162,717 results

1. TFORF2991

   Plasmid

   #144467

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ID2 (ID2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-Cas9-6xHis

   Plasmid

   #62374

   Purpose

   Expression of Cas9-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   Cas9_6xHis
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. LMAN2-bio-His

   Plasmid

   #51923

   Purpose

   Expresses full-length Vesicular integral-membrane protein VIP36 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   LMAN2 (LMAN2 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lentiviral nontargeting shRNA RFP i670

   Plasmid

   #155287

   Purpose

   Lentiviral expression of nontargeting shRNA, RFP i670 expression, based on Addgene 12247

   Depositor

   Martin Carroll

   Insert

   Nontargeting shRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. SPLICS Po-ER Long P2A

   Plasmid

   #164113

   Purpose

   Detect the long Peroxisomes-Endoplasmic reticulum contact

   Depositor

   Tito Calì, Marisa Brini

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split YFP Po-ER Long P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. eiCBE

   Plasmid

   #227148

   Purpose

   Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with two copies of UGI and hA3A* variant driven by CMV promoter

   Depositor

   Dali Li

   Article

   Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e

   Insert

   bpNLS-hA3A*-linker-nOgeuIscB-v3-nucleoplasmin NLS-2XUGI-bpNLS
   

   Use

   Tags

   Expression

   Mutation

   D96R/E84R/V159R/H339A

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223_DNMT3A_WT

   Plasmid

   #81765

   Purpose

   Gateway Donor vector containing DNMT3A , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, Jesse Boehm, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   DNMT3A (DNMT3A Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28 MBP_PrA/G-H6

   Plasmid

   #176077

   Purpose

   Express His6 tagged MBP_PrA/G in Escherichia coli

   Depositor

   Tom Rapoport

   Article

   Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

   Insert

   MBP PrA/G-H6
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9_T2A_iRFP670 sgBFP

   Plasmid

   #192478

   Purpose

   All-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Use

   Tags

   T2A iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP-AAV-syn-jGCaMP8f-WPRE

    Plasmid

    #162376

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Q315L

    Promoter

    Synapsin

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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