We narrowed to 6,121 results for: cas9 expression plasmid
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Plasmid#104417Purposetransient expression of dCas9-dHDAC1 fusion proteinDepositorAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only
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FASTHDR-tRNApromo-sgRNA-espCas91_1
Plasmid#167210PurposePlasmid for easy cloning of a sgRNA sequence. The Plasmid contain a tRNA promoter to facilitate the expression of any sgRNA sequence and also expresses eSpCas9(1.1)DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterCMVAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYJ28-AAVS1-XLone-dCas9-p300core
Plasmid#131071PurposeDonor plasmid of targeted inducible-dCAS9-P300core knockinDepositorInsertdCas9-p300core
UseCRISPRExpressionMammalianAvailable SinceOct. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
dCas9v2
Plasmid#103140PurposeTetR inducible dCas9-VPR plasmid with pRPR-NotI-tRPR1 Csy4-ready siteDepositorInsertdCas9-VPR
ExpressionYeastMutationRemoved scaffold gRNA from original plasmidAvailable SinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-dTET1-H1672Y-D1674A-Hygro
Plasmid#104413Purposetransient expression of dCas9-dTET1 fusion proteinDepositorInsertdTET1
UseCRISPRExpressionMammalianMutationH1672Y-D1674APromoterCMV promoterAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-JMJD2A-S288G-T289G-Hygro
Plasmid#104418Purposetransient expression of dCas9-dJMJD2A fusion proteinDepositorInsertdJMJD2A
UseCRISPRExpressionMammalianMutationS288G-T289GPromoterCMV promoterAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEVA47_dCas9-GFP_NOT_iC
Plasmid#231418PurposeThis NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-C1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ290.)DepositorInsertsdCas9
GFP
UseSynthetic BiologyAvailable SinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSEVA47_dCas9-GFP_NOT_iA
Plasmid#231417PurposeThis NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-A1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ285.)DepositorInsertsdCas9
GFP
UseSynthetic BiologyAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-LSD1-K661A-Hygro
Plasmid#104414Purposetransient expression of dCas9-dLSD1 fusion proteinDepositorInsertdLSD1 (KDM1A Human)
UseCRISPRExpressionMammalianMutationIRATp970A0364D (Source Bioscience) has a P405H ch…PromoterCMV promoterAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-p300-S1396R-S1397R-Hygro
Plasmid#104419Purposetransient expression of dCas9-dp300 fusion proteinDepositorInsertdp300
UseCRISPRExpressionMammalianMutationS1396R-S1397RPromoterCMV promoterAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cas9_nonPuro_OSS
Plasmid#186652PurposeCas9 plasmid to express the Cas9 in OSS cellsDepositorInsertOSS Cas9 Expression Plasmid
ExpressionInsectAvailable SinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
FU_tetO_dCas9-VP192-T2A-EGFP
Plasmid#121173PurposeLentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.DepositorInsertdCas9-VP192-T2A-EGFP
UseCRISPR and Lentiviral; Inducible by doxycyclineTagsT2A-EGFP-ires-puroExpressionMammalianAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.3 CAS9 IRES-Dsred
Plasmid#129444Purposeexpression of FnCas9 and DsRedDepositorInsertFnCas9 IRES-Dsred
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pb-CAGGS-dCas9-blasto
Plasmid#218555PurposeEncodes CAG promoter-driven dCas9 with a C-terminal bipartite SV40 NLS, along with blasticidin resistanceDepositorInsertdCas9
UseCRISPR; Piggybac transpositionTags2xSV40NLSExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…Available SinceAug. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX458-HypaCas9
Plasmid#190604PurposePX458 (Plasmid #48138) with the N692A, M694A, Q695A, and H698A mutationsDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME-dCas9-BFP-KRAB
Plasmid#221502PurposeCRISPRi machinery fish codon optimizedDepositorInsertdCas9-tagBFP-KRAB
UseFish expressionMutationD10A and H839A point mutationsAvailable SinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro-V2.0-sgHsAGO3_AH
Plasmid#148855PurposeMammalian Expression of HsAGO3-sgRNADepositorAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pISV-CRISPR/Cas9PGmUbi
Plasmid#209189PurposeExpression of Cas9 in the model legume Medicago truncatula, Gmubi promoter (PGmubi), DsRed and Basta selectionDepositorInsertsCas9
DsRed(deltaB)
TagsNLSExpressionPlantMutationmissing BsaI site and plant-codon optimized with …PromoterCaMV 35S and GmUbi from Glycine max (L.) Merr. cv…Available SinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-sgRNA-RFP
Plasmid#166005PurposeBacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)DepositorTypeEmpty backboneUseCRISPRExpressionBacterialAvailable SinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330-U6-BRD4-HaloTag-Guide-hspCas9
Plasmid#228576PurposeExpression of Halo-tagged human BRD4 gRNA; CRISPR-mediated gene insertionDepositorAvailable SinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only