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We narrowed to 24,945 results for: Spr

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Showing: 3301 - 3350 of 24945 results

  4. RFN_EGFP_47

    Plasmid
    #60071
    Purpose
    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease
    Depositor
    Keith Joung
    Article
    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103
    Insert
    pair of multiplex gRNAs targeting EGFP
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Oct. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. p305N-30

    Plasmid
    #246313
    Purpose
    Evaluation of PtU6.2c3 promoter (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line
    Depositor
    Chung-Jui Tsai
    Article
    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294
    Insert
    PtU6.2c3 promoter
    Use
    CRISPR
    Expression
    Plant
    Mutation
    deletion: -T (-29 from TSS) within TATA
    Promoter
    PtU6.2c3 promoter
    Available Since
    Oct. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    246313-plasmid-map-sequence-id-491198

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  18. p305N-31 (nonfunctional)

    Plasmid
    #246314
    Purpose
    Evaluation of PtU6.2c4 promoter (nonfunctional) (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line
    Depositor
    Chung-Jui Tsai
    Article
    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294
    Insert
    PtU6.2c4 promoter (nonfunctional)
    Use
    CRISPR
    Expression
    Plant
    Mutation
    deletions: -A (-41 from TSS), -T (-30), -T (-29);…
    Promoter
    PtU6.2c4 promoter (nonfunctional)
    Available Since
    Oct. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    246314-plasmid-map-sequence-id-491197

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  19. p305N-9 (nonfunctional)

    Plasmid
    #246292
    Purpose
    Evaluation of AtU6.29c13 promoter (nonfunctional) (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line
    Depositor
    Chung-Jui Tsai
    Article
    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294
    Insert
    AtU6.29c13 promoter (nonfunctional)
    Use
    CRISPR
    Expression
    Plant
    Mutation
    deletions: -A (-58 from TSS), -T (-57) within USE
    Promoter
    AtU6.29c13 promoter (nonfunctional)
    Available Since
    Oct. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    246292-plasmid-map-sequence-id-491207

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  20. p305N-17 (nonfunctional)

    Plasmid
    #246300
    Purpose
    Evaluation of HbU6.2m3 promoter (nonfunctional) (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line
    Depositor
    Chung-Jui Tsai
    Article
    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294
    Insert
    HbU6.2m3 promoter (nonfunctional)
    Use
    CRISPR
    Expression
    Plant
    Mutation
    C-to-T (-29 from TSS), G-to-A (-24)
    Promoter
    HbU6.2m3 promoter (nonfunctional)
    Available Since
    Oct. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    246300-plasmid-map-sequence-id-491174

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  21. p305N-4 (nonfunctional)

    Plasmid
    #246287
    Purpose
    Evaluation of AtU6.1c1 promoter (nonfunctional) (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line
    Depositor
    Chung-Jui Tsai
    Article
    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294
    Insert
    AtU6.1c1 promoter (nonfunctional)
    Use
    CRISPR
    Expression
    Plant
    Mutation
    G-to-C at -15 from TSS
    Promoter
    AtU6.1c1 promoter (nonfunctional)
    Available Since
    Oct. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    246287-plasmid-map-sequence-id-491143

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  22. p305N-26 (nonfunctional)

    Plasmid
    #246309
    Purpose
    Evaluation of MtU6.6m7 promoter (nonfunctional) (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line
    Depositor
    Chung-Jui Tsai
    Article
    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294
    Insert
    MtU6.6m7 promoter (nonfunctional)
    Use
    CRISPR
    Expression
    Plant
    Mutation
    C-to-A (-63 from TSS), T-to-G (-57)
    Promoter
    MtU6.6m7 promoter (nonfunctional)
    Available Since
    Oct. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    246309-plasmid-map-sequence-id-491101

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  32. pMKR07

    Plasmid
    #240097
    Purpose
    A CRISPR‑Cas9 system for knock‑out and knock‑in of high molecular weight DNA enables module‑swapping of the pikromycin synthase in its native host
    Depositor
    Adrian Keatinge-Clay
    Article
    Wang et al Res Sq [Preprint]. 2025 Mar 27:rs.3.rs-6
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    ermE* promoter
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    240097-plasmid-map-sequence-id-478442

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  33. pYPQAT16

    Plasmid
    #223388
    Purpose
    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for monocot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-hA3A-Y130F-SpCas9-D10A-UGI-OsU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    223388-plasmid-map-sequence-id-474023

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  34. pYPQAT15

    Plasmid
    #223387
    Purpose
    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by 2x35s and the sgRNA was driven by AtU3 promoter.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-hA3A-Y130F-SpCas9-D10A-UGI-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223387-plasmid-map-sequence-id-452296

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  35. pYPQAT26

    Plasmid
    #223398
    Purpose
    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and the sgRNA was driven by AtU3; BASTA for plants select.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223398-plasmid-map-sequence-id-452310

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  36. pYPQAT28

    Plasmid
    #223400
    Purpose
    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and sgRNA was driven by OsU3; Hygromycin for plant select.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-OsU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223400-plasmid-map-sequence-id-452334

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  37. pYPQAT29

    Plasmid
    #223401
    Purpose
    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and the sgRNA was driven by OsU3; BASTA for plant select.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-OsU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223401-plasmid-map-sequence-id-452216

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  38. pYPQAT30

    Plasmid
    #223402
    Purpose
    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 and the sgRNA was driven by separate ZmUbi1; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-ZmUbi-gRNA scaffold 2.0-tRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223402-plasmid-map-sequence-id-454247

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  39. pYPQAT14

    Plasmid
    #223386
    Purpose
    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and sgRNA was by AtU3; BASTA for plant selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-hA3A-Y130F-SpCas9-D10A-UGI-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223386-plasmid-map-sequence-id-454178

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  44. pRhaCAST

    Plasmid
    #211791
    Purpose
    pZLrhaB2plus with Sh-cas12K-tnsBC-tniQ cloned into, which drives by the rhamnose inducible promoter
    Depositor
    Silvia Cardona
    Article
    Yap et al Appl Environ Microbiol. 2024 Jul 24;90(7
    Insert
    ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    Rhamnose-inducible
    Available Since
    June 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    211791-plasmid-map-sequence-id-418380

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Showing: 3301 - 3350 of 24945 results