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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,800 results for: emb

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Showing: 1281 - 1300 of 11800 results

  1. mbYFPQS

    Plasmid
    #80742
    Purpose
    membrane-targeted biosensor for monitoring changes in intracellular chloride
    Depositor
    Susan Amara, Susan Ingram
    Publication
    Watts et al PLoS One. 2012;7(4):e35373. doi: 10.1371
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    YFPQS
    Tags
    palmitoylation signal sequence of neuromodulin
    Expression
    Mammalian
    Mutation
    H148Q (CAC to CAG) and V163S (GTG to AGC).
    Promoter
    CMV
    Available since
    Aug. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  2. Mucolipin1-pHcRed C1

    Plasmid
    #62959
    Purpose
    full length human Mucolipin-1 cloned into pHcRed1 C1
    Depositor
    Paul Luzio
    Publication
    Pryor et al Traffic. 2006 Oct;7(10):1388-98.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Mucolipin-1 (MCOLN1 Human)
    Tags
    HcRed1
    Expression
    Mammalian
    Mutation
    Mucolipin-1 has 1 silent mutation compared to the…
    Promoter
    CMV
    Available since
    May 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62959-plasmid-map-sequence-id-383949

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  4. CD154-His-bio

    Plasmid
    #52337
    Purpose
    Expresses full-length Tumor necrosis factor ligand superfamily member 5 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    CD154 (CD40LG Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52337-plasmid-map-sequence-id-81058

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  8. pCI H2B-GFP

    Plasmid
    #92399
    Purpose
    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-EGFP reporter.
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    IRES H2B EGFP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    92399-plasmid-map-sequence-id-178249

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  9. pCRISPReporter-mCherry

    Plasmid
    #65008
    Purpose
    E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with mCherry reporter.
    Depositor
    Mattheos Koffas
    Publication
    Cress et al ACS Synth Biol. 2015 Mar 30.
    Insert
    mCherry
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    User-defined
    Available since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    65008-plasmid-map-sequence-id-109895

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  11. pMA-SpCas9-g4

    Plasmid
    #80787
    Purpose
    BsaI-based cloning of SpCas9 gRNA guide sequence, position 4/14/24 in the array
    Depositor
    Yonglun Luo
    Publication
    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR gRNA expression cassette (for SpCas9)
    Use
    CRISPR
    Tags
    na
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    80787-plasmid-map-sequence-id-162407

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  12. NR4A1

    Plasmid
    #25477
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NR4A1 (NR4A1 Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  14. EB3N-VVDfast-Halo-iLID

    Plasmid
    #190166
    Purpose
    Expresses EB3N-VVDfast-Halo-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.
    Depositor
    Anna Akhmanova
    Publication
    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EB3N-VVDfast-Halo-iLID (MAPRE3 Human, Synthetic)
    Tags
    -VVDfast-mClover3-iLID
    Expression
    Mammalian
    Mutation
    1-189 aa from EB3
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    190166-plasmid-map-sequence-id-374286

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  19. LAMP-mChF-AIP

    Plasmid
    #61524
    Purpose
    Expression of Homo sapiens lysosomal-associated membrane protein 1 (LAMP1) tagged with mCherry and AIP
    Depositor
    Takanari Inoue
    Publication
    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    lysosomal-associated membrane protein 1 (LAMP1 Human)
    Tags
    AIP and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61524-plasmid-map-sequence-id-338156

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Showing: 1281 - 1300 of 11800 results