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Showing: 2011 - 2040 of 2136 results

  1. pcDNA3.1-α-COPI-WD40-Arg57Ala

    Plasmid
    #180759
    Purpose
    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg57Ala (residues 1-327)
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1
    Insert
    α-COPI-WD40-Arg57Ala
    Use
    Tags
    C-terminal strep tag
    Expression
    Mammalian
    Mutation
    Encodes residues 1-327; mutations: Arg57Ala, Leu1…
    Promoter
    CMV
    Available since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    180759-plasmid-map-sequence-id-356141

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  2. pcDNA3.1-α-COPI-WD40-Tyr139Ala

    Plasmid
    #180761
    Purpose
    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Tyr139Ala (residues 1-327)
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1
    Insert
    α-COPI-WD40-Tyr139Ala
    Use
    Tags
    C-terminal strep tag
    Expression
    Mammalian
    Mutation
    Encodes residues 1-327; mutations: Tyr139Ala, Leu…
    Promoter
    CMV
    Available since
    March 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    180761-plasmid-map-sequence-id-356140

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  3. pcDNA3.1-α-COPI-WD40-Asp115Ala

    Plasmid
    #180760
    Purpose
    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Asp115Ala (residues 1-327)
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1
    Insert
    α-COPI-WD40-Asp115Ala
    Use
    Tags
    C-terminal strep tag
    Expression
    Mammalian
    Mutation
    Encodes residues 1-327; mutations: Asp115Ala, Leu…
    Promoter
    CMV
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    180760-plasmid-map-sequence-id-356107

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  10. pSems leader-ALFA-mXFP-TpoR

    Plasmid
    #222945
    Purpose
    Expression of TPOR with ALFAtag and monomeric XFP in mammalian cells
    Depositor
    Jacob Piehler
    Article
    Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:
    Insert
    Thrombopoietin receptor (26-635) (MPL Human)
    Use
    Tags
    ALFAtag, Ig k-chain leader sequence, and mXFP
    Expression
    Mammalian
    Mutation
    mXFP: tryptophan 66 to phenylalanine, TPOR: Signa…
    Promoter
    CMV
    Available since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    222945-plasmid-map-sequence-id-457424

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  12. pAAV-CMV-CASANOVA

    Plasmid
    #113036
    Purpose
    AAV vector for CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9
    Depositor
    Dominik Niopek
    Article
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    Use
    AAV, CRISPR, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    113036-plasmid-map-sequence-id-226953

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  13. RRAGC

    Plasmid
    #25335
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RRAGC:S60-I237 (RRAGC Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids S60-I237
    Promoter
    Available since
    Aug. 17, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

    Plasmid
    #182653
    Purpose
    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    mutant eukaryotic release factor 1
    Use
    Tags
    HA tag and nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    E55D and Y306A/Y384F (AF) double mutant of Methan…
    Promoter
    CMV and U6
    Available since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    182653-plasmid-map-sequence-id-363026

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  15. p2151 pHAGE-hSPC-TFEB-TagBFP-W

    Plasmid
    #188722
    Purpose
    Lentiviral vector allowing for SFTPC-driven expression of TFEB:tagBFP fusion protein
    Depositor
    Darrell Kotton
    Insert
    TFEB (TFEB Human)
    Use
    Lentiviral
    Tags
    tagBFP
    Expression
    Mutation
    Promoter
    hSP-C
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188722-plasmid-map-sequence-id-372785

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Showing: 2011 - 2040 of 2136 results