Skip to main content
Addgene
Showing: 121 - 140 of 3333 results

  7. pUPD2 PhiC31 RL (attR:P35S:TMtb:attL) (GB1506)

    Plasmid
    #160582
    Purpose
    35S promoter inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attR and attL)
    Depositor
    Diego Orzaez
    Article
    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379
    Insert
    attR:P35S:TMtb:attL
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160582-plasmid-map-sequence-id-322083

    Enlarge

  9. pX458-Dual-Guide-Donor-ShuttleOnly

    Plasmid
    #171098
    Purpose
    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.
    Depositor
    Joseph Brzezinski
    Article
    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    XbaI site added upstream of U6 promotor. GtoC mu…
    Promoter
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    171098-plasmid-map-sequence-id-336112

    Enlarge

  13. pShuttle-CMV-F2A-T2A-Venus

    Plasmid
    #62621
    Purpose
    AdEasy Shuttle vector modified for multicistronic expression. Contains T2A, F2A and Venus tag
    Depositor
    Zheng-Xu Wang
    Insert
    MCS with F2A and T2A
    Use
    Adenoviral
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62621-plasmid-map-sequence-id-102957

    Enlarge

  19. pUCIDT-attL1-Worm ABeta-attR5

    Plasmid
    #160437
    Purpose
    Entry vector for cloning nematode-codon-optimized Amyloid Beta(1-42) using 2-fragment gateway recombination
    Depositor
    Nicholas Tolwinski
    Article
    Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi
    Insert
    Homo sapiens amyloid beta precursor protein (APP Nematode, Human)
    Use
    Tags
    Cleavage signal
    Expression
    Bacterial
    Mutation
    Codon-optimized for expression in C. elegans
    Promoter
    Available since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    160437-plasmid-map-sequence-id-314980

    Enlarge

  20. pLenti-C-Myc-DDK-IRES-Puro METTL3

    Plasmid
    #200724
    Purpose
    Lentiviral expression of Flag-Tagged human METTL3
    Depositor
    Claudio Alarcón
    Insert
    METTL3 (METTL3 Human)
    Use
    Lentiviral
    Tags
    Myc-DDK from backbone plasmid
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    200724-plasmid-map-sequence-id-401795

    Enlarge

Showing: 121 - 140 of 3333 results