We narrowed to 1,942 results for: pb

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pCMV-PE2

Plasmid

#132775

Purpose

Prime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3b

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

PE2

Tags

SV40 NLS

Expression

Mammalian

Mutation

See manuscript

Promoter

CMV

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-PE1

Plasmid

#132774

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

PE1

Tags

SV40 NLS

Expression

Mammalian

Mutation

See manuscript

Promoter

CMV

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shRNA beta-catenin

Plasmid

#18803

Purpose

3rd gen lentiviral vector for knocking down beta-catenin gene expression

Depositor

Bob Weinberg

Article

Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

Insert

b-catenin shRNA (CTNNB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

July 22, 2008

Availability

Academic Institutions and Nonprofits only

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pRK6-HA-TAK1

Plasmid

#44160

Depositor

Xin Lin

Article

Blonska et al J Biol Chem. 2005 Dec 30;280(52):43056-6

Insert

Tak1 (Map3k7 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Oct. 9, 2013

Availability

Academic Institutions and Nonprofits only
hSynapsin-PE2-Cterminal-lenti

Plasmid

#135956

Purpose

Neuronal expression of C-terminal intein-split prime editor v2 (PE2). This plasmid is used by PE2, PE3, and PE3b

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

Prime editor v2 (PE2) C-terminal

Use

Lentiviral

Expression

Mammalian

Mutation

MMLV RT(D200N, T306K, W313F, T330P, L603W)

Promoter

hSynapsin

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pADSS-EGFP

Plasmid

#228197

Purpose

expressed human ADSS with an EGFP fluorescent protein tag

Depositor

Stephen Benkovic

Article

Zhao et al J Biol Chem. 2015 Mar 13;290(11):6705-13

Insert

ADSS (ADSS1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBABE puro SDF-1 alpha

Plasmid

#12270

Depositor

Bob Weinberg

Article

Orimo et al Cell. 2005 May 6. 121(3):335-48.

Insert

SDF-1 alpha (CXCL12 Human)

Use

Retroviral

Expression

Mammalian

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

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GP804

Plasmid

#164548

Purpose

Expresses Smo-FLAG K543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

All sixteen Lysines in cytoplasmic C-terminal tai…

Promoter

GgCryD1

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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px-458-sgRNA-IF1

Plasmid

#206923

Purpose

Plasmid expressing Cas9, GFP and guides to human IF1 to generate IF1-KO mammalian cell lines

Depositor

José Cuezva

Article

Romero-Carraminana et al Commun Biol. 2023 Aug 12;6(1):836. doi:

Insert

sgRNA targeting IF1 (ATPase inhibitory factor 1) (ATP5IF1 Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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T40A+Q54A

Plasmid

#217913

Purpose

A plasmid encoding the hydrogel-forming protein PXP with both the T40A and Q54A coil mutations

Depositor

Cole DeForest

Article

Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d

Insert

PXP T40A+Q54A

Tags

6x His (N and C term)

Expression

Bacterial

Mutation

Threonine at position 41 and 253 mutated to Alani…

Promoter

T5

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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Borealin-mTAG-RFPt-3XFLAG

Plasmid

#191986

Purpose

Mammalian expression of PTK Borealin

Depositor

Paul Maddox

Insert

Borealin

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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U6-BbsI-crRNA

Plasmid

#51026

Purpose

Generates crRNA for use in combination with tracrRNA

Depositor

Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

Insert

U6-BbsI-crRNA

Use

CRISPR

Expression

Insect

Promoter

U6-2

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pRG02

Plasmid

#44196

Depositor

Denis Dupuy

Insert

Neo

Expression

Worm

Promoter

rps-27

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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pHR_PGK 3XFlag_4D5-5_CAR-mCherry

Plasmid

#247508

Purpose

Expresses a CAR against HER2 and mCherry

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

CAR against Her2 and mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-P2A-GFP

Plasmid

#132776

Purpose

Prime editing in mammalian cells with co-translational GFP expression

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

PE2-P2A-GFP

Tags

SV40 NLS

Expression

Mammalian

Mutation

See manuscript

Promoter

CMV

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-PE6d

Plasmid

#207854

Purpose

Mammalian expression of PE6d prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6d

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

M-MLVRTDRNAseHrt(T128N, V223Y, D200C)

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_ anti-NRCAM 12949 (VL + VH) synNotch

Plasmid

#247525

Purpose

Expresses a synNotch receptor binding to NRCAM

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

Expresses a synNotch receptor binding to NRCAM

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-Gal4UAS-GFP-PGK-BFP

Plasmid

#247506

Purpose

GAL4/UAS expression of GFP and constitutive expression of BFP

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

GAL4/UAS expression of GFP and constitutive expression of BFP

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-MMLVgag-3xNES-ABE8e-NG

Plasmid

#181754

Purpose

MMLV-Gag fused to 3xNES-ABE8e-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

Depositor

David Liu

Article

Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

Insert

MMLVgag-3xNES-ABE8e-NG

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-MMLVgag-3xNES-Cas9

Plasmid

#181752

Purpose

MMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.

Depositor

David Liu

Article

Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

Insert

MMLV-Gag-3xNES-Cas9 (Spy)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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