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EGFP-SopB

Plasmid

#183657

Purpose

N-terminally tagged Salmonella Typhimurium SopB for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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SGyA

Plasmid

#160292

Purpose

Drives expression of Cas9 and a TdTomato fluorescent marker

Depositor

Omar Akbari

Article

Gamez et al Nat Commun. 2021 Dec 10;12(1):7202. doi:

Inserts

Vas promoter
hSpCas9
tdTomato
CTCF insulator
Gypsy insulator
Left homology arm Y chromosome
Right homology arm Y chromosome

Tags

T2A GFP

Expression

Insect

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a(+) NRP1 b1b2 (273-586)

Plasmid

#162733

Purpose

Bacterial expression the b1b2 tandem domains from the rat Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.

Depositor

Brett Collins

Article

Daly et al Science. 2020 Oct 20. pii: science.abd30

Insert

Human NRP1 b1b2 domains (residues 273-586)

Tags

6His

Expression

Bacterial

Mutation

Codon optimised

Available Since

March 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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EGFP-IpgD(C439S)

Plasmid

#183667

Purpose

N-terminally tagged Shigella flexneri IpgD (catalytically inactive) for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

IpgD

Tags

EGFP

Expression

Mammalian

Mutation

C439S

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-Nkx2-1*/Neo

Plasmid

#31272

Depositor

Tyler Jacks, Monte Winslow

Article

Winslow et al Nature. 2011 May 5. 473(7345):101-4.

Insert

Nkx2-1* (Nkx2-1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

A shNkx2-1 insensitive Nkx2-1 cDNA was created by…

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HtrA2-FLAG S400D

Plasmid

#15940

Depositor

L. Miguel Martins

Article

Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

Insert

HtrA2 (HTRA2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S400D

Available Since

Nov. 5, 2007

Availability

Academic Institutions and Nonprofits only
pET28a-his-Gluc-iLID

Plasmid

#172096

Purpose

Expresses a fusion protein of Gluc and iLID, which dimerizes with a sspB-tagged protein when the bioluminescent reaction is initiated, in bacteria

Depositor

Avi Schroeder

Article

Adir et al Nat Commun. 2022 Apr 28;13(1):2328. doi:

Insert

Gaussia Luciferase - iLID

Expression

Bacterial

Mutation

Gluc Mutations - M43L, M110L

Promoter

T7

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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SopB-EGFP

Plasmid

#183655

Purpose

C-terminally tagged Salmonella Typhimurium SopB for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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MAP4K3-GFP K45E

Plasmid

#21734

Depositor

L. Miguel Martins

Article

Lam et al Proc Natl Acad Sci U S A. 2009 Jul 21;10

Insert

MAP4K3 (MAP4K3 Human)

Tags

GFP

Expression

Mammalian

Mutation

Mutation of the ATP-binding site (K45E). This ver…

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only
pESC-LEU-SopB

Plasmid

#183670

Purpose

Inducible Salmonella Typhimurium SopB for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Expression

Yeast

Promoter

GAL1

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pESC-Leu-SopB(C460S)

Plasmid

#183671

Purpose

Inducible Salmonella Typhimurium SopB (catalytically inactive) for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Expression

Yeast

Mutation

C460S

Promoter

GAL1

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-Gluc-EL222-his

Plasmid

#172097

Purpose

Expresses the fusion protein of Gluc and the light-activated transcription factor EL222 in bacteria

Depositor

Avi Schroeder

Article

Adir et al Nat Commun. 2022 Apr 28;13(1):2328. doi:

Insert

Gaussia Luciferase - EL222

Expression

Bacterial

Mutation

Gluc mutations - M43L, M110L

Promoter

T7

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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His6-SMT3-SopB

Plasmid

#183676

Purpose

Recombinant Salmonella Typhimurium SopB

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Budding Yeast, Salmonella enterica serovar Typhimurium)

Tags

His6-S. cerevisiae SMT3(2-101)

Expression

Bacterial

Promoter

T7

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HtrA2-FLAG S142D

Plasmid

#15939

Depositor

L. Miguel Martins

Article

Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

Insert

HtrA2 (HTRA2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S142D

Available Since

Nov. 5, 2007

Availability

Academic Institutions and Nonprofits only
Rb-379-928Δ703-737-pGex2T

Plasmid

#21933

Depositor

William Kaelin

Article

Kaelin et al Cell. 1991 Feb 8. 64(3):521-32.

Insert

Rb (RB1 Human)

Tags

GST

Expression

Bacterial

Mutation

deletion mutant ORF contains aa 379-928D703-737 o…

Available Since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

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MAP4K3-GFP d344-873

Plasmid

#21732

Depositor

L. Miguel Martins

Article

Lam et al Proc Natl Acad Sci U S A. 2009 Jul 21;10

Insert

MAP4K3 (MAP4K3 Human)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of the carboxy-terminus, amino-acids 344…

Available Since

June 3, 2010

Availability

Academic Institutions and Nonprofits only
MAP4K3-GFP d1-343

Plasmid

#21733

Depositor

L. Miguel Martins

Article

Lam et al Proc Natl Acad Sci U S A. 2009 Jul 21;10

Insert

MAP4K3 (MAP4K3 Human)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of the amino-terminus, amino-acids 1 to …

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
Rb-379-928Δ738-775-pGex2T

Plasmid

#21783

Depositor

William Kaelin

Article

Kaelin et al Cell. 1991 Feb 8. 64(3):521-32.

Insert

Rb (RB1 Human)

Tags

GST

Expression

Bacterial

Mutation

deletion mutant ORF contains aa 379-928D737-774 o…

Available Since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

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EGFP-SopB(K525A)

Plasmid

#183659

Purpose

N-terminally tagged Salmonella Typhimurium SopB (K525A) for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Tags

EGFP

Expression

Mammalian

Mutation

K525A

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-SopB(K528A)

Plasmid

#183660

Purpose

N-terminally tagged Salmonella Typhimurium SopB (K528A) for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

sopB (sopB Salmonella enterica serovar Typhimurium)

Tags

EGFP

Expression

Mammalian

Mutation

K528A

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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