Cas8-HNH
UseTagsFLAG-SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pODN-mR2-ACTB
Plasmid#183868PurposeRepair template for the N-terminal tagging of beta-actin with mRuby2 in human cells using CRISPR/Cas9.DepositorInsertACTB homology arms with mRuby2-linker (ACTB Human)
UseCRISPR; Donor templateTagsmRuby2-linkerExpressionMammalianMutationHomology arms contain point mutations to remove t…PromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNCST-mAvicFP1
Plasmid#129509PurposeExpresses mAvicFP1 constitutively in E. coli (most strains)DepositorInsertmAvicFP1
UseTagsExpressionBacterialMutationPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-hCD2-CD14 (4R)
Plasmid#133062PurposeRetroviral expression of mouse CD14 mutant "4R" deficient in LPS bindingDepositorInsertCD14 (4R) (Cd14 Mouse)
UseRetroviralTagsExpressionMammalianMutationCD14 allele defective for LPS binding (mutations …PromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pODN-mNG-ECT2
Plasmid#183835PurposeRepair template for the N-terminal tagging of Ect2 with mNeonGreen in human cells using CRISPR/Cas9.DepositorInsertECT2 homology arms with mNeonGreen-linker (ECT2 Human)
UseCRISPR; Donor templateTagsmNeonGreen-linkerExpressionMammalianMutationHomology arms contain point mutations to remove t…PromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shSTIM1
Plasmid#89816PurposeKnockdown human STIM1 expressionDepositorInsertSTIM1 shRNA (STIM1 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHS1801
Plasmid#205966PurposeJAGBLH010000220 Cas8-HNH full system (E. coli codon optimized) in pACYCDuet-1 with Lac promotersDepositorInsertCas8-HNH proteins and CRISPR array
UseTagsExpressionBacterialMutationPromoterLac (proteins), pJ23119 (crRNA)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHS1232
Plasmid#205969PurposeMWEE01000013 Cas5-HNH operon (E. coli codon optimized) + CRISPR array in pACYCDuet-1 Lac promotersDepositorInsertCas5-HNH proteins and CRISPR array
UseTagsExpressionBacterialMutationPromoterLac (proteins), pJ23119 (crRNA)Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only
