We narrowed to 20,110 results for: IRE;
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Plasmid#204547PurposeExpresses 3xFLAG-tagged NBR1 deltaUBA in mammalian cellsDepositorAvailable SinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only
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pPB-CAG-Dnmt3a2-Ires2-mCherry-SV40
Plasmid#186969PurposePiggyBac vector encoding mouse Dnmt3a2 with Ires2-mCherry fluorescent marker for expression in mammalian cells.DepositorInsertDnmt3a2 (Dnmt3a Synthetic, Mouse)
UsePiggybacTagsFlagExpressionMammalianMutationIsoform 2 (residues 220ā908)PromoterCAGAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-tight-DDdCas9VPP300-T2A-GFP-IRES-Neo
Plasmid#102891PurposePiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-P300-T2A-GFP-IRES-Neo
UseCRISPRExpressionMammalianMutationD10A, H840APromoterTRE-tightAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-tmeGFP-IRES-nls-mRuby2
Plasmid#63060PurposeExpresses truncated monomeric eGFP and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backboneDepositorInsertnls-mRuby2
UseAAV and Cre/LoxExpressionMammalianPromoterCAGAvailable SinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Cterm_3xFL_BRD9_BET-PGK-Puro-IRES-GFP
Plasmid#75123PurposeRetroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4 with a C terminal 3x FLAG-tagDepositorInsertBRD9 (BRD9 Human)
UseRetroviralTags3x FLAG-tagMutationbromodomain replaced with the first bromodomain oā¦Available SinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-MCS-dOB-hPOT1_IRES-blast
Plasmid#166413Purposeexpress hPOT1 ĪOB in mammalian cellsDepositorInsertProtection Of Telomeres 1 (POT1 Human)
UseLentiviralTagsmycExpressionMammalianMutationĪOB deleted 125 a.aPromoteref1-aAvailable SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLe6Ī -hLP-mcs/(EGFP-IresPuro-hInt)
Plasmid#64314Purposechondrocyte-specific COL11A2 promoter/enhancer lentiviral reporter vector to select iChon cellsDepositorUseLentiviralTagsEGFP and IRES-PUROExpressionMammalianMutationN/AAvailable SinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-PolĆ(D256A)-IRES-puro
Plasmid#128677Purposemammalian expression of Flag-PolĆ(D256A) protein with puromycin selectionDepositorAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRD9_N216A-PGK-Puro-IRES-GFP
Plasmid#75116PurposeRetroviral expression plasmid encoding a human BRD9 mutant carrying the N216A single amino acid mutationDepositorAvailable SinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT2K-CAGGS-mouse Gfi1-IRES-GFP
Plasmid#91893PurposeMammalian expression of mouse Gfi1DepositorAvailable SinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST flag-CAD hCPS1 AD
Plasmid#188130PurposeExpresses C-terminal flag-tagged human CAD hCPS1 AD in mammalian cellsDepositorInsertCAD (CAD Human)
UseRetroviralTagsFlag tagExpressionMammalianMutationTCC -> AGT silent mutations at nt439-441 elimiā¦Available SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-C-Myc-DDK-IRES-Puro HNRNPR
Plasmid#200730PurposeLentiviral expression of Flag-Tagged human HNRNPRDepositorInsertHNRNPR (HNRNPR Human)
UseLentiviralTagsMyc-DDK from backbone plasmidExpressionMammalianPromoterCMVAvailable SinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRD9_BET-PGK-Puro-IRES-GFP
Plasmid#75120PurposeRetroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4DepositorInsertBRD9 (BRD9 Human)
UseRetroviralMutationBromodomain replaced with the first bromodomain oā¦Available SinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
Direct-seq_lentiGuide-Puro-tRNA-Tail-8A8G
Plasmid#157984PurposeExpresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.DepositorInsertS. pyogenes sgRNA cassette (tRNA-Tail-8A8G)
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPB CAG Gfi1:FLAG-IRES-EGFP
Plasmid#153945PurposepiggyBac transposon vector with CAG promoter expressing FLAG-tagged Gfi1 and EGFPDepositorAvailable SinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-3xFLAG-MyD88-GyrB
Plasmid#131350PurposeExpresses MyD88 with an N-termial 3xFLAG tag and a C-terminal GyrB domain from the E. coli DNA Gyrase BDepositorAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST flag-CAD E1368A
Plasmid#188141PurposeExpresses C-terminal flag-tagged human CAD E1368A in mammalian cellsDepositorInsertCAD (CAD Human)
UseRetroviralTagsFlag tagExpressionMammalianMutationTCC -> AGT silent mutations at nt439-441 elimiā¦Available SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLK89 - Edit-directing plasmid cloning insert
Plasmid#98846PurposeContains the insert used for step 2 of edit-directed plasmid cloningDepositorInsertKanR
UseCRISPRExpressionYeastMutationChanged sequence to remove a MluI cut siteAvailable SinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST flag-CAD S1396A
Plasmid#188126PurposeExpresses C-terminal flag-tagged human CAD S1396A in mammalian cellsDepositorInsertCAD (CAD Human)
UseRetroviralTagsFlag tagExpressionMammalianMutationTCC -> AGT silent mutations at nt439-441 elimiā¦Available SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB CAG Cdk6 R31C-IRES-EGFP
Plasmid#153946PurposepiggyBac transposon vector with CAG promoter expressing Cdk6 (R31C mutant) and EGFPDepositorAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPB CAG SMARCA4 T910M-IRES-EGFP
Plasmid#153949PurposepiggyBac transposon vector with CAG promoter expressing T910M mutant SMARCA4 (FLAG-tagged) and EGFPDepositorAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
M10.2-IRES-tauVenus-ACNF Targeting Vector
Plasmid#15858DepositorInsertM10.2
ExpressionBacterialAvailable SinceMay 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-MyD88 (S34Y)-CpLxIS
Plasmid#131349PurposeExpresses MyD88 S34Y with 2 copies of pLxIS motif from STING fused to its C-terminusDepositorAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV-miR30_shBrd9_1116-PGK-NeoR-IRES-mCherry
Plasmid#75133PurposeRetroviral expression plasmid encoding shBrd9_1116 (targeting murine Brd9)DepositorInsertshBrd9_1116
UseRetroviralAvailable SinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAG-FNLS-T2A-EGFP-ires-puro
Plasmid#121169PurposeCAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.DepositorInsertFNLS-T2A-EGFP-ires-puro
UseCRISPRTagsT2A-EGFP-ires-puroExpressionMammalianAvailable SinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWPT-ACC/ACC-mEGFP-IRES-mCherry
Plasmid#49229PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianPromoterEF1-shortAvailable SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
cTRE-LSL-GFP-IRES-Cas9-sgCR8
Plasmid#135668PurposeIntroduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus control-targeting sgRNA into (ES) cells by recombination-mediated cassette exchangeDepositorInsertsgCR8
UseMouse TargetingAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRP(Exp)-CMV> ORF_924bp (Azgp1):dTomato:IRES:Puro
Plasmid#110831PurposeExpresses both zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter, where the fluorescent dT reporter is fused with the ZAG protein.DepositorAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Rheb(D60I)-IRES-eGFP-W
Plasmid#32521DepositorInsertDominant negative Rheb (D60I) (Rheb Rat)
UseLentiviralExpressionMammalianMutationD60I to make dominant negativePromoterCMVAvailable SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
mouse pGL3-ank3_1-promoter firefly luciferase
Plasmid#86436PurposeMouse pGL3-ank3_1-promoter firefly luciferase, ca 2000 base pairs around TSS exon 1eDepositorInsertank3_1 promoter
Tagsn/aExpressionMammalianMutationn/aPromoterankyrin-3_1Available SinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only