We narrowed to 3,081 results for: eed

1. LV-AR-C563A-C596A

   Plasmid

   #171220

   Purpose

   Lenti-viral expression of human androgen receptor DNA-binding mutant: mutations of one essential cysteine on each of the two zinc fingers.

   Depositor

   Steven Balk

   Article

   Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

   Insert

   AR-C563A-C596A (AR Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   AR-C563A-C596A

   Promoter

   EF-1a

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBIG1b_10xHisTEVATG5_ATG12

   Plasmid

   #190861

   Purpose

   Plasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178

   Depositor

   Sascha Martens

   Article

   Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

   Inserts

   ATG5 (ATG5 Human)
   ATG12 (ATG12 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR ErbB2-CD3ε-FusionRed

   Plasmid

   #188628

   Purpose

   Human ErbB2 labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensor

   Depositor

   Jared Toettcher

   Article

   Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

   Insert

   ERBB2 (ERBB2 Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   CD3ε-FusionRed

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available Since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. LV-AR-R599A-N600A

   Plasmid

   #171221

   Purpose

   Lenti-viral expression of human androgen receptor DNA-binding mutant: R599A-N600A, mutations in the D-box of the Zinc finger 2 that mediates AR dimerization.

   Depositor

   Steven Balk

   Article

   Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

   Insert

   AR-R599A-N600A (AR Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   AR-R599A-N600A

   Promoter

   EF-1a

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST-ERBB3-v1

   Plasmid

   #154893

   Purpose

   Expresses ERBB3 (a.k.a HER3) fused to Venus fragment 1 (v1) for use in bimolecular fluorescence complementation (BiFC) assays

   Depositor

   Johanna Ivaska

   Article

   Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

   Insert

   ERBB3 (ERBB3 Human)
   

   Use

   Tags

   Venus fragment 1 (v1)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. LV-AR-AAFF

   Plasmid

   #171218

   Purpose

   Lenti-viral expression of human androgen receptor DNA-binding mutant: K580A-V581A, mutations in the P-box of the zinc finger 1 at the KVFF motif.

   Depositor

   Steven Balk

   Article

   Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

   Insert

   AR-AAFF (AR Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   AR-K580A-V581A

   Promoter

   EF-1a

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXS-IRES-Blast 3XFLAG-CHP1

   Plasmid

   #173166

   Purpose

   Retroviral vector to express sgRNA resistant 3XFLAG tagged human CHP1

   Depositor

   Kivanc Birsoy

   Article

   Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

   Insert

   Calcineurin Like EF-Hand Protein 1 (CHP1 Human)
   

   Use

   Retroviral

   Tags

   3XFLAG

   Expression

   Mutation

   Synonymous mutations at sgRNA sites

   Promoter

   MMLV

   Available Since

   Aug. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCV Cdk2ap1CAN-HA

   Plasmid

   #178030

   Purpose

   Retroviral vector for the purpose of overexpression in mammalian cell culture

   Depositor

   Lin He

   Article

   Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

   Insert

   Cdk2ap1 (Cdk2ap1 Mouse)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   First N-Terminal 27aa are deleted

   Promoter

   GAG

   Available Since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSB65 - pL0_tGFP-I (CDS1)

   Plasmid

   #123182

   Purpose

   Golden Gate (MoClo; CDS1) compatible turbo GFP gene from P. plumata with an intron for transient and stable expression in plants (moved to a CDS1 position from pICSL50016; Engler et al., 2014)

   Depositor

   Carolyn Lee-Parsons, Erin Cram

   Article

   Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

   Insert

   turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A. thaliana)
   

   Use

   Part for plant expression

   Tags

   Expression

   Plant

   Mutation

   No BpiI and BsaI sites; codon-optimised for plants

   Promoter

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX459-dCTNNA1

    Plasmid

    #176032

    Purpose

    A knock-out vector for dog CTNNA1 (alpha-1-catenin)

    Depositor

    Michiyuki Matsuda

    Article

    Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

    Insert

    A gRNA targeting the dog CTNNA1 (alpha-1-catenin) gene and the cDNA of Cas9 (CTNNA1 )
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSF_his6_sumo_ZIKV_NS5_MTase

    Plasmid

    #213529

    Purpose

    Expresses the MTase domain of NS5 of ZIKV

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    ZIKA virus NS5 methyltransferase domain
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    MTase domain aa 1-265

    Promoter

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-RepoMan-wt

    Plasmid

    #184269

    Purpose

    Expresses shRNA resistant GFP-RepoMan

    Depositor

    Lienhard Schmitz

    Article

    Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

    Insert

    cell division cycle associated 2 (CDCA2 Human)
    

    Use

    Tags

    3xHA and GFP

    Expression

    Mammalian

    Mutation

    changed nucleotides 723-747, 1488-1509, 1524-1548…

    Promoter

    CMV

    Available Since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CMV-FLEX-SaCas9-U6-sgFAAH

    Plasmid

    #209197

    Purpose

    Mutagenesis of Faah

    Depositor

    Larry Zweifel

    Article

    Balsevich et al Elife. 2023 Apr 11;12:e81919. doi: 10.75

    Insert

    Faah (Faah Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCV Cdk2ap1ΔN (MT2B2)-HA

    Plasmid

    #178031

    Purpose

    Retroviral vector for the purpose of overexpression in mammalian cell culture

    Depositor

    Lin He

    Article

    Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

    Insert

    Cdk2ap1 (Cdk2ap1 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    First N-Terminal 27aa are deleted

    Promoter

    GAG

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX459-dMET

    Plasmid

    #176031

    Purpose

    A knock-out vector for dog MET

    Depositor

    Michiyuki Matsuda

    Article

    Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

    Insert

    A gRNA targeting the dog MET gene and the cDNA of Cas9 (MET )
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMXS-IRES-Blast ACSL3

    Plasmid

    #173165

    Purpose

    Retroviral vector to express sgRNA resistant human ACSL3

    Depositor

    Kivanc Birsoy

    Article

    Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

    Insert

    Acyl-CoA Synthetase Long Chain Family Member 3 (ACSL3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Synonymous mutations at sgRNA sites

    Promoter

    MMLV

    Available Since

    Aug. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSF_his6_sumo_SARS_CoV2_NSP14_D90A/E92A

    Plasmid

    #213527

    Purpose

    Expresses NSP14 of SARS-CoV-2 with two point mutations D90A and E92A

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    NSP14 of SARS-CoV-2 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    D90A and E92A

    Promoter

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSF_his6_sumo_OC43_NSP14

    Plasmid

    #213519

    Purpose

    Expresses NSP14 of hCoV OC43

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    hCoV OC43 NSP14 (ORF1ab hCoV OC43)
    

    Use

    Tags

    His-SUMO tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSF_his6_sumo_NL63_NSP14

    Plasmid

    #213520

    Purpose

    Expresses NSP14 of hCoV NL63

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    hCoV NL63 NSP14 (ORF1ab hCoV NL63)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSF_his6_sumo_229E_NSP14

    Plasmid

    #213521

    Purpose

    Expresses NSP14 of hCoV 229E

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    hCoV 229E NSP14
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge