We narrowed to 18,512 results for: Tec

1. pcDNA3 PM-Ras-LOCKR-S

   Plasmid

   #216498

   Purpose

   FRET-based reporter for Ras activity, plasma membrane localized

   Depositor

   David Baker

   Article

   Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

   Insert

   PM-Ras-LOCKR-S
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PX854

   Plasmid

   #62886

   Purpose

   C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5).

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa573-1368)
   

   Use

   CRISPR

   Tags

   FKBP12, NLS SV40, and NLS nucleoplasmin

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3 Ras-LOCKR-S

   Plasmid

   #216496

   Purpose

   FRET-based reporter for Ras activity, untargeted

   Depositor

   David Baker

   Article

   Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

   Insert

   Ras-LOCKR-S
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJBL701

   Plasmid

   #140371

   Purpose

   Expresses TetR protein under the control of T7-lacO promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-lacO-TetR-T
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3 Golgi-Ras-LOCKR-S

   Plasmid

   #216499

   Purpose

   FRET-based reporter for Ras activity, Golgi localized

   Depositor

   David Baker

   Article

   Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

   Insert

   Golgi-Ras-LOCKR-S
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3 ER-Ras-LOCKR-S

   Plasmid

   #216500

   Purpose

   FRET-based reporter for Ras activity, ER localized

   Depositor

   David Baker

   Article

   Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

   Insert

   ER-Ras-LOCKR-S
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3 Mito-Ras-LOCKR-S

   Plasmid

   #216501

   Purpose

   FRET-based reporter for Ras activity, mitochondria localized

   Depositor

   David Baker

   Article

   Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

   Insert

   Mito-Ras-LOCKR-S
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pETNb-nALFA

   Plasmid

   #159988

   Purpose

   For the protein expression with ALFA tag at N-terminus in E. coli

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Type

   Empty backbone

   Use

   Tags

   MVKYI-8histidine-ALFA tag-HRV3C protease cleavage…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFastBacDual with PA+Histag

   Plasmid

   #84870

   Purpose

   Baculovirus Expression of Bacillus anthracis protective antigen

   Depositor

   Russell Vance

   Article

   Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

   Insert

   PA
   

   Use

   Baculovirus

   Tags

   His

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Nov. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFBNb-cALFA

    Plasmid

    #159990

    Purpose

    For the protein expression with ALFA tag at C-terminus in insect cells

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Use

    Tags

    HRV3C protease cleavage site-ALFA tag-8histidine

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LV anti-FITC-LaG16-Y186F-T2A-BFP

    Plasmid

    #194931

    Purpose

    LV construct encoding Y186F mutant sNRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-sNRR(Y186F)-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LV anti-FITC-LaG16-Y186A-T2A-BFP

    Plasmid

    #194932

    Purpose

    LV construct encoding Y186A mutant sNRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-sNRR(Y186A)-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJBL7025

    Plasmid

    #128810

    Purpose

    Fluoride riboswitch-regulated catechol 2,3-dioxygenase

    Depositor

    Julius Lucks

    Article

    Thavarajah et al ACS Synth Biol. 2019 Dec 12. doi: 10.102

    Insert

    Fluoride riboswitch-regulated catechol 2,3-dioxygenase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LV anti-FITC-LaG16-R103A-T2A-BFP

    Plasmid

    #194935

    Purpose

    LV construct encoding R103A mutant sNRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-sNRR(R103A)-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. psAAVS1-T2

    Plasmid

    #60543

    Purpose

    GFP reporter for CRISPR/CAS9 and gRNA_AAVS1-T2 activities

    Depositor

    William Simonds

    Article

    Zhang et al J Biotechnol. 2014 Sep 2. pii: S0168-165

    Insert

    ProtoSpacer AAVS1-T2 (AAVS1 Human)
    

    Use

    CRISPR; Kan

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LV TRE-GFP-TMD-DLL1

    Plasmid

    #194936

    Purpose

    LV construct encoding GFP-TMD-DLL1 ligand under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette.

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    eGFP-(PDGFR)TMD-rDLL1 ICD (WT)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LV anti-FITC-LaG16-Y192A-T2A-BFP

    Plasmid

    #194933

    Purpose

    LV construct encoding Y192A mutant sNRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-sNRR(Y192A)-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJBL719

    Plasmid

    #140389

    Purpose

    Expresses QacR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-QacR-T
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJBL721

    Plasmid

    #140391

    Purpose

    Expresses TtgR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-TtgR-T
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pXL-CAG-ICeuI-Cerulean-2A-sHRPbNLG1

    Plasmid

    #79912

    Purpose

    piggyBac transposon vector, Cerulean-2A-sHRPbNLG1

    Depositor

    Joshua Sanes

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    Cerulean-2A-sHRPbNLG1
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pXL-BacII-CAG-Zeo-t2A-sHRPaNRX3beta

    Plasmid

    #79913

    Purpose

    piggyBac transposon vector, Zeocin-2A-sHRPaNRX3beta

    Depositor

    Joshua Sanes

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    Zeocin-t2A-sHRPbNRX3beta
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p426-SNR52p-gRNA.clr-2.Y-SUP4t

    Plasmid

    #89692

    Purpose

    gRNA for clr-2 locus

    Depositor

    Christian Hong

    Article

    Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

    Insert

    gRNA for clr-2 locus
    

    Use

    CRISPR; Grna

    Tags

    No

    Expression

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TALEN-ID12-T

    Plasmid

    #41479

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

    Insert

    TALEN-FokI nuclease backbone
    

    Use

    Tags

    3xFLAG and WT FokI nuclease domain

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TALEN-ID12-C

    Plasmid

    #41477

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

    Insert

    TALEN-FokI nuclease backbone
    

    Use

    Tags

    3xFLAG and WT FokI nuclease domain

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. FB401

    Plasmid

    #209130

    Purpose

    Multipartite assembly of the TU for afpA expression under the regulation of afpA promoter and terminator with the pyr4 auxotrophy marker pyr4 from T. reseei

    Depositor

    Jose Marcos

    Article

    Ropero-Pérez et al Curr Res Biotechnol 2024

    Insert

    PafpA:afpA:TafpA::Ppyr4:pyr4:Tpyr4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. FB406

    Plasmid

    #203627

    Purpose

    Promoter of the TAKA-amylase A gene from A. oryzae, maltose/starch-inducible.

    Depositor

    Jose Marcos

    Article

    Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

    Insert

    PamyB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FB407

    Plasmid

    #203628

    Purpose

    Promoter of the elongation factor 1-alpha gene from P. digitatum (PDIG_59570).

    Depositor

    Jose Marcos

    Article

    Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

    Insert

    Pef1A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. FB408

    Plasmid

    #203629

    Purpose

    Promoter of the ubiquitin ligase gene from P. digitatum (PDIG_07760).

    Depositor

    Jose Marcos

    Article

    Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

    Insert

    P07760
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. FB367

    Plasmid

    #203630

    Purpose

    Module for the expression of geneticin resistance and Nanoluciferase.

    Depositor

    Jose Marcos

    Article

    Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

    Insert

    PgpdA:Nluc:Ttub::PtrpC:nptII:Ttub
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. FB273

    Plasmid

    #203612

    Purpose

    Terminator of pyr4 gene from T. reesei.

    Depositor

    Jose Marcos

    Article

    Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

    Insert

    Tpyr4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge