We narrowed to 2,123 results for: PAM

1. PL452-FGF8-LA-p2a-tdTomato-hGHpA-PGK-neoR-FGF8-RA

   Plasmid

   #191687

   Purpose

   For knocking p2a-tdTomato into FGF8 locus

   Depositor

   Yuejun Chen

   Article

   Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

   Insert

   FGF8 homology arms and p2a-tdTomato
   

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. PL452-PTPRO-LA-p2a-EGFP-hGHpA-PGK-neoR-PTPRO-RA

   Plasmid

   #191689

   Purpose

   For knocking p2a-EGFP into PTPRO locus

   Depositor

   Yuejun Chen

   Article

   Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

   Insert

   PTPRO homology arms and p2a-EGFP
   

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKM5

   Plasmid

   #154166

   Purpose

   Aedes aegypti Rel2 (long isoform) expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   Rel2 (long isoform)
   

   Tags

   FLAG

   Expression

   Insect

   Promoter

   IE1 promoter and hr5 enhancer from Autographa cal…

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKM28

   Plasmid

   #154165

   Purpose

   Aedes aegypti Rel1A expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   Rel1A
   

   Tags

   FLAG

   Expression

   Insect

   Promoter

   IE1 promoter and hr5 enhancer from Autographa cal…

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAA2

   Plasmid

   #154162

   Purpose

   Firefly luciferase under the control of 4 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Mutation

   16 wt NF-κB sites were replaced with Rel1A-specif…

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAA1

   Plasmid

   #154161

   Purpose

   Firefly luciferase under the control of 3 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Mutation

   12 wt NF-κB sites were replaced with Rel1A-specif…

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKM62

   Plasmid

   #154160

   Purpose

   Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel2-specific (VII) NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Mutation

   4 wt NF-κB sites were replaced with Rel2-specific…

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKM59

   Plasmid

   #154158

   Purpose

   Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, a TATA box and Kozak sequence of the Metchnikowin gene and additional NF-κB site.

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Promoter

   Enhancer for the Drosophila melanogaster Metchnik…

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKM52

   Plasmid

   #154155

   Purpose

   Firefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel2 NF-κB site

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Promoter

   Drosophila melanogaster minimal Hsp70 promoter wi…

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKM51

    Plasmid

    #154154

    Purpose

    Firefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel1A NF-κB site

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Promoter

    Drosophila melanogaster minimal Hsp70 promoter wi…

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmCherry-Synaptojanin-1-145

    Plasmid

    #223563

    Purpose

    Expresses the short isoform of SYNJ1 (neuronal) with mCherry tagged at the n-terminus

    Depositor

    Pietro De Camilli

    Article

    Mohd Rafiq et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

    Insert

    SYNJ1 (SYNJ1 Human)
    

    Tags

    His and mCherry

    Expression

    Mammalian

    Available Since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV_CAG_FLEX_PKIα-P2A-mRuby3_WPRE

    Plasmid

    #196677

    Purpose

    AAV plasmid for mammalian Cre-dependent expression of PKIα-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIα (protein kinase inhibitor α) inhibits the activity of protein kinase A (PKA).

    Depositor

    Haining Zhong

    Article

    Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

    Insert

    PKIα-P2A-mRuby3
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV_CAG_FLEX_PKIαmut-P2A-mRuby3_WPRE

    Plasmid

    #196678

    Purpose

    AAV plasmid for mammalian Cre-dependent expression of PKIαmut-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIαmut bears the R20G/R21G mutation that renders it non-functional.

    Depositor

    Haining Zhong

    Article

    Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

    Insert

    PKIαmut-P2A-mRuby3
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-FLAG-MTOR-R2505P

    Plasmid

    #69015

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-R2505P (MTOR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Arginine 2505 to Proline

    Available Since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-FLAG-MTOR-I2500F

    Plasmid

    #69014

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-I2500F (MTOR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Isoleucine 2500 to Phenylalanine

    Available Since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-FLAG-MTOR-E1799K

    Plasmid

    #69009

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-E1799K (MTOR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Glutamate 1799 to Lysine

    Available Since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid

    #139987

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-dLight1.2

    Plasmid

    #111068

    Purpose

    To generate Adeno-Associated viruses for expression of dLight1.2 under synapsin promoter

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Has Service

    AAV1 and AAV5

    Insert

    dLight1.2
    

    Use

    AAV

    Tags

    Flag tag

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-EF1a-double floxed-Gad67-WPRE-HGHpA

    Plasmid

    #184632

    Purpose

    Expresses Gad67, driven by the Ef1a promoter, in a Cre-dependent fashion

    Depositor

    Nicolas Tritsch

    Article

    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

    Insert

    Gad67 (Gad1 Mouse)
    

    Use

    AAV

    Promoter

    EF1a

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-pTH-iCre:EGFP-WPREpA

    Plasmid

    #213142

    Purpose

    Truncated rat TH promoter expressing iCre fused to EGFP

    Depositor

    Ulrik Gether

    Article

    Lycas et al Cell Rep. 2022 Sep 27;40(13):111431. doi

    Insert

    iCre
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    Truncated TH promoter from rat (Rattus norvegicus…

    Available Since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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