We narrowed to 12,360 results for: NUC

1. pENTR4-myc-nuc (w556-1)

   Plasmid

   #20026

   Purpose

   Gateway entry vector for nuclear targeting of domains (C-term NLS, myc tags)

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Gateway entry vector

   Tags

   Myc and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. nuclear EKAR (EGFP-mRFP)

   Plasmid

   #18682

   Depositor

   Karel Svoboda

   Article

   Harvey et al PNAS 2008 Dec 9;105(49):19264-9

   Insert

   ERK Activity Reporter
   

   Use

   Tags

   EGFP and mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOTTC293 - pAAV EF1a V5-synuclein (WT)

   Plasmid

   #60057

   Purpose

   An AAV packaging vector that expresses wildtype alpha-synuclein under control of the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

   Insert

   alpha-synuclein (SNCA Human, human)
   

   Use

   AAV

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. nuclear EKAR (Cerulean-Venus)

   Plasmid

   #18681

   Depositor

   Karel Svoboda

   Article

   Harvey et al PNAS 2008 Dec 9;105(49):19264-9

   Insert

   ERK Activity Reporter
   

   Use

   Tags

   monomeric Cerulean and monomeric Venus

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG426-GAL-Alpha-synuclein

   Plasmid

   #181715

   Purpose

   Galactose inducible expression of Alpha-synuclein

   Depositor

   Alejandro Chavez

   Insert

   Alpha-synuclein (SNCA Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pD454-SR rat Alpha-synuclein

   Plasmid

   #89074

   Purpose

   bacterial expression of rat Alpha-synuclein

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   Alpha-synuclein (Snca Rat)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 17, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pOpen-Exonuclease III E. coli

   Plasmid

   #165571

   Purpose

   Double-stranded DNA specific exonuclease. Initiates at the 3' termini of linear double-stranded DNA with 5' overhangs or blunt ends and 3' overhangs containing less than four bases. Initiates at nicked sites in double-stranded DNA.

   Depositor

   Jennifer Molloy, Drew Endy, FreeGenes Project

   Insert

   Exonuclease III E. coli
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCDNA3.0_nucLAMA-G97 IRES EGFP

   Plasmid

   #136620

   Purpose

   Plasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   SNAP-Tag-LAMA-G97-NLS (IRES EGFP)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. E407V Nuclear Myosin I YFP

   Plasmid

   #60242

   Purpose

   Expresses a E407V mutant of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding and motor activities

   Depositor

   Primal de Lanerolle

   Article

   Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

   Insert

   Nuclear Myosin 1 (Myo1c Mouse)
   

   Use

   Tags

   EYFP and NLS

   Expression

   Mammalian

   Mutation

   E407V

   Promoter

   Available since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3-Nuc-ExRai-AMPKAR

    Plasmid

    #192450

    Purpose

    ExRai-AMPKAR targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    ExRai-AMPKAR-NLS
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pD434-SR mouse Alpha-synuclein

    Plasmid

    #89073

    Purpose

    bacterial expression of mouse Alpha-synuclein

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    Alpha-synuclein (Snca Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 17, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pICH-nuc-roGFP2-synORP1

    Plasmid

    #191759

    Purpose

    Ti plasmid pICH86988 harbouring nuc-roGFP2-synORP1 for H2O2 detection in the nuclei of plant cells.

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    nuc-roGFP2-synORP1
    

    Use

    Binary agrobacterium ti plasmid

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. V5-BS2-SENP2 (Nuclear Pore)

    Plasmid

    #220345

    Purpose

    Mammalian expression vector BS2 in nuclear pore

    Depositor

    Bryan Dickinson

    Article

    Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

    Insert

    BS2
    

    Use

    Tags

    SENP2 (nuclear pore localized protein) and V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pET-29b(+)_NSP4ss_SerM_NucA-myc

    Plasmid

    #218191

    Purpose

    Bacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)

    Depositor

    Florence Pojer

    Insert

    nucA
    

    Use

    Tags

    NSP4 signal secretion peptide and myc

    Expression

    Bacterial

    Mutation

    codon optimized ecoli residues - 22-266

    Promoter

    T7

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICH-nuc-roGFP2-ORP1

    Plasmid

    #191758

    Purpose

    Ti plasmid pICH86988 harbouring nuc-roGFP2-ORP1 for peroxide detection in the nuclei of plant cells.

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    nuc-roGFP2-ORP1
    

    Use

    Binary agrobacterium ti plasmid

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Nuc-mTurq2-Apollo-NADP+

    Plasmid

    #193302

    Purpose

    Expresses mTurq2-Apollo-NADP+ sensor localized to the nucleus

    Depositor

    Jonathan Rocheleau

    Article

    Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

    Insert

    G6PD (G6PD Human)
    

    Use

    Tags

    SV40 NLS and mTurqoise2

    Expression

    Mammalian

    Mutation

    S40A, R72Q, H201N, K205T

    Promoter

    T7

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pICH-nuc-roGFP2-GPXC71S

    Plasmid

    #191760

    Purpose

    Ti plasmid pICH86988 harbouring nuc-roGFP2-GPXC71S -a nuclear-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191759 and 191758

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    nuc-roGFP2-GPXC71S
    

    Use

    Binary agrobacterium ti plasmid

    Tags

    Expression

    Plant

    Mutation

    GPX has no peroxidatic Cysteine 71, which is repl…

    Promoter

    CaMV 35S

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3.0_nucLAMA-F98 IRES EGFP

    Plasmid

    #136618

    Purpose

    Plasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    SNAP-Tag-LAMA-F98-NLS (IRES EGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV NucleoBAS N354K-I372V

    Plasmid

    #63574

    Purpose

    Fluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET

    Depositor

    Stan van de Graaf

    Article

    van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:

    Insert

    NucleoBAS N354K-I372V
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    N345K and I372V

    Promoter

    CMV

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pOpen-Endonuclease VIII E. coli

    Plasmid

    #165561

    Purpose

    Bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities; The N-glycosylase activity releases damaged pyrimidines, including thymine glycol and uracil glycol. The AP lyase activity cleaves DNA phosphodiester backbone at AP sites via beta and delta-elimination, creating a 1 nucleotide DNA gap with 5' and 3' phosphate termini.

    Depositor

    Jennifer Molloy, Drew Endy, FreeGenes Project

    Insert

    Endonuclease VIII E. coli
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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