Addgene

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pBABEpuro-Flag-ZO-2

Plasmid

#27420

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
pLib6.4-Cquin_596

Plasmid

#176655

Purpose

Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039596) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pSL1180-HR-PUbEGFP-NLS

Plasmid

#176681

Purpose

Expression of nuclear localized EGFP under Ae. aegypti Polyubiquitin promoter (AAEL003888), EGFP version of pSL1180-HR-PUbECFP (Addgene #47917)

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Ae. aegypti poly-ubiquitin (AAEL003888)

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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KHBD00311

Plasmid

#34436

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG9786 (hb Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00308

Plasmid

#34433

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG5204 (CG5204 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf137

Plasmid

#12825

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf137

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
KHBD00322

Plasmid

#34447

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8591 (CTCF Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pJMP1102

Plasmid

#119246

Purpose

knockdown phzA1 in P. aeruginosa

Depositor

Jason Peters, Carol Gross, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA phzA1 (P. aeruginosa)

Use

Tags

Expression

Bacterial

Mutation

D10A and H840A

Promoter

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1103

Plasmid

#119247

Purpose

knockdown phzM in P. aeruginosa

Depositor

Jason Peters, Carol Gross, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA phzM (P. aeruginosa)

Use

Tags

Expression

Bacterial

Mutation

D10A and H840A

Promoter

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG HDAC5 S259A

Plasmid

#32214

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

HDAC5 (HDAC5 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Ser259Ala

Promoter

CMV

Available since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-myc-Sema4A

Plasmid

#115171

Purpose

Expresses membrane-targeted Sema4A with N-terminal myc tag

Depositor

Suzanne Paradis

Article

McDermott et al Mol Cell Neurosci. 2018 Jul 4;92:50-66.

Insert

Sema4A (Sema4a Mouse)

Use

Tags

membrane targeting sequence and myc

Expression

Mammalian

Mutation

First 32 amino acids of Sema4A replaced with memb…

Promoter

CMV

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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KHBD00433

Plasmid

#34538

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG9745 (D1 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00606

Plasmid

#39684

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG15715 (CG15715 Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
pEJS481-pCDest2-AcrE2-mTagBFP2-IRES

Plasmid

#85749

Purpose

Expresses anti-CRISPR protein from Type I-E and mTagBFP2

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Inserts

Type I-E anti-CRISPR
mTagBFP2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pJMP1239

Plasmid

#119263

Purpose

knockdown folA in P. aeruginosa

Depositor

Jason Peters, Carol Gross, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA folA (P. aeruginosa)

Use

Tags

Expression

Bacterial

Mutation

D10A and H840A

Promoter

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA pDEST40 Cas9 GW STOP

Plasmid

#183196

Purpose

Destination vector

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221 Cas9-HMGB1

Plasmid

#183198

Purpose

Entry clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

Cas9-HMGB1

Use

CRISPR; Gateway entry clone

Tags

Expression

Mutation

n/a

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221 Cas9-RFC4

Plasmid

#183199

Purpose

Entry clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

Cas9-RFC4

Use

CRISPR; Gateway entry clone

Tags

Expression

Mutation

n/a

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLib6.4B-Aaeg_774

Plasmid

#176673

Purpose

Expression of sgRNA under Ae. Aegypti U6 (AAEL017774) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLib6.4B-Cquin_596

Plasmid

#176669

Purpose

Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039596) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge