We narrowed to 1,406 results for: PTS;

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HA-doraWT_rescue_construct

Plasmid

#190608

Purpose

Puromycin-selectable expression of HA-tagged Dora (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Tags

3xHA

Expression

Insect

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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psgRNA-2xMS2-mSAT

Plasmid

#181908

Purpose

Single guide RNA with 2XMS2 loops targeting mouse major satellite repeats

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

sgRNA-2XMS2-mSAT

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6Biso1_C

Plasmid

#146087

Purpose

Mammalian Expression of HsTNRC6Ciso1

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6Ciso1 (TNRC6C Human)

Expression

Mammalian

Mutation

two silent and three non silent mutations, S337G,…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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p198 Dync1i2.F (Ex1a)

Plasmid

#26448

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 2 isoform F (exon 1a) (Dync1i2 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

March 24, 2011

Availability

Academic Institutions and Nonprofits only
pGenLenti V5-NIX (E72A/L75A/D77A/E81A; deltaWIPI2)

Plasmid

#223789

Purpose

Stable expression of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

NIX (BNIP3L) (BNIP3L Human)

Use

Lentiviral

Tags

V5

Mutation

E72A/L75A/D77A/E81A; disrupts WIPI2 binding

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-MBP-TEV-NAP1_delta-NDP52 (S37K/A44E)

Plasmid

#208872

Purpose

For expression in E. coli of NAP1 (delta-NDP52)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

MBP-TEV

Expression

Bacterial

Mutation

S37K/A44E (disrupts NDP52 binding)

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-MBP-TEV-NAP1_delta-TBK1 (L226Q/L233Q)

Plasmid

#208873

Purpose

For expression in E. coli of NAP1 (delta-TBK1)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

MBP-TEV

Expression

Bacterial

Mutation

L226Q/L233Q (disrupts TBK1 binding)

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pVH269-Tier1-OVanO2-PCMVmin2-SEAP-p2A-iRFP670

Plasmid

#169667

Purpose

Tier-1 vector encoding PVanO2-driven SEAP-p2A-iRFP670 expression (OVanO2-PCMVmin-2-SEAP-p2A-iRFP670-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

vanillic acid-controlled SEAP and iRFP expression

Expression

Mammalian

Promoter

VanO2-PCMVmin

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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CMV EGFP-PPM1H-H153D

Plasmid

#200432

Purpose

Mammalian expression of H153D mutation in PPM1H

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

PPM1H protein phosphatase, Mg2+/Mn2+ dependent 1H

Tags

EGFP

Expression

Mammalian

Mutation

H153D

Promoter

CMV

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-C-term_F

Plasmid

#146294

Purpose

Mammalian Expression of HsTNRC6A-Cterm

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6A-Cterm (TNRC6A Human)

Expression

Mammalian

Mutation

one non silent mutation K1578E compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-delAGOhook_F

Plasmid

#146295

Purpose

Mammalian Expression of HsTNRC6A-delAGOhook

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6A-delAGOhook (TNRC6A Human)

Expression

Mammalian

Mutation

one non silent mutation K1578E compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-delMI_F

Plasmid

#146296

Purpose

Mammalian Expression of HsTNRC6A-delMI

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6A-delMI (TNRC6A Human)

Expression

Mammalian

Mutation

one non silent mutation K1578E compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-delMII_F

Plasmid

#146297

Purpose

Mammalian Expression of HsTNRC6A-delMII

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsTNRC6A-delMII (TNRC6A Human)

Expression

Mammalian

Mutation

one non silent mutation K1578E compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGFP-nE-mHP1alpha

Plasmid

#181895

Purpose

Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

nE-mHP1alpha (Cbx5 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

S11E, S12E, S13E, S14E

Promoter

CMV

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG

Plasmid

#200798

Purpose

Mammalian expression of CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al iScience. 2026 Feb 2;29(3):114875. doi:

Insert

KIF5A (Kif5a Mouse)

Tags

CLIPf and SNAPf, HisTag, FLAG

Expression

Mammalian

Mutation

411-680

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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dora[B]_rescue_construct

Plasmid

#190637

Purpose

Puromycin-selectable expression of Dora[T295M] (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Expression

Insect

Mutation

Changed threonine 295 to methionine

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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HA-dora[B]_rescue_construct

Plasmid

#190638

Purpose

Puromycin-selectable expression of HA-tagged Dora[T295M] (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Tags

3xHA

Expression

Insect

Mutation

Changed threonine 295 to methionine

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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dora[A]_rescue_construct

Plasmid

#190639

Purpose

Puromycin-selectable expression of truncated Dora (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Expression

Insect

Mutation

Truncated to contain amino acids 1-945

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-FUSN-mHP1alpha

Plasmid

#181897

Purpose

Fluorescently tagged HP1alpha fused to the N-terminal domain of the fused in sarcoma protein (FUSN)

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

FUSN-mHP1alpha (Cbx5 Mouse, Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-PGL-1-mHP1alpha

Plasmid

#181899

Purpose

Fluorescently tagged HP1alpha fused to PGL-1

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

PGL-1-mHP1alpha (Cbx5 Mouse, Nematode)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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