We narrowed to 5,538 results for: pcas

1. pCAG_WIPI2d-TEV-GST

   Plasmid

   #223799

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens, James Hurley

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   WIPI2d (WIPI2 Human)
   

   Use

   Tags

   TEV-GST

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG_GST-TEV-WIPI3

   Plasmid

   #223800

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens, James Hurley

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   WIPI3 (WDR45B Human)
   

   Use

   Tags

   GST-TEV

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG_GST-TEV-WIPI4

   Plasmid

   #223801

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens, James Hurley

   Article

   Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

   Insert

   WIPI4 (WDR45 Human)
   

   Use

   Tags

   GST-TEV

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG/TSF-VPS34

   Plasmid

   #232916

   Purpose

   Mammalian Expression

   Depositor

   James Hurley

   Insert

   VPS34 (PIK3C3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9 (BB)-2A-GFP-RB1sg

   Plasmid

   #229857

   Purpose

   single guide RNA targeting human RB1 gene

   Depositor

   Maria Alvarado-Kristensson

   Article

   Lindstrom et al FASEB J. 2025 Mar 15;39(5):e70431. doi:

   Insert

   RB1sgRNA (RB1 Human)
   

   Use

   Tags

   The gamma-tubulin sgRNA is coexpressed with 3xFLA…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas9-EcRT-Z3-Nat

   Plasmid

   #232094

   Purpose

   Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

   Insert

   Z3 promoter and Z3EV transcription factor
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Z3

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST-CMV-EGFPCAAX

   Plasmid

   #232140

   Purpose

   Expresses EGFP with a CAAX motif under control of a CMV promoter

   Depositor

   Steven Farber

   Article

   Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

   Insert

   EGFPCAAX
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCas9-EcRT-Z3-Kan

   Plasmid

   #232096

   Purpose

   Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

   Insert

   Z3 promoter and Z3EV transcription factor
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Z3

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. aTY135_mtk02_pCAG-nfGFPtether_P2A_H2B-IRFP_SV40pA

   Plasmid

   #225350

   Purpose

   piggyback backbone for non-fluorescent GFP tether and H2B-IRFP expression

   Depositor

   Wendell Lim

   Article

   Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

   Insert

   H2B (H2B Human, Synthetic)
   

   Use

   Tags

   miRFP and non-fluorescent GFP (Y67F)-P2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCA528 IST1 (1-366)

    Plasmid

    #193039

    Purpose

    Bacterial expression plasmid for full-length IST1 (1-366).

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    IST1 (IST1 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-CAPN7-OSF

    Plasmid

    #193041

    Purpose

    Mammalian expression plasmid encoding full-length CAPN7 with C-terminal OSF tag (One-Strep-FLAG)

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    OSF (One-Strep-FLAG)

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta-actin

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCA528 CAPN7 (81-165)

    Plasmid

    #193032

    Purpose

    Bacterial expression plasmid for CAPN7 (81-165) C-terminal MIT domain

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    Residues 81-165

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-nucGFP11-3xPax7gRNA

    Plasmid

    #224570

    Purpose

    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    GFP11-H2B
    

    Use

    CRISPR

    Tags

    Histone H2B (nuclear localization)

    Expression

    Mutation

    Codon optimized

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCambia3301-AtU6-MAR1

    Plasmid

    #210757

    Purpose

    Mutagenesis of the Arabidopsis MAR1 gene for efficient screening.

    Depositor

    Daisuke Miki

    Article

    Cheng et al Sci Rep. 2024 Dec 28;14(1):30791. doi: 1

    Insert

    AtU6-26 pro::MAR1 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6-26

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCherry-pCAG-mock

    Plasmid

    #228504

    Purpose

    This plasmid can be used for control experiments and can also be used as a plasmid backbone

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-protein-mock

    Plasmid

    #228505

    Purpose

    This plasmid can be used for control experiments and can also be used as a plasmid backbone

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCASCADE-LCAmp-trxB

    Plasmid

    #202465

    Purpose

    Expresses a guide RNA to silence trxB after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    trxB guide RNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ugpB

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-BiPOLES-mRuby3

    Plasmid

    #227780

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons fused to mRuby3

    Depositor

    Hillel Adesnik

    Insert

    BiPOLES-mRuby3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-TSF-TBK1

    Plasmid

    #216709

    Purpose

    Mammalian expression of TBK1

    Depositor

    James Hurley

    Article

    Tudorica et al J Cell Biol. 2024 Jul 1;223(7):e20230901

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Tags

    TSF

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. SpCas9-NRCH-CGBE1

    Plasmid

    #198557

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NRCH-CGBE1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-CGBE1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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