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CMV-Brainbow-1.1 M

Plasmid

#18722

Depositor

Joshua Sanes

Article

Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

Insert

Kusabira ; M-mCherry ; M-mEYFP ; M-mCerulean

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pLKO‐puro‐2A‐mdx4cv‐GFP

Plasmid

#187053

Purpose

fluorescent reporter for adenine base editing of mdx4cv point mutation

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-p62-Silent_Mutation_WT

Plasmid

#187986

Purpose

human p62 with silent mutation for sip62 #5 (Thermo Scientific). Internal reference: SMC516

Depositor

Sascha Martens

Article

Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

Insert

p62 (NUP62 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

silent mutation for resistance to sip62 #5

Promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMBEC6

Plasmid

#187598

Purpose

Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Gentamycin resistance

Depositor

Pablo Ivan Nikel

Article

Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

Insert

nCas9-BEC-UGI; cas6f, gfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

trc

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 N-term RNPS1

Plasmid

#187574

Purpose

Express FLAG epitope and APEX2-tagged RNPS1 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

RNPS1 (RNPS1 Human)

Use

Tags

APEX2-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 C-term PML

Plasmid

#187583

Purpose

Express FLAG epitope and APEX2-tagged PML fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

PML (PML Human)

Use

Tags

APEX2-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV Myc T58A puro

Plasmid

#18773

Depositor

Scott Lowe

Article

Hemann et al Nature. 2005 Aug 11. 436(7052):807-11.

Insert

myc T58A (MYC Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

T58A

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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mCherry-p62-TIR-AAA

Plasmid

#187901

Purpose

Mammalian cell expression of a triple mutant of p62: P228A, E230A, P232A

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

SQSTM1 (SQSTM1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Triple alanine mutant: P228A, E230A, P232A

Promoter

CMV

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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AAV.hSyn.GLPLight-ctr

Plasmid

#187468

Purpose

Expresses GLPLight-ctr in mammalian cells

Depositor

Tommaso Patriarchi

Article

Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

Insert

GLPLight-ctr

Use

AAV

Tags

Flag tag

Expression

Mutation

Promoter

human Synapsin-1

Available since

Feb. 16, 2023

Availability

Academic Institutions and Nonprofits only

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UAS:TVA-mCherry

Plasmid

#187823

Purpose

Expression of a fusion of the transmembrane protein TVA and the red fluorescent protein mCherry under UAS control.

Depositor

Rainer Friedrich

Article

Satou et al Elife. 2022 Jul 22;11. pii: 77153. doi:

Insert

TVA-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

5xUAS

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pHRSin_hCD3δ-GFP2

Plasmid

#187355

Purpose

Lentiviral expression of truncated hCD3 delta-GFP2

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

Truncated hCD3 delta, and GFP2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pX601‐MHP1‐miniABEmaxNG‐N2‐(GG)

Plasmid

#187067

Purpose

Gp41-1 Split N-terminal half of ABEmaxNGA driven by MHP1 promoter with gRNA targeting mdx4cv nonsense mutation

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

miniABEmaxNG-Gp41

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

MHP1

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pX601‐MHP1‐ABEmaxC2‐NG‐E53 ogRNA

Plasmid

#187068

Purpose

Gp41-1 Split C-terminal half of ABEmaxNGA driven by MHP1 promoter with gRNA targeting mdx4cv nonsense mutation

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

Gp41-ABEmaxC2‐NG

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

MHP1

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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DL145_plenti_TRE_iPAK4

Plasmid

#187445

Purpose

Tet-On inducible expression of iPAK4 in a lentiviral backbone

Depositor

Adam Cohen

Article

Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

INKA-box-Pak4

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Pom121 GFP

Plasmid

#187680

Purpose

In vivo visualization of NPC protein Pom121

Depositor

Mary Beckerle

Insert

Pom121 (Pom121 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEX128·gRNA

Plasmid

#187600

Purpose

Template for amplification of gRNA with Cas6 recognition site

Depositor

Pablo Ivan Nikel

Article

Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

Insert

gRNA template

Use

Tags

Expression

Mutation

Promoter

no promoter

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV_hU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-Thy1.1

Plasmid

#187925

Purpose

sgRNA vector backbone expressing puromycin resistance and CD90.1 as selection markers

Depositor

Martin Turner

Article

Turner et al eLife. 2022 Apr 22;11. pii: 72313. doi:

Insert

hU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-CD90.1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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MAC_C_ROS1

Plasmid

#187766

Purpose

MAC-tagged gene expression of human ROS1

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

ROS1 (ROS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-eGFP-P2A-LgBiT-NLS

Plasmid

#187834

Purpose

Expression of eGFP-P2A-LgBiT-NLS from human synapsin promoter via adeno-associated virus for neuronal expression.

Depositor

William McEwan

Article

Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

Insert

eGFP-P2A-LgBiT-NLS

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 C-term SP100

Plasmid

#187584

Purpose

Express FLAG epitope and APEX2-tagged SP100 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

SP100 (SP100 Human)

Use

Tags

APEX2-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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