We narrowed to 19,627 results for: ATO

1. CMV-REX-GECO1

   Plasmid

   #61246

   Purpose

   Expresses REX-GECO1 in mammalian cells

   Depositor

   Robert Campbell

   Article

   Wu et al Nat Commun. 2014 Oct 31;5:5262. doi: 10.

   Insert

   REX-GECO1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Substitutions relative to R-GECO1: P60R, V61W, R6…

   Promoter

   CMV

   Available since

   March 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLKO.1 p18 shRNA

   Plasmid

   #26631

   Depositor

   David Sabatini

   Article

   Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

   Insert

   p18 shRNA (LAMTOR1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pDF0584 huDisCas7-11 S1006-GGGS-D1221 U6-NT guide

   Plasmid

   #186993

   Purpose

   AAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of non-targeting crRNA guide

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

   Insert

   cas7-11-s1006-gggs-d122, non-targeting crRNA guide
   

   Use

   AAV

   Tags

   Expression

   Mutation

   s1006-gggs-d1221 deletion

   Promoter

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1/Puro-CAG-ASAP2s

   Plasmid

   #101274

   Purpose

   Genetically encoded voltage indicator ASAP2s expressed under strong mammalian promoter (CAG)

   Depositor

   Francois St-Pierre

   Article

   Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

   Insert

   ASAP2s
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRE-UBC-hCD46-T2A-GFP

   Plasmid

   #205457

   Purpose

   lentiviral plasmid for expression of human CD46

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   hCD46 (CD46 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. hMLKL-Venus-Plasma

   Plasmid

   #106081

   Purpose

   expression in mammalian cells

   Depositor

   Douglas Green

   Article

   Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

   Insert

   hMLKL-Venus-Plasma (MLKL Human)
   

   Use

   Retroviral; Tet-on retrovirus

   Tags

   plasma membrane-targetting domain of H-Ras (aa 1-…

   Expression

   Mammalian

   Mutation

   Promoter

   dox-inducible

   Available since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. hMLKL NB(1-182)-2xFv-flag

   Plasmid

   #106075

   Purpose

   expression in mammalian cells

   Depositor

   Douglas Green

   Article

   Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

   Insert

   human MLKL NB(1-182)-2xFv-flag (MLKL Human)
   

   Use

   Retroviral; Tet-on retrovirus

   Tags

   flag

   Expression

   Mammalian

   Mutation

   amino acids 1-182

   Promoter

   Dox-inducible

   Available since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDF0430 huDisCas7-11-S1006-GGGS-D1221-U6-pro-Gluc

   Plasmid

   #186987

   Purpose

   AAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of Gluc crRNA guide

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

   Insert

   cas7-11-s1006-gggs-d122, Gluc crRNA guide
   

   Use

   AAV

   Tags

   Expression

   Mutation

   s1006-gggs-d1221 deletion

   Promoter

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. hMLKL NB(1-140)-2xFv-flag

   Plasmid

   #106074

   Purpose

   expression in mammalian cells

   Depositor

   Douglas Green

   Article

   Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

   Insert

   human MLKL NB(1-140)-2xFv-flag (MLKL Human)
   

   Use

   Retroviral; Tet-on retrovirus

   Tags

   flag

   Expression

   Mammalian

   Mutation

   amino acids 1-140

   Promoter

   Dox-inducible

   Available since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR223-TAF5-FL

    Plasmid

    #209047

    Purpose

    Gateway-compatible entry vector, with insert of the full length TAF5 gene

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    TAF5 full length
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPRE

    Plasmid

    #89587

    Purpose

    For nucleus-specific turboRFP labeling in single cells, pK263 should be used with pK031.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    nuclear localization signal (nls) turboRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCK301

    Plasmid

    #87767

    Purpose

    E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest to express using L-rhamnose, three terminators, ampR, pBR322 origin, lacI

    Depositor

    John Heap

    Article

    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1

    Insert

    PrhaBAD-sfGFP
    

    Use

    Synthetic Biology

    Tags

    6xHis Tag

    Expression

    Bacterial

    Mutation

    Promoter

    PrhaBAD rhamnose-inducible promoter from E. coli

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCFJ1955

    Plasmid

    #84830

    Purpose

    Minimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 selection. tdTomato was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic introns

    Depositor

    Andrew Fire

    Article

    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

    Insert

    C. elegans codon optimized tdTomato
    

    Use

    Tags

    Expression

    Bacterial and Worm

    Mutation

    Promoter

    Peft-3

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. hMLKL NB(1-140)-2xFv-Venus

    Plasmid

    #106076

    Purpose

    expression in mammalian cells

    Depositor

    Douglas Green

    Article

    Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

    Insert

    human MLKL NB(1-140)-2xFv-Venus (MLKL Human)
    

    Use

    Retroviral; Tet-on retrovirus

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    amino acids 1-140

    Promoter

    Dox-inducible

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ER-CanlonicSF

    Plasmid

    #178343

    Purpose

    To explore endoplasmic reticulum lactate dynamics

    Depositor

    Alejandro San Martín

    Article

    Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

    Insert

    ER-CanlonicSF
    

    Use

    Tags

    ER Retention Signal, ER Signal Exon 1, and ER Sig…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLCHKOv3

    Plasmid

    #209025

    Purpose

    Lentiviral backbone for expressing U6 driven hybrid guide (hg)RNAs with BveI cloning sites and puromycin selection marker

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-hSyn-ASAP2s

    Plasmid

    #101276

    Purpose

    Genetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for neuron-specific expression using the promoter hSyn1

    Depositor

    Francois St-Pierre

    Article

    Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

    Insert

    ASAP2s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn (human synapsin)

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti.tie1.H2B.eYFP.W

    Plasmid

    #51293

    Purpose

    Lentivector that encodes H2B-eYFP under the transcriptional control of the mouse TIE1 promoter

    Depositor

    Rusty Lansford

    Article

    Sato et al PLoS One. 2010 Sep 14;5(9):e12674. doi:

    Insert

    H2B-eYFP (H2BC21 Human)
    

    Use

    Lentiviral

    Tags

    H2B-eYFP

    Expression

    Mutation

    Promoter

    mTie1

    Available since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PB-CAG-DDdCas9VPP300-T2A-GFP-IRES-Neo

    Plasmid

    #102887

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO.1 MP1 shRNA

    Plasmid

    #26632

    Depositor

    David Sabatini

    Article

    Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

    Insert

    MP1 shRNA (LAMTOR3 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

21. PB-CAG-DDdCas9VPPH-T2A-GFP-IRES-Neo

    Plasmid

    #102888

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV_hSyn1_AchLightR

    Plasmid

    #187463

    Purpose

    Expresses red acetylcholine indicator AchLightR in neuronal cells

    Depositor

    Tommaso Patriarchi

    Article

    Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

    Insert

    AchLightR
    

    Use

    AAV

    Tags

    Igκ leader

    Expression

    Mutation

    Promoter

    human Synapsin-1

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDisplay-GACh1.0

    Plasmid

    #106072

    Purpose

    express the genetically-encoded fluorescent ACh indicator GACh1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

    Insert

    GPCR activation based ACh sensor (GACh) 1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. sgRNA1_ASCL1

    Plasmid

    #64129

    Purpose

    Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1_ASCL1 (ASCL1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSLIK-Hygro-Myc-hAgo2

    Plasmid

    #60234

    Purpose

    Express HA-tagged wild-type Ago2 in mammalian cells in a Dox-inducible manner

    Depositor

    Zissimos Mourelatos

    Article

    Liu et al Mol Cell. 2012 May 25;46(4):507-17. doi:

    Insert

    Argonaute2 (AGO2 Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

26. pLKO.1P POLE1_1

    Plasmid

    #160762

    Purpose

    Suppress POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLE1_1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDF0582 huDisCas7-11 R1045-GGGS-R1122 U6-NT guide

    Plasmid

    #186991

    Purpose

    AAV transgene plasmid for Cas7-11-R1045-GGGS-R1122, with U6 promoter-driven expression of non-targeting crRNA guide

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

    Insert

    cas7-11-r1045-gggs-r1122, non-targeting crRNA guide
    

    Use

    AAV

    Tags

    Expression

    Mutation

    r1045-gggs-r1122 deletion

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEGFP-C1-IFT27

    Plasmid

    #218722

    Purpose

    Expresses N-terminally EGFP-tagged IFT27 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

    Insert

    IFT27 (IFT27 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. sgRNA2_ASCL1

    Plasmid

    #64130

    Purpose

    Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1_ASCL2 (ASCL1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pWZL-hygro-FAKY925F

    Plasmid

    #216547

    Purpose

    This retroviral plasmid expresses a mutated cDNA (dominant negative: Y925F ) of human PTK2

    Depositor

    Georgia Konstantinidou

    Article

    Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

    Insert

    PTK2 (Y925F) (PTK2 Human)
    

    Use

    Retroviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    FAK dominant negative mutation Y925F

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. hMLKL-Venus-Mito

    Plasmid

    #106080

    Purpose

    expression in mammalian cells

    Depositor

    Douglas Green

    Article

    Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

    Insert

    hMLKL-Venus-Mito (MLKL Human)
    

    Use

    Retroviral; Tet-on retrovirus

    Tags

    mitochondrial-targetting domain of Bcl-xl

    Expression

    Mammalian

    Mutation

    Promoter

    Dox-inducible

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pK098.CAG-loxP-stop-loxP-nlstagRFP-ires-tTA-WPRE (Supernova)

    Plasmid

    #85179

    Purpose

    For nucleus-specific RFP labeling in single cells, pK098 should be used with pK031.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    nuclear localization signal (nls) tagRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. sgRNA4_ASCL1

    Plasmid

    #64132

    Purpose

    Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1_ASCL4 (ASCL1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLKO.1P POLD1_2

    Plasmid

    #160792

    Purpose

    Suppress POLD1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLD1_2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. sgRNA3_ASCL1

    Plasmid

    #64131

    Purpose

    Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1_ASCL3 (ASCL1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLKO.1P POLE1_2

    Plasmid

    #160764

    Purpose

    Suppress POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLE1_2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. inhibin-DsRed

    Plasmid

    #156184

    Purpose

    Expression plasmid for production of transgenic rainbow trout strain carrying the DsRed gene under control of the inhibin α promoter.

    Depositor

    Goro Yoshizaki

    Article

    Iwasaki-Takahashi et al Commun Biol. 2020 Jun 15;3(1):308. doi:

    Inserts

    rainbow trout inhibin α promoter
    rainbow trout β actin 3'UTR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    inhibin

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PB-tight-DDdCas9VPH-T2A-GFP-IRES-Neo

    Plasmid

    #102890

    Purpose

    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE-tight

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PB-tight-DDdCas9VPPH-T2A-GFP-IRES-Neo

    Plasmid

    #102892

    Purpose

    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE-tight

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLKO.1P DDX11_2

    Plasmid

    #160778

    Purpose

    Suppress DDX11

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shDDX11_2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pLKO.1P DDX11_1

    Plasmid

    #160777

    Purpose

    Suppress DDX11

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shDDX11_1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. sgRNA1_IL1RN

    Plasmid

    #64140

    Purpose

    Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    IL1RN (IL1RN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pMXS-IRES-Blast SHMT2res

    Plasmid

    #106301

    Purpose

    Expresses SHMT2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    serine hydroxymethyltransferase 2 (SHMT2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Silent mutations destroy sgRNA targeting sites

    Promoter

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. PB-tight-DDdCas9VPP300-T2A-GFP-IRES-Neo

    Plasmid

    #102891

    Purpose

    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE-tight

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pLKO.1P POLE2

    Plasmid

    #160766

    Purpose

    Suppress POLE2

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLE2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. sgRNA3_IL1RN

    Plasmid

    #64151

    Purpose

    Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA3_IL1RN (IL1RN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pLKO.1P POLD1_1

    Plasmid

    #160791

    Purpose

    Suppress POLD1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLD1_1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pLKO.1P REV3L_2

    Plasmid

    #160798

    Purpose

    Suppress REV3L

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shREV3L_2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pLKO.1P REV3L_1

    Plasmid

    #160797

    Purpose

    Suppress REV3L

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shREV3L_1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pLKO.1P POLA1_2

    Plasmid

    #160790

    Purpose

    Suppress POLA1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLA1_2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge