We narrowed to 9,179 results for: sacs
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Plasmid#67568PurposeZebrafish expression of a photoactivatable fluorescent calcium reporterDepositorInsertspa-GCaMP6f
UseZebrafish transgenesis (see :a spinal opsin contr…TagsHIS-tagExpressionMutationK65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…PromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-α-COPI-WD40
Plasmid#180758PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 (residues 1-327)DepositorInsertα-COPI-WD40
UseTagsC-terminal strep tagExpressionMammalianMutationEncodes residues 1-327; mutations: Leu181Lys, Leu…PromoterCMVAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-chCARex
Plasmid#87233Purposebacterial expression of GST-CAR extracellular domainDepositorInsertCXADR (CXADR Chicken)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYTRW28K_0Ti1
Plasmid#177290Purposegenomic integration of genes (inserted in I-SceI-site) via rrn-interposon/SacB with TcRDepositorInsertPsacB-sacB
UseSynthetic Biology; Yeast expression, rrn genomic …TagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
ClhN-P1K-3VSV-Atg8(L55W T56A V61W R65A)-MET15
Plasmid#207054PurposeExpression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).DepositorInsertATG8
UseTags3xVSVExpressionYeastMutationL55W T56A V61W R65APromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
L_NLS-mutNLS V5-Twist
Plasmid#201381PurposeExpresses Twist mutant for nucleocytoplasmic transport studiesDepositorInsertTwist (Twist1 Mouse)
UseTags(NLS)SV40A4-V5ExpressionMammalianMutationK38R K73RPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
M_NLS-mutNLS V5-Twist
Plasmid#201379PurposeExpresses Twist mutant for nucleocytoplasmic transport studiesDepositorInsertTwist (Twist1 Mouse)
UseTags(NLS)SV40A5-V5ExpressionMammalianMutationK38R K73RPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
mutNLS V5-Twist
Plasmid#201380PurposeExpresses Twist mutant for nucleocytoplasmic transport studiesDepositorInsertTwist (Twist1 Mouse)
UseTagsV5ExpressionMammalianMutationK38R K73RPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
H_NLS-mutNLS V5-Twist
Plasmid#201378PurposeExpresses Twist mutant for nucleocytoplasmic transport studiesDepositorInsertTwist (Twist1 Mouse)
UseTags(NLS)SV40-V5ExpressionMammalianMutationK38R K73RPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
UL_NLS-mutNLS V5-Twist
Plasmid#201382PurposeExpresses Twist mutant for nucleocytoplasmic transport studiesDepositorInsertTwist (Twist1 Mouse)
UseTags(NLS)SV40A3-V5ExpressionMammalianMutationK38R K73RPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
mut GBP2 V5-Twist
Plasmid#201377PurposeExpresses Twist mutant for nucleocytoplasmic transport studiesDepositorInsertTwist (Twist1 Mouse)
UseTagsV5ExpressionMammalianMutationY107EPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Sandgrouse+R111Q–insD
Plasmid#191229PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationremoved aspartic acid at 121 and replaced arginin…PromoterPH and p10Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE059
Plasmid#176816PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert610
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
Clim Actin MCH Puro (CAMP)
Plasmid#104446PurposeExpresses mCherry under endogenous actin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope. Puromycin resistant.DepositorInsertmCherry
UseExpression in corallochytrium (single-celled euka…TagsExpressionMutationPromoteractinAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKdH_P-GAP-ScACS1*
Plasmid#126736Purposeyeast plasmid overexpressing ACS1 from Saccharomyces cerevisiaeDepositorInsertacs1*
UseTags6xHis,MycExpressionYeastMutationT3542C and A30S, P113S, and S629N (please see dep…PromoterGAPAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKdH_P-GAP-ScADH2+P-GAP*-ScACS1*
Plasmid#126738Purposeyeast plasmid overexpressing ADH2 and ACS1 from Saccharomyces cerevisiaeDepositorInsertadh2,acs1*
UseTags6xHis,MycExpressionYeastMutationT3542CPromoterGAPAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSG5-chCAR
Plasmid#87231PurposeMammalian expression of chicken CARDepositorInsertCXADR (CXADR Chicken)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
mClY-N1
Plasmid#90457PurposeA YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.DepositorInsertmClY-N1
UseTagsExpressionMammalianMutationEYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…PromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SaPE2
Plasmid#174817PurposeMammalian expression of SaCas9 prime editor 2DepositorInsertSaPE2
UseTagsSV40 bpNLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLIB-HIS-TEV-CKS1
Plasmid#177013PurposeGenerate baculovirus for insect cell expression of CKS1 with N-terminal Polyhistidine-TEVDepositorInsertCKS1 (CKS1B Human, Synthetic)
UseTagsPolyhistidine-TEVExpressionInsectMutationcodon-optimised for insect cell expressionPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYTRW26K_1Ti1
Plasmid#177292Purposegenomic integration of genes (inserted in I-SceI-site) via rrn-interposon/SacB with TcR and eYFPDepositorInsertPsacB-sacB
UseSynthetic Biology; Yeast expression, rrn genomic …TagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-EGFP-NLS-P2A-mCherry-PTS1
Plasmid#87803PurposeMammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using P2A. NLS and PTS1 can be replaced by proteins of interest.DepositorInsertsNLS
PTS1
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterCMV promoter, tetracycline operatorAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-EGFP-NLS-P2AT2A-mCherry-PTS1
Plasmid#87828PurposeHigh-efficient mammalian expression vector for co-expression of EGFP-tagged and mCherry-tagged proteins using P2AT2A. NLS and PTS1 can be replaced by proteins of interest.DepositorInsertsNLS
PTS1
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterCMV promoter, tetracycline operatorAvailable sinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSUMO-β′-COPI-WD40
Plasmid#180753PurposeBacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40 (residues 1-301).DepositorInsertβ′-COPI-WD40 (SEC27 Budding Yeast)
UseTagsN-terminal strep tag, His-tag, and SUMO tagExpressionBacterialMutationEncodes residues 1-301; deleted 302-889; mutated …PromoterT7Available sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-EGFP-NLS-T2A-mCherry-PTS1
Plasmid#87827PurposeMammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.DepositorInsertsNLS
PTS1
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterCMV promoter, tetracycline operatorAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
G31, Cdc20-AID tagging
Plasmid#130269PurposeTagging the Saccharomyces cerevisiae Cdc20 gene with a auxin-degron tag, via homologous recombinationDepositorInsertCdc20/AID(71-114)-tCYC1-NatMX (CDC20 Budding Yeast, Synthetic)
UseTagsAID(71-114)ExpressionYeastMutationPromoterNative promoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXs-M2O
Plasmid#188034PurposeFusion of human MYC Transactivation Domain (TAD) MYC Box I/II (MBI/II) with human OCT4 protein expressed in pMXs vectorDepositorInsertMYC TAD MBI/II region fused with OCT4 (POU5F1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKdH_P-GAP-ScADH2+P-GAP*-ScALD6+ScACS1*
Plasmid#126739Purposeyeast plasmid overexpressing ADH2,ALD6 and ACS1 from Saccharomyces cerevisiaeDepositorInsertadh2, acs1*, ald6
UseTags6xHis,MycExpressionYeastMutationT3542C and A30S, S629N (please see depositor comm…PromoterGAPAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYTRW27K_7Ti1
Plasmid#177294Purposegenomic integration of genes (inserted in I-SceI-site) via rrn-interposon/SacB with TcR and LacZDepositorInsertPsacB-sacB
UseSynthetic Biology; Yeast expression, rrn genomic …TagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
SSH-pSUMO-β′-COPI-WD40-Tyr33Ala
Plasmid#208170PurposeBacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40-Tyr33Ala mutant (residues 1-301)DepositorInsertN-terminal domain of yeast β′-COPI-WD40 subunit (1-301) with Tyr33Ala substitution
UseTagsHis-tag, SUMO-tag, and Strep-tagExpressionBacterialMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only