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Plasmid#64758PurposeRecombination-Induced Tag Exchange (RITE) Cassette, HA-6xHIS to a V5 epitope tag, Hygro selectionDepositorInsertRITE cassette
UseRite cassetteTagsExpressionMutationPromoternoneAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ shNS
Plasmid#127696PurposeDoxycyclin inducible pTRIPZ lentivirus vector containing a non-silencing sequence for both human and mouseDepositorInsertshNS
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSin Capsid
Plasmid#127693PurposePlasmid contains the Sindbis capsid gene and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.DepositorInsertSindbis Capsid
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 3, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
HA-HIF1alpha-pcDNA3
Plasmid#18949DepositorInsertHypoxia inducible factor 1 alpha (HIF1A Human)
UseTagsHA-tagExpressionMammalianMutationwt cDNAPromoterAvailable sinceSept. 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
Lenti-multi-Guide
Plasmid#85401PurposeLentiviral vector for the delivery of multiple sgRNAs targeting different genes in combination with inducible Cas9 expresssion by pLenti-iCas9-neoDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTXB1.Tat(48-57)-Cre recombinase
Plasmid#165042PurposeExpression of Tat(48-57) conjugated Cre recombinase for protein purification (Intein-chitin binding domain fussion)DepositorInsertCre recombinase
UseTagsMxe Intein-Chitin binding domain and Tat(48-57)ExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-DelGFP
Plasmid#133302PurposeEGFP reporter was removed in the TLCV2 vector so it could be used for inducible gene CRISPR knockout in cell lines with an eGFP reporter.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-EF1-IRES-NEO deleted ITR
Plasmid#67906PurposePiggyBac transposon encoding Neomycin resistance with deleted inverted terminal repeatDepositorInsertNeoR (neoR Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2alpha-pcDNA3
Plasmid#18950DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseTagsHA-tagExpressionMammalianMutationwt cDNAPromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBA-mCherry-EGFP-PIM
Plasmid#111758PurposeLow level expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 additionDepositorInsert2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP
UseTagsExpressionMammalianMutationVal24Glu, Tyr80Cys, Ala95Thr mutations in second …Promoterchicken beta-actinAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Cas9_Blast
Plasmid#125592PurposeLentiviral expression of spCas9 with blasticidin resistance geneDepositorInsertCas9
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterEFS promoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synapsin-HaloCaMP1a-EGFP
Plasmid#138327Purposechemigenetic calcium indicatorDepositorHas ServiceAAV1 and AAV9InsertHaloCaMP1a
UseAAVTagsEGFP, His-tag, and Nuclear Exclusion SignalExpressionMammalianMutationPromotersynapsinAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
MT1E
Plasmid#105702PurposeBacterial expression plasmids for production of recombinant metallothionein 1eDepositorInsertMetallothionein 1e (MT1E Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGW1-mCherry-EGFP-PIM
Plasmid#111759PurposeHigh expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 additionDepositorInsert2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP
UseTagsExpressionMammalianMutationVal24Glu, Tyr80Cys, Ala95Thr mutations in second …PromoterCMVAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-mC-G-LC3B-P
Plasmid#124974Purposeevaluate autophagyDepositorInsertmCherry-GFP-LC3B
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
YE1-BE4max
Plasmid#138155PurposeC-to-T base editorDepositorInsertrAPOBEC1(YE1)-nSpCas9-UGI-UGI
UseTagsNLSExpressionMammalianMutationWithin rAPOBEC1 W90Y + R126E; within Cas9 D10APromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB_tre_Cas9
Plasmid#126029PurposePiggyBac cargo vector expressing doxycycline inducible Cas9DepositorInsertshumanized S. pyrogenes Cas9
Hygromycin resistance
UseCRISPR; PiggybacTagsExpressionMammalianMutationPromoterEF1 alpha and TREAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synapsin-HaloCaMP1b-EGFP
Plasmid#138328Purposechemigenetic calcium indicatorDepositorHas ServiceAAV1 and AAV9InsertHaloCaMP1b
UseAAVTagsEGFP, His-tag, and Nuclear Exclusion SignalExpressionMammalianMutationPromotersynapsinAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
hisSUMO-CTCF(F1-F9)
Plasmid#102859PurposeExpress DNA binding domain of mouse CTCF in E. coliDepositorInsertCTCF (Ctcf Mouse)
UseTagshisSUMOExpressionBacterialMutationPromoterT7Available sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/HA-FLAG-RBP2
Plasmid#14800DepositorInsertRetinoblastoma Binding Protein 2 (KDM5A Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJM220 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD)
Plasmid#110559PurposeminiTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoterDepositorInsertrhaSR-PrhaBAD
UseTagsExpressionBacterialMutationPromoterrhaSR-PrhaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(7FTrp)RS
Plasmid#177310PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFR-mScarletI-antiFRET-P2A-G-Ca-FLITS
Plasmid#191457PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) G-Ca-FLITS to mScarlet-I fluorescent proteinDepositorInsertG-Ca-FLITS
UseTagsHis-mScarletI-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRY2PHR-mCherryN1
Plasmid#26866PurposeExpresses CRY2(1-498)-mCherry fusion for use in light-inducible protein interaction modulesDepositorInsertCRY2PHR (CRY2 Mustard Weed)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-MCS
Plasmid#84008Purposedoxycycline-inducible lentiviral vectorDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterTRE-CMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
TRE-DIO-eYFP
Plasmid#117383PurposeAn AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFPDepositorHas ServiceAAV1InsertEYFP
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-BLU-VIPR
Plasmid#220497PurposeRetroviral plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222-2A-Thy1.1 and the C120 element followed by cloning sites for ribozyme flanked gRNA (RGR).DepositorTypeEmpty backboneUseCRISPR and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBLU-VIPR
Plasmid#220498PurposePlasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).DepositorTypeEmpty backboneUseCRISPR; TransposonTagsExpressionMammalianMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
piGECI-N1
Plasmid#160421PurposeCytoplasmic expression of a near-infrared genetically encoded calcium indicator iGECIDepositorInsertiGECI
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Pam-FL
Plasmid#42570DepositorInserthuman MYCBP2 (MYCBP2 Human)
UseTagsMycExpressionMammalianMutationPam-FL clone lacks alternatively spliced exons 52…PromoterCMVAvailable sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPV-EF1a-EGxxFP-iP-A
Plasmid#204624PurposeCloning vector for SSA vector (EGxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TB:5xNF- κβ_RE:MiniP
Plasmid#133878Purpose5 copies of the NF-κβ DNA binding motifDepositorInsert5 copies of the NF-kb DNA binding motif
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Profiline
Plasmid#89925PurposeBinds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actinDepositorInsertprofiline-1 (PFN1 Human)
UseTagsNoneExpressionBacterialMutationPromoterAvailable sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJM101(pUC18T-miniTn7T-gm-lacIq-Ptac)
Plasmid#110558PurposeminiTn7 delivery plasmid with lacIq-Ptac inducible promoterDepositorInsertlacIq-Ptac
UseTagsExpressionBacterialMutationPromoterlacIq-PtacAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GT_AIO-TetOn_Divergent Donor
Plasmid#229784PurposeBxb1-GT donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)DepositorInsertLacZ; rtTA-T2A-mTagBFP2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE and CAGAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA_AIO-TetOn_Divergent Donor
Plasmid#229786PurposeBxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)DepositorInsertLacZ; rtTA-T2A-mTagBFP2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE and CAGAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
Puro-Cas9 donor
Plasmid#58409PurposeDonor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locusDepositorInserthSpCas9
UseCRISPRTags3xFlag, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-Mitotrap
Plasmid#46942PurposeExpresses Mitotrap in mammalian cellsDepositorInsertMitotrap (MTOR Aequorea victoria, Synthetic, Budding Yeast, Human)
UseTagsThis is a fusion between a yeast mitochondrial ta…ExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-mR7BD
Plasmid#79149Purposebacterial expression of GST-mR7BD (Rab7 binding domain of murine RILP)DepositorInsertR7BD (Rilp Mouse)
UseTagsGSTExpressionBacterialMutationamino acids 220-299PromotertacAvailable sinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_LKB1
Plasmid#108111PurposeLentiviral expression plasmid of human LKB1 cDNA with neomycin resistance geneDepositorInsertLKB1 (STK11 Human)
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterEFS promoterAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMT1420
Plasmid#228316PurposeHomoserine lactone (HSL)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterHomoserine lactone (HSL)-regulatable synthetic pr…Available sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT950
Plasmid#228290Purpose2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoter2,4-Diacetylphloroglucinol-regulatable synthetic …Available sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDN77
Plasmid#61347PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkBα NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-biNLS2 and NES IkBaExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHP138 Myc-TRF2-deltaB deltaM
Plasmid#164255Purposefor generation of mammalian cells with inducible expression of dominant negative TRF2 for induction of telomere crisis used together with any PiggyBac transposase plasmidDepositorInsertTRF2
UseTagsMycExpressionMammalianMutationdeletion of amino acids-86 and 496-542PromoterTetracycline inducible promotorAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA1-GATA6
Plasmid#51929Purposemammalian expression of mouse GATA6DepositorInsertGATA6 (Gata6 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
AF1521-c002
Plasmid#173109PurposeExpression of recombinant fusion protein in E. coliDepositorInsertAF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))
UseTagsmCeruleanExpressionBacterialMutationKH-M1-L192PromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT448
Plasmid#139388PurposeBile Acid inducible sgRNA-1 with PM1 driving Nanoluc expression (NOT Gate 1), pNBU1 backbone, AmpR, ErmRDepositorInsertBile Acid inducible sgRNA-1 with PM1 driving Nanoluc expression (NOT Gate 1),
UseSynthetic BiologyTagsExpressionMutationPromoterBile Acid inducible PromoterAvailable sinceJan. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT450
Plasmid#139390PurposeBile Acid inducible sgRNA-3 with PM3 driving Nanoluc expression (NOT Gate 3), pNBU1 backbone, AmpR, ErmRDepositorInsertBile Acid inducible sgRNA-3 with PM3 driving Nanoluc expression (NOT Gate 3), pNBU1 backbone, AmpR, ErmR
UseSynthetic BiologyTagsExpressionMutationPromoterBile Acid inducible PromoterAvailable sinceJan. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT492
Plasmid#139397PurposeBile Acid inducible sgRNA-5 with PM5 driving Nanoluc expression (NOT Gate 5), pNBU1 backbone, AmpR, ErmRDepositorInsertBile Acid inducible sgRNA-5 with PM5 driving Nanoluc expression (NOT Gate 5)
UseSynthetic BiologyTagsExpressionMutationPromoterBile Acid inducible PromoterAvailable sinceJan. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT493
Plasmid#139398PurposeBile Acid inducible sgRNA-6 with PM6 driving Nanoluc expression (NOT Gate 6), pNBU1 backbone, AmpR, ErmRDepositorInsertBile Acid inducible sgRNA-6 with PM6 driving Nanoluc expression (NOT Gate 6)
UseSynthetic BiologyTagsExpressionMutationPromoterBile Acid inducible PromoterAvailable sinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only