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pETM33_TNKS_TANK-peptide-bind

Plasmid

#178489

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

TNKS_TANK-peptide-bind (TNKS Human, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_MAD2L1_HORMA

Plasmid

#178480

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

MAD2L1_HORMA (MAD2L1 Human, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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PM2-FRB-CFP

Plasmid

#67518

Purpose

FRB conjugated plasma membrane targeting sequences 2 from Lyn11(GCIKSKGKDSA)

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

Insert

mTOR (MTOR Human)

Use

Tags

CFP, HA, and PM tethering sequence from human Lyn…

Expression

Mammalian

Mutation

FRB domain only (residues 2019–2114)

Promoter

CMV

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only
SARS_COV2_ORF3b_HA_BASU

Plasmid

#153992

Purpose

Express Orf3b gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

orf3b

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCRE-iRFP670

Plasmid

#82696

Purpose

Expresses CRE and iRFP670 under two separate PGK promoters.

Depositor

Alan Mullen

Article

Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

Insert

CRE, iRFP670

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-S2P-BA.4/5-AVI

Plasmid

#192112

Purpose

Expresses SARS-CoV-2 S2P protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Insert

SARS-CoV-2-S2P-BA.4/5 (S )

Use

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

T19I, L24S, P25-, P26-, A27-, H69-, V70-, G142D, …

Promoter

Available since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe puro RalA S31N

Plasmid

#19718

Depositor

Channing Der

Article

Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

Insert

V-ral simian leukemia viral oncogene homolog A (RALA Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

S31N mutant for dominant neg function. NOTE: lo…

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pLEX-FHH-MTOR HEAT

Plasmid

#120709

Purpose

Expresses Flag-HA-6xHIS-Mtor HEAT Domain fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

MTOR HEAT 1-9 (MTOR Human)

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Amino acids 1-409

Promoter

CMV

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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RET-SHC

Plasmid

#139617

Purpose

StrepIII-HA-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

RET (RET Human)

Use

Tags

StrepIII-HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shFXN_1

Plasmid

#102970

Purpose

Suppression of FXN

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shFXN_1 (FXN Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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MAC_C_FGFR2

Plasmid

#187751

Purpose

MAC-tagged gene expression of human FGFR2

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

FGFR2 (FGFR2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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ETV3L_pet28a

Plasmid

#131666

Purpose

Bacterial expression of ETS gene ETV3L

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV3L (ETV3L Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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ELK4_pet28a

Plasmid

#131648

Purpose

Bacterial expression of ETS gene ELK4

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELK4 (ELK4 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shFXN_2

Plasmid

#102971

Purpose

Suppression of FXN

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shFXN_2 (FXN Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pgRGFP

Plasmid

#82695

Purpose

Expresses gRNA of interest under U6 promoter (cloning by BpiI) and GFP under PGK promoter.

Depositor

Alan Mullen

Article

Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

PGK, U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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SARS_COV2_orf9c_HA_BASU

Plasmid

#153991

Purpose

Express Orf9c gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

orf9c

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCDFNKI-strepII-3C-LIC-strep

Plasmid

#108708

Purpose

Expression of a StrepII-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Use

Tags

StrepII-3C protease cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pETM33_KEAP1_KELCH

Plasmid

#178496

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

KEAP1_KELCH (KEAP1 Human, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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MEN2A-V5

Plasmid

#139629

Purpose

V5-His-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

MEN2A (RET Human)

Use

Tags

3xV5-6xHis

Expression

Mammalian

Mutation

C634W

Promoter

CMV

Available since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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MEN2B-V5

Plasmid

#139630

Purpose

V5-His-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

MEN2B (RET Human)

Use

Tags

3xV5-6xHis

Expression

Mammalian

Mutation

M918T

Promoter

CMV

Available since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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