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Plasmid#184638PurposeFluorescent reporter for mammalian cells: Expression of mScarlet with SARS-CoV-2 5'UTRDepositorInsertmScarlet
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 2, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTol2-nbt-Electra2-P2A-mScarlet
Plasmid#196114Purposezebrafish expression of nbt-Electra2-P2A-mScarletDepositorInsertnbt-Electra2-P2A-mScarlet
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-LTR-dCas9-p65AD-BFP
Plasmid#46913PurposeHuman expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, p65 activation domain and tagBFPDepositorInsertsdCas9-p65AD-BFP fusion
Puromycin resistance
UseCRISPR and RetroviralTags2xNLS, BFP, and VP64 domainExpressionMammalianMutationPromoterLTR and PGKAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDB-hCRY1-mScarlet-I-CD4-bla
Plasmid#179442PurposeDonor vector to knock in mScarlet-I C-terminal to human CRY1 geneDepositorInsertmScarletI
UseCRISPRTagsHis/FlagExpressionMutationPromoterNoneAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV p2GM AMPK alpha2hp1 alpha1hp1
Plasmid#89492PurposeshRNA against AMPK alpha2 and alpha1 in mouse/human to knock down protein expressionDepositorInsert2 hairpins against mouse/human AMPKalpha1 and alpha2
UseRetroviralTagsExpressionMammalianMutationPromoterU6 (The GFP-Puro is from a PGK promoter; the shRN…Available sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
ROPY (sYFP2-OP18-mScI-R125I)
Plasmid#128002PurposeImproved redshifted FRET-readout of Stathmin/OP18 interaction with α-tubulinDepositorInsertsYFP2-OP18-mScI-R125I
UseTagsExpressionMammalianMutationR125I in mScarlet-IPromoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mScarlet-TEV-Strep-His
Plasmid#210823PurposeBacterial overexpression vector for C-terminally Strep and His tagged mScarlet fluorescent proteinDepositorInsertmScarlet
UseTagsTwinStrep, 10xHisExpressionBacterialMutationPromoterT7Available sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSCV-mouse Ezh2-IRES-GFP
Plasmid#107146PurposeMammalian expression of mouse Ezh2DepositorInsertEzh2 (Ezh2 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP/CALR ins5
Plasmid#214701PurposeMammalian expression of human CALR ins5DepositorInsertCALR ins5 (CALR Human)
UseRetroviralTagsExpressionMutationPromoterMSCVAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP/CALR del52
Plasmid#214700PurposeMammalian expression of human CALR del52DepositorInsertCALR (CALR Human)
UseRetroviralTagsExpressionMutationPromoterMSCVAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-NLS-CBGreen-FIRE-puro
Plasmid#139195PurposeLuminescent reporter of ERK activityDepositorInsertCBG99
UseLuciferase and RetroviralTagsFra1 PEST domain fragment (FIRE) and nuclear loca…ExpressionMammalianMutationSee depositor comments belowPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-mouse p19ARF-IRES-GFP
Plasmid#107147PurposeMammalian expression of mouse ArfDepositorInsertArf [alternative reading frame] (Cdkn2a Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpRY-P2A-mScarlet (MNW002)
Plasmid#174137PurposeCMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarletDepositorInserthuman codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-mScarletExpressionMammalianMutationSpG=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317…PromoterCMV and T7Available sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_mitoLAMA-F98 IRES ShadowG-mScarlet
Plasmid#130708PurposePlasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and ShadowG-mScarletDepositorInsertNTOM20-SNAP-Tag-LAMA-F98 IRES ShadowG-mScarlet
UseTagsNTOM20 and SNAP-TagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDSP15 (5’Δ HygR + loxP + MCS + 5’Δ mScarlet-I)
Plasmid#193853PurposePromoterless insertion vector for split HygR/mScarlet-I landing padDepositorInsert5' rps-0::HygR + 5' mScarlet-I
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterrps-0pAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRRL-mU6-GFPGO2-IRES-mScarletI
Plasmid#136896PurposeLentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO.DepositorInsertGFPGO2-IRES-mScarletI
UseLentiviralTags2xNLS on GFP, 3xNLS on mScarletExpressionMutationATG>ACGPromoterSFFVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV--Mouse Mdm2 wt-IRES-CFP
Plasmid#180493PurposeRole of Mdm2 splicing variants in tumorigenesisDepositorInsertMdm2 full length (wt)
UseRetroviralTagsExpressionMammalianMutationPromoterMSCVAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
p6642 MSCV-P C-FlagHA 6bE7-Kozak
Plasmid#35020DepositorInsertHPV6b E7 (E7 HPV 6b)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterMSCV LTRAvailable sinceMarch 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-ChRmine-mScarlet-WPRE
Plasmid#130990PurposeAAV vector to drive the expression of ChRmine-mScarlet under the control of CamKIIa promoterDepositorInsertChRmine
UseAAVTagsmScarletExpressionMutationPromoterCaMKIIaAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDB-hPER2-mScarlet-I-CD4-bla
Plasmid#179449PurposeDonor vector to knock in mScarlet-I C-terminal to human PER2 geneDepositorInsertmScarletI
UseCRISPRTagsHis/FlagExpressionMutationPromoterNoneAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only