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pBABE-puro-GFP-progerin

Plasmid

#17663

Depositor

Tom Misteli

Article

Scaffidi et al Nat Cell Biol. 2008 Mar 2. ():.

Insert

GFP-D50 lamin A (LMNA Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Delta 50

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-GFP-wt-lamin A

Plasmid

#17662

Depositor

Tom Misteli

Article

Scaffidi et al Nat Cell Biol. 2008 Mar 2. ():.

Insert

GFP-lamin A (LMNA Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Zeo_3xSH2(GRB2)-CTEV-2xHA

Plasmid

#214614

Purpose

adapter for RTK split TEV recruitment assays

Depositor

Michael Wehr

Article

Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

Insert

3xSH2 domains of GRB2-CTEV (GRB2 Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pQC hGAB.wt V5-His IRES G418

Plasmid

#110333

Purpose

Mammalian retroviral expression of glutaminase 2 isoform GAB (wild type) with V5 and 6xHis tags

Depositor

Sandra Martha Gomes Dias

Article

Dias et al Oncogene. 2020 Jan;39(3):690-702. doi: 1

Insert

GLS2 Glutaminase 2 (GLS2 Human)

Use

Retroviral

Tags

6xHis and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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BRI1 BRI1_pECIA2

Plasmid

#115086

Purpose

Bait vector BRI1 BRI1_pECIA2 should be used with prey vector BRI1 BRI1_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT4G39400 (BRI1 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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RPK2 X042_pECIA2

Plasmid

#115135

Purpose

Bait vector RPK2 X042_pECIA2 should be used with prey vector RPK2 X042_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT3G02130 (RPK2 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_7XCoordinatorMutant

Plasmid

#173994

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with all 7 Coordinator motifs mutated

Use

Luciferase

Tags

Expression

Mutation

7X Coordinator motif mutation #1-2-3-4-5-6-7

Promoter

SV40 promoter

Available since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9.scr_1

Plasmid

#190703

Purpose

Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Scrambled sgRNA 1

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Drosophila U6

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9.scr_3

Plasmid

#190705

Purpose

Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Scrambled sgRNA 3

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Drosophila U6

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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IL2-AH-2A-Cherry-RhoAQ63L in pmCherryN1

Plasmid

#187287

Purpose

Express pmCherry-IL2-AH-2A-RhoA Q63L

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

interleukin-2 receptor alpha-subunit, anillin, RhoA (IL2, ANLN, RHOA Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

AH-2A-RhoA Q63L

Promoter

CMV

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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IL2-AHDC2-2A-CherryRhoAQ63L in pmCherryN1

Plasmid

#187292

Purpose

Express pmCherry-IL2-AH-deltaC2-2A-RhoA Q63L

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

interleukin-2 receptor alpha-subunit, anillin, RhoA (IL2, ANLN, RHOA Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

AH-deltaC2, RhoA Q63L

Promoter

CMV

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-MND-OsTIR1(F74G)-P2A-EGFP-AID2-EF1a-RFP

Plasmid

#232800

Purpose

Helper plasmid to trigger auxin-inducible-degradation of targeted proteins.

Depositor

Chunliang Li

Article

Hyle et al Genome Biol. 2023 Jan 26;24(1):14. doi:

Insert

OsTIR1 F74G EGFP-AID

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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3ME6/mSprouty2

Plasmid

#22097

Depositor

Gail Martin

Article

Minowada et al Development. 1999 Oct . 126(20):4465-75.

Insert

Sprouty 2 (Spry2 Mouse)

Use

Bacterial

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
3CB1/cSprouty2

Plasmid

#22096

Depositor

Gail Martin

Article

Minowada et al Development. 1999 Oct . 126(20):4465-75.

Insert

Sprouty 2 (SPRY2 Chicken)

Use

Bacterial

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
Gatewayp221oxCerulean-oxVenus

Plasmid

#68047

Purpose

oxCerulean-oxVenus in Gateway [1-2] plasmid p221

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

p221oxCerulean-oxVenus

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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PP_070_pAAV_CAG_LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2

Plasmid

#203228

Purpose

Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

Depositor

Adam Cohen

Article

Park et al bioRxiv. 2023 Jun 2:2023.06.02.543490. d

Insert

Membrane-localized optopatch for neuronal dendrites

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pW228-lenti-sg1-mmSerpinh1-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189944

Purpose

Lentiviral vector to co-express a mouse Serpinh1 spsgRNA (sg1-Serpinh1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Serpinh1 spsgRNA #1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pW230-lenti-sg3-mmSerpinh1-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189946

Purpose

Lentiviral vector to co-express a mouse Serpinh1 spsgRNA (sg3-Serpinh1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Serpinh1 spsgRNA #3

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pW317-lenti-sg1-mmItga9-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189947

Purpose

Lentiviral vector to co-express a mouse Itga9 spsgRNA (sg1-Itga9) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Itga9 spsgRNA #1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pW319-lenti-sg3-mmItga9-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189949

Purpose

Lentiviral vector to co-express a mouse Itga9 spsgRNA (sg3-Itga9) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Itga9 spsgRNA #3

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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