We narrowed to 10,732 results for: Kars
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Plasmid#202462PurposeExpresses redox sensitive GFP (roGFP) after phosphate depletionDepositorInsertreduction-oxidation sensitive green fluorescent protein
UseTagsExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAP007
Plasmid#206809PurposepET-6xHis-MBP-TEV-BabTIR-APAZ/BabAgo - Heterologous BabSPARTA expressionDepositorInsertBabSPARTA operon (6xHis-MBP-BabTIR-APAZ and BabAgo)
UseTags6xHis-MBPExpressionBacterialMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRetro-UMI
Plasmid#222686PurposeCRISPR-screeningDepositorTypeEmpty backboneUseCRISPR and RetroviralTagsExpressionMammalianMutationPGK without BbsI cutsitePromoterAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330_NPM2_cterm1
Plasmid#222911PurposeCas9/sgRNA plasmid for targeting NPM2DepositorInsertCas9, NPM2 sgRNA 1 (NPM2 Synthetic, Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330_NPM2_cterm4
Plasmid#222914PurposeCas9/sgRNA plasmid for targeting NPM2DepositorInsertCas9, NPM2 sgRNA 4 (NPM2 Synthetic, Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330_NPM2_cterm3
Plasmid#222913PurposeCas9/sgRNA plasmid for targeting NPM2DepositorInsertCas9, NPM2 sgRNA 3 (NPM2 Synthetic, Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330_NPM2_cterm2
Plasmid#222912PurposeCas9/sgRNA plasmid for targeting NPM2DepositorInsertCas9, NPM2 sgRNA 2 (NPM2 Synthetic, Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBL7086
Plasmid#167250Purposeorthogonal Mango reporter output for cell-free expressionDepositorInsertpAKSIRV-Mango
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK6031
Plasmid#219739PurposeMoClo-compatible Level 0 - promoter + 5U vector carrying promoter CmYLCVDepositorInsertCestrum yellow leaf curling virus (CmYLCV) promoter
UseSynthetic BiologyTagsExpressionPlantMutationPromoterCmYLCVAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-GST-TEV-NAP1
Plasmid#217610PurposeFor expression in E. coli of NAP1DepositorInsertNAP1 (AZI2 Human)
UseTagsGSTExpressionBacterialMutationPromoterTAC promoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Dual-GST-TEV-SINTBAD-mCherry
Plasmid#208874PurposeFor expression in insect cells of SINTBAD-mCherryDepositorInsertSINTBAD (TBKBP1 Human)
UseTagsGST-TEVExpressionInsectMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-h.Rhno1 Plk1mut7A-C-PAMmut-Myc-DDK-P2A-Puro
Plasmid#211616PurposeRhno1 Plk1mut7ADepositorInserthRHNO1 (RHNO1 Human)
UseTagsmycExpressionMammalianMutationS51A, T52A, S141A, S162A, S163A, S164A, S178APromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
GfaABC1D-Crym-eGFP
Plasmid#200080PurposeTo study u-crystallin protein interactors, control plasmidDepositorInsertGfaABC1D-Crym-eGFP
UseAAV and Mouse TargetingTagseGFPExpressionMutationPromoterGfaABC1DAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
hLig4 guide2 in pX458
Plasmid#211536PurposesgRNA-2 against Lig4DepositorInsertsgRNA hLig4 (LIG4 Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoteru6Available sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
hLig4 guide1 in pX458
Plasmid#211535PurposesgRNA-1 against Lig4DepositorInsertsgRNA hLig4 (LIG4 Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoteru6Available sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-h.Rhno1 delta TOPBP1 (T202A)-C-Myc-P2A-Puro
Plasmid#211619PurposeRhno1 delta TOPBP1 (T202A)DepositorInserthRHNO1 (RHNO1 Human)
UseTagsmyc-flagExpressionMammalianMutationT202APromoterCMVAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSSA-DsRed/eGFP (pXK-442)
Plasmid#208827PurposeThe SSA-based surrogate reporter for validating the activity of different gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CMV-DsRed-CMV-eGFP*(SSA).DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSSA-eGFP (pXK-221)
Plasmid#208826PurposeThe SSA-based surrogate reporter for validating the activity of gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(SSA).DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmcb306-GFP-Rab12-mito
Plasmid#207349PurposeMammalian expression of Rab12-mitoDepositorInsertRab12-mito (RAB12 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
p35S::SP-mCherry-GFP-HDEL
Plasmid#159097PurposePositive control for FRET in plant ER / nuclear envelope.DepositorInsertSP-mCherry-GFP-HDEL
UseTagsmCherry, GFPExpressionPlantMutationPromoter35SAvailable sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
MLKS2delARMec
Plasmid#130860PurposeExpression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biologyDepositorInsertMLKS2
UseGateway entry vectorTagseGFP-FLAG-HAExpressionMutationDeletion of armadillo domainPromoterAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKS2del4exp
Plasmid#130861PurposeExpression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biologyDepositorInsertMLKS2
UseTagseGFP-FLAG-HAExpressionPlantMutationDeletion of terminal four amino acidsPromoterAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKS2delKASHexp
Plasmid#130862PurposeExpression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biologyDepositorInsertMLKS2
UseTagseGFP-FLAG-HAExpressionPlantMutationDeletion of KASH domainPromoterAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKS2delTMexp
Plasmid#130863PurposeExpression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biologyDepositorInsertMLKS2
UseTagseGFP-FLAG-HAExpressionPlantMutationDeletion of transmembrane domainPromoterAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKS2delARMexp
Plasmid#130864PurposeExpression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biologyDepositorInsertMLKS2
UseTagseGFP-FLAG-HAExpressionPlantMutationDeletion of armadillo domainPromoterAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKS2del4ec
Plasmid#130857PurposeExpression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biologyDepositorInsertMLKS2
UseGateway entry vectorTagseGFP-FLAG-HAExpressionMutationDeletion of terminal four amino acidsPromoterAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
MLKS2delKASHec
Plasmid#130858PurposeExpression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biologyDepositorInsertMLKS2
UseGateway entry vectorTagseGFP-FLAG-HAExpressionMutationeGFP-FLAG-HAPromoterAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX-2T_RV-B14_VP1
Plasmid#202074PurposeBacterial expression of VP1 protein from rhinovirus B14 (RV-B14). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertRV-B14 VP1 protein with Histag
UseTags6xHis-tag and GSTExpressionBacterialMutationPromotertacAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDropIn-T2A-ZpGal4DBD
Plasmid#199199PurposeInsert to create ZpGal4DBD knock-ins, via Drop-In cloning, released via BsmBI digestDepositorInsertT2A-ZpGal4DBD
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDropIn-T2A-p65Zp
Plasmid#199200PurposeInsert to create p65Zp knock-ins, via Drop-In cloning, released via BsmBI digestDepositorInsertT2A-p65Zp
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNMSB88
Plasmid#199321PurposeCombine with Gal4 to direct TeNL expressionDepositorInsert15xUAS::delta pes-10::::TeNL::let-858 3'UTR
UseTagsExpressionWormMutationKD for calcium is 250 nM, 1 synthetic intronPromoter15xUAS::delta pes-10Available sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNMSB109
Plasmid#199322PurposeCombine with Gal4 to direct CaMBI 300 expressionDepositorInsert15xUAS::delta pes-10::::CaMBI::let-858 3'UTR
UseTagsExpressionWormMutationOrange CaMBI with 300 nM KD for calciumPromoter15xUAS:::delta pes-10Available sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCitro-15a
Plasmid#202033Purposep15a-based plasmid that encodes a conjugative system synthesized from metagenomic data for conjugation to CitrobacterDepositorInsertconjugative system from a Citrobacter spp.
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_RV-A89_VP1
Plasmid#202073PurposeBacterial expression of VP1 protein from rhinovirus A89 (RV-A89). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertRV-A89 VP1 protein with Histag
UseTags6xHis-tag and GSTExpressionBacterialMutationPromotertacAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_E30_VP1
Plasmid#202070PurposeBacterial expression of VP1 protein from echovirus 30 (E30). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertE30 VP1 protein with Histag
UseTags6xHis-tag and GSTExpressionBacterialMutationPromotertacAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_CVB1_VP1
Plasmid#202069PurposeBacterial expression of VP1 protein from coxsackievirus B1 (CVB1). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertCVB1 VP1 protein with Histag
UseTags6xHis-tag and GSTExpressionBacterialMutationPromotertacAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_CVA4_VP1
Plasmid#202068PurposeBacterial expression of VP1 protein from coxsackievirus A4 (CAV4). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertCVA4 VP1 protein with Histag
UseTags6xHis-tag and GSTExpressionBacterialMutationPromotertacAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pminiTol2-4kb wnt3 promoter-PMTmApple
Plasmid#203794PurposeTol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of PMTmApple for zebrafish transgenesisDepositorInsertPMT
UseZebrafish expressionTagsmAppleExpressionMutationPromoter4kb Wnt3 promoterAvailable sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR-TC9
Plasmid#202088Purposeentry vector for gateway recombination of TevCas9 and sgRNA into a destination cassette in pCitro-destDepositorInsertTevCas9 + sgRNA cassette
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRa_CHO-Bip_gRNA1
Plasmid#183694PurposeCRISPRa gRNA expression vector for Chinese Hamster Bip (Hspa5)DepositorInsertgRNA targeting chinese hamster Bip (Hspa5)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF_641-1574_AH
Plasmid#148936PurposeInsect Expression of DmGIGYF1_641-1574DepositorInsertDmGIGYF1_641-1574 (Gyf Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF-F361AF367A_AH
Plasmid#148937PurposeInsect Expression of DmGIGYF-F361AF367ADepositorInsertDmGIGYF-F361AF367A (Gyf Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF-W349A_AH
Plasmid#148938PurposeInsect Expression of DmGIGYF-W349ADepositorInsertDmGIGYF-W349A (Gyf Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF-WFFtoAAA_AH
Plasmid#148939PurposeInsect Expression of DmGIGYF-WFFtoAAADepositorInsertDmGIGYF-WFFtoAAA (Gyf Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF-YYMYFWFtoAAAAAAA_AH
Plasmid#148941PurposeInsect Expression of DmGIGYF1-YYMFWFtoAAAAAAADepositorInsertDmGIGYF1-YYMFWFtoAAAAAAA (Gyf Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHPat-PPFPPFtoAAAAAA_AH
Plasmid#148908PurposeInsect Expression of DmHPat-PPFPPFtoAAAAAADepositorInsertDmHPat-PPFPPFtoAAAAAA (Patr-1 Fly)
UseTagsExpressionInsectMutationthree silent mutations compared to the sequence g…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4EHP-W114A_AH
Plasmid#148909PurposeInsect Expression of Dm4EHP-W114ADepositorInsertDm4EHP-W114A (4EHP Fly)
UseTagsExpressionInsectMutation4 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF-del331-370_AH
Plasmid#148911PurposeInsect Expression of DmGIGYF-del331-370DepositorInsertDmGIGYF-del331-370 (Gyf Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGIGYF-YFWFtoAAAA_AH
Plasmid#148913PurposeInsect Expression of DmGIGYF-YFWFtoAAAADepositorInsertDmGIGYF-YFWFtoAAAA (Gyf Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-Dm4EHP-R93PE139L_AH
Plasmid#148915PurposeInsect Expression of Dm4EHP-R93PE139LDepositorInsertDm4EHP-R93PE139L (4EHP Fly)
UseTagsExpressionInsectMutation4 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only