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pTHSSd_29

Plasmid

#59959

Purpose

PTac alpha fragment expression

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

alpha fragment

Expression

Bacterial

Mutation

N/A

Promoter

PTac

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pTH1-SaSrtA

Plasmid

#64973

Purpose

Expresses the wild type of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59

Promoter

T7 promoter

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pMB1-spect-PthrC3_8-eGFP

Plasmid

#107411

Purpose

This plasmid contain promoter PthrC3_8, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

Depositor

Kang Zhou

Article

Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

Insert

eGFP

Expression

Bacterial

Promoter

PthrC3_8

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pTH727-CEN-RLuc/staCFLuc

Plasmid

#38211

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

The last three codons of the full-length Firefly …

Promoter

ADH1 and TDH3 (=GPD)

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pRSET FlipSuc-90u delta1

Plasmid

#14475

Depositor

Wolf Frommer

Article

Lager et al J Biol Chem. 2006 Oct 13. 281(41):30875-

Insert

FlipThuE W283A

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

N192D, W283A, 13 amino acids deleted from linkers

Promoter

T7

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pTH847-gst-optsc

Plasmid

#96908

Purpose

Plasmid for yeast expression of GST encoded by preferred codons

Depositor

Tobias von der Haar

Article

Jossé et al bioRxiv 200006

Insert

yeast codon optimised glutathione-S transferase

Tags

8xhistidine

Expression

Yeast

Promoter

TDH3

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pTH652-RLuc/maxFLuc

Plasmid

#29698

Depositor

Tobias von der Haar

Article

Chu et al Nucleic Acids Res. 2011 Aug;39(15):6705-

Insert

Firefly Luciferase

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pBS-PtH2B

Plasmid

#133390

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Expression

Mammalian

Promoter

No promoter

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pTHsp18.2 (GB0035)

Plasmid

#68186

Purpose

Provides the Arabidopsis thaliana heat shock protein 18.2 terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtHsp18.2 terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pTH1-SaSrtA-KA

Plasmid

#64981

Purpose

Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K, E108A

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pTH646-2µ-RLuc

Plasmid

#40600

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

Renilla Luciferase

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pTH385-GST-TEV-SUP35

Plasmid

#29744

Depositor

Tobias von der Haar

Insert

SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

wild type

Available Since

Aug. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pTH732-2µ-RLuc/staCFLuc

Plasmid

#40607

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

The last three codons of the full-length Firefly …

Promoter

ADH1 and TDH3

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pTHMT_hnRNPF_deltaPLD

Plasmid

#139115

Purpose

express FL hnRNPF without the prion like domain with N-terminal maltose binding protein tag

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPF (HNRNPF Human)

Expression

Bacterial

Mutation

delta365-415

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pETM6_Ptrc_BsMdh_BmHps_BmPhi_Pthl_CaThl_CaCtfAB_CaAdc

Plasmid

#129105

Purpose

Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus as well as Clostridium acetobutylicum genes required for Acetate-dependent Acetone production

Depositor

Eleftherios Terry Papoutsakis

Article

Bennett et al Metab Eng. 2018 Jan;45:75-85. doi: 10.10

Insert

BsMDH BmHPS BmPHI CaTHL CaCtfAB CaADC

Expression

Bacterial

Available Since

Nov. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pTHSSe_32

Plasmid

#109236

Purpose

PUPsp2 GFP reporter

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PUPsp2

Available Since

July 23, 2018

Availability

Academic Institutions and Nonprofits only

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pThom77.2

Plasmid

#101646

Purpose

Encodes the fluorescent protein amFP486 for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

amFP486

Tags

6XHis

Expression

Bacterial

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pTH09

Plasmid

#70064

Purpose

artificial transcription template "terminator insertion"

Depositor

Jeff Gelles

Article

Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

Insert

lambda PR' - lambda TR2 - repeat cassette - E. coli rpoB - lambda TR'

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only
pTHSSd_39

Plasmid

#59962

Purpose

Low core fragment expression (low resource allocator)

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

core fragment

Expression

Bacterial

Mutation

N/A

Promoter

PJ23105

Available Since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pTHSSd_40

Plasmid

#59963

Purpose

High core fragment expression without SynZIP

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

core fragment lacking SynZIP

Expression

Bacterial

Mutation

N/A

Promoter

PJ23105

Available Since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only

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