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Showing: 301 - 350 of 3206 results

  2. Lenti sgRNA NGFR GFP out of frame

    Plasmid
    #155282
    Purpose
    Lentiviral plasmid for sgRNA, NGFR marker, editing detected with GFP, used with 155280
    Depositor
    Martin Carroll
    Insert
    GFP out of frame IRES NGFR
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    155282-plasmid-map-sequence-id-310437

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  8. pLenti UBC MitoFlux (pSu9-HaloTag7-mGFP-STOP)

    Plasmid
    #215010
    Purpose
    Express a Mitophagy reporter in mammalian cells (untested)
    Depositor
    Wade Harper
    Insert
    pSu9 (Presequence of Fo-ATPase subunit 9)
    Use
    Lentiviral
    Tags
    HaloTag(7)-mGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    215010-plasmid-map-sequence-id-429275

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  11. lenti U6-sgRNA-acRNA SYN-dCas9-P2A-EGFP

    Plasmid
    #159086
    Purpose
    Expresses dCas9-P2A-EGFP driven by human SYN promoter and empty CRISPR Display sgRNA/accessory RNA from U6 promoter.
    Depositor
    Jeremy Day
    Article
    Carullo et al Nucleic Acids Res. 2020 Aug 18. pii: 589
    Insert
    empty crRNA-acRNA backbone, dCas9-P2A-EGFP
    Use
    CRISPR and Lentiviral
    Tags
    EGFP and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    hSYN, U6
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    159086-plasmid-map-sequence-id-310691

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  13. pLenti-FoxJ1 promoter-IRES-EGFP-Flag-hChibby1

    Plasmid
    #122964
    Purpose
    Expresses Flag-tagged Chibby1 in multiciliated cells under FoxJ1 promoter. It can be used as a cloning vector for gene of interest at SfiI sites.
    Depositor
    Ken-Ichi Takemaru, FENG-QIAN Li
    Insert
    human Chibby1 (CBY1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mutation
    Promoter
    FoxJ1 and human FoxJ1
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    122964-plasmid-map-sequence-id-291837

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  15. pLenti-PGK-c-myc-2A-CDK4(R24C)-GFP-Puro

    Plasmid
    #83107
    Purpose
    3rd generation lentiviral expression of C-myc, CDK4(R24C), and GFP separated by 2A self-cleaving peptide
    Depositor
    Andrew Brooks
    Inserts
    c-Myc (MYC Human)
    CDK4 (CDK4 Human)
    eGFP
    Use
    Lentiviral
    Tags
    2A self cleaving peptide
    Expression
    Mammalian
    Mutation
    R24C and p64 isoform
    Promoter
    hPGK and hPGK (single transcript)
    Available since
    July 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83107-plasmid-map-sequence-id-330062

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  16. ACE Reporter (pLenti-CMV-mCherry (+43) -T2A-eGFP)

    Plasmid
    #109428
    Purpose
    A lentiviral backbone expressing mCherry with a 43 basepair insert and eGFP off of a CMV promoter. A reporter for both Cas9 nuclease and APOBEC-mediated base-editing activity.
    Depositor
    Reuben Harris
    Article
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Insert
    mCherry T2A GFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Inserted 43 base pairs into mCherry to create rep…
    Promoter
    CMV
    Available since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    109428-plasmid-map-sequence-id-212894

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  20. pLenti UBC LC3Flux (HaloTag7-mGFP-LC3-STOP)

    Plasmid
    #215014
    Purpose
    Express an autophagy (LC3) reporter in mammalian cells (untested)
    Depositor
    Wade Harper
    Insert
    MAP1LC3B (Map1lc3b Rat)
    Use
    Lentiviral
    Tags
    HaloTag(7)-mGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    215014-plasmid-map-sequence-id-429267

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  25. U6-DNMT1-hSynapsin-PE2-Nterminal-P2A-EGFP-KASH-lenti

    Plasmid
    #135955
    Purpose
    Neuronal expression of N-terminal intein-split prime editor v2 (PE2) with EGFP-KASH marker and U6-mDNMT1 cassette. This plasmid is used by PE2, PE3, and PE3b
    Depositor
    David Liu
    Article
    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586
    Insert
    Prime editor v2 (PE2) N-terminal
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSynapsin
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    135955-plasmid-map-sequence-id-269512

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  26. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid
    #118158
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118158-plasmid-map-sequence-id-240404

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  31. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid
    #118162
    Purpose
    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118162-plasmid-map-sequence-id-240427

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  32. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide2

    Plasmid
    #118163
    Purpose
    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA2 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118163-plasmid-map-sequence-id-240400

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  34. pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPAC

    Plasmid
    #202717
    Purpose
    Mammalian co-expression of membrane-bound Pink Flamindo and soluble bPAC for cAMP perturbation and imaging
    Depositor
    Adam Cohen
    Article
    Xiang et al bioRxiv 2023
    Inserts
    Myr-Pink Flamindo
    EGFP-bPAC
    Use
    Lentiviral
    Tags
    10xHis and myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    202717-plasmid-map-sequence-id-402853

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  35. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide2

    Plasmid
    #118159
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA2 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118159-plasmid-map-sequence-id-240446

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  36. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide3

    Plasmid
    #118164
    Purpose
    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA3 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118164-plasmid-map-sequence-id-240433

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  37. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide3

    Plasmid
    #118160
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA3 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118160-plasmid-map-sequence-id-240351

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  39. pLenti-CAG-NOTCH2NL-delta C terminus (N2NL-delta C)-ires-EGFP

    Plasmid
    #122956
    Purpose
    Lentiviral expression of NOTCH2NL delta C terminus under CAG promoter with EGFP expression
    Depositor
    Pierre Vanderhaeghen
    Article
    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.
    Insert
    NOTCH2NL-delta C terminus (NOTCH2NLB Human)
    Use
    Lentiviral
    Tags
    6xhis tag
    Expression
    Mutation
    deletion of C terminus from full length NOTCH2NLB
    Promoter
    CAG promoter
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    122956-plasmid-map-sequence-id-244423

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  42. pLentiRNAGuide_004-hU6-RfxCas13d-DR1-BsmBI-EFS-EGFP-P2A-Puro-WPRE

    Plasmid
    #223175
    Purpose
    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.
    Insert
    hU6_Rfxcas13d-DR1_BsmBI
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    DR1 A>T
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    223175-plasmid-map-sequence-id-457165

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  43. pLentiX1-[TRE3G-EGFP-FMRP-bGH]-EFS-rtTA-P2A-mRuby2-WPRE

    Plasmid
    #235306
    Purpose
    Lentiviral expression of ComMAND base gene regulating EGFP-FMRP (therapeutically relevant gene)
    Depositor
    Kate Galloway
    Article
    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1
    Insert
    EGFP-Fmr1 (Fmr1 Mouse)
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G (3rd-generation Tet system)
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235306-plasmid-map-sequence-id-472748

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  44. pLenti-CAG-NOTCH2NL-delta EGF repeats (N2NL-delta EGF)-ires-EGFP

    Plasmid
    #122955
    Purpose
    Lentiviral expression of NOTCH2NL delta EGF repeats under CAG promoter with EGFP expression
    Depositor
    Pierre Vanderhaeghen
    Article
    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.
    Insert
    NOTCH2NL-delta EGF repeats (NOTCH2NLB Human)
    Use
    Lentiviral
    Tags
    6xhis tag
    Expression
    Mutation
    deletion of EGF repeats from full length NOTCH2NLB
    Promoter
    CAG promoter
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    122955-plasmid-map-sequence-id-244415

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  45. pLenti_EF1α-NES-Kinprola_PKA-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

    Plasmid
    #233371
    Purpose
    EF1α driven co-expression of the PKA activity recorder Kinprola_PKA fused to mEGFP and Cas9 expression through lentivirus transduction
    Depositor
    Kai Johnsson
    Article
    Sun et al bioRxiv 2024.09.11.611894
    Inserts
    NES-Kinprola_PKA-mEGFP
    FLAG-SV40NLS-Cas9-NLS-T2A-BSD
    Use
    Lentiviral
    Tags
    FLAG, NES, and mEGFP
    Expression
    Mutation
    Promoter
    EF1α
    Available since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    233371-plasmid-map-sequence-id-466776

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  46. pLentiX1-[TRE3G-EGFP(miRE.FF4)-FMRP-TS.FF6x1-bGH]-EFS-rtTA-P2A-mRuby2-WPRE

    Plasmid
    #235307
    Purpose
    Lentiviral expression of ComMAND open-loop circuit regulating EGFP-FMRP (therapeutically relevant gene)
    Depositor
    Kate Galloway
    Article
    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1
    Insert
    EGFP-Fmr1 (Fmr1 Mouse)
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G (3rd-generation Tet system)
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235307-plasmid-map-sequence-id-472801

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  47. pLentiX1-[TRE3G-EGFP(miRE.FF4)-FMRP-TS.FF4x1-bGH]-EFS-rtTA-P2A-mRuby2-WPRE

    Plasmid
    #235308
    Purpose
    Lentiviral expression of ComMAND closed-loop circuit regulating EGFP-FMRP (therapeutically relevant gene)
    Depositor
    Kate Galloway
    Article
    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1
    Insert
    EGFP-Fmr1 (Fmr1 Mouse)
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G (3rd-generation Tet system)
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235308-plasmid-map-sequence-id-472753

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  48. pLenti_EF1α-NES-Kinprola_on-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

    Plasmid
    #233373
    Purpose
    EF1α driven co-expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP and Cas9 expression through lentivirus transduction
    Depositor
    Kai Johnsson
    Article
    Sun et al bioRxiv 2024.09.11.611894
    Inserts
    NES-Kinprola_on-mEGFP
    FLAG-SV40NLS-Cas9-NLS-T2A-BSD
    Use
    Lentiviral
    Tags
    FLAG, NES, and mEGFP
    Expression
    Mutation
    Promoter
    EF1α
    Available since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    233373-plasmid-map-sequence-id-466778

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  49. pLenti_EF1α-NES-Kinprola_PKA_T/A-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

    Plasmid
    #233372
    Purpose
    EF1α driven co-expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA_T/A fused to mEGFP and Cas9 expression through lentivirus transduction
    Depositor
    Kai Johnsson
    Article
    Sun et al bioRxiv 2024.09.11.611894
    Inserts
    NES-Kinprola_PKA_T/A-mEGFP
    FLAG-SV40NLS-Cas9-NLS-T2A-BSD
    Use
    Lentiviral
    Tags
    FLAG, NES, and mEGFP
    Expression
    Mutation
    Promoter
    EF1α
    Available since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    233372-plasmid-map-sequence-id-466777

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Showing: 301 - 350 of 3206 results