Addgene

We narrowed to 23,409 results for: Spr;

Showing: 1381 - 1400 of 23409 results

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pSET-dCas9-actII-4-NT-S1

Plasmid

#110185

Purpose

Repression of gene expression in Streptomyces by CRISPRi

Depositor

Yinhua Lu

Article

Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

Inserts

dCas9
sgRNA

Use

CRISPR; I

Tags

Expression

Bacterial

Mutation

D10A, H840A

Promoter

J23119 and ermE*p

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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AcrIIA4

Plasmid

#101043

Purpose

Anti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.

Depositor

Zhiwei Huang

Article

Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

Insert

AcrIIA4

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pHdzCas9-KRAB

Plasmid

#92341

Purpose

Expression dzCas9-KRAB in microalgae. CRISPR/Cas based plant genome editing and gene regulation, Hyg resistance

Depositor

I-Son Ng

Article

Kao et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Use

CRISPR; Plant expression

Tags

KRAB

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pSBY1_FnCpf1cg

Plasmid

#104622

Purpose

CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn CPf1

Depositor

Sheng Yang

Article

Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

Insert

FnCpf1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

codon-optimized C.glutamicum

Promoter

hsp60

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pYZ301

Plasmid

#102690

Purpose

S. pombe gRNA entry vector with Cas9 - Sp lys9 marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pRPaCas9

Plasmid

#111819

Purpose

Expresses Cas9 in T. brucei (2T1) cells

Depositor

David Horn

Article

Rico et al Sci Rep. 2018 May 21;8(1):7960. doi: 10.

Insert

Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pYZ294

Plasmid

#102688

Purpose

S. pombe gRNA entry vector with Cas9 - KanMX marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ300

Plasmid

#102689

Purpose

S. pombe gRNA entry vector with Cas9 - Sp his3 marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ293

Plasmid

#102687

Purpose

S. pombe gRNA entry vector with Cas9 - Sp leu1 marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pspCas9-3'UTR-ST2-com-vector

Plasmid

#136269

Purpose

Expresses SpCas9 and sgRNA Scaffold

Depositor

Baisong Lu

Article

Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

Insert

SpCas9 and sgRNA scaffold

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA1-boxB-MS2-PP7

Plasmid

#75397

Purpose

sgRNA1-boxB-MS2-PP7

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-boxB-MS2-PP7

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-a5 guide B

Plasmid

#194011

Purpose

For CRISPR knockout of integrin alpha5

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (a5_guide B)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-aV guide A

Plasmid

#194008

Purpose

For CRISPR knockout of integrin alphaV

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (aV_guide A)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-aV guide B

Plasmid

#194009

Purpose

For CRISPR knockout of integrin alphaV

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (aV_guide B)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-puro-a5 guide A

Plasmid

#194010

Purpose

For CRISPR knockout of integrin alpha5

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

single-guide RNA (a5_guide A)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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JME4390

Plasmid

#129656

Purpose

LEU2ex_CrisprCas9-yl_RFP: CAS9 vector with LEU2ex marker for gRNA cloning using GoldenGate

Depositor

Tristan Rossignol

Article

Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL081

Plasmid

#137890

Purpose

iSTU-CRISPR/Cas9 with OsCDPK2 intron, tRNA structure backbone

Depositor

Yong Zhang

Article

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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SP6-sgRNA-scaffold

Plasmid

#47912

Purpose

Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection

Depositor

Paul Sternberg

Article

Chiu et al Genetics. 2013 Aug 26.

Type

Empty backbone

Use

CRISPR

Tags

sgRNA 3' end

Expression

Worm

Mutation

Promoter

SP6

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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SpyCas9(WT)

Plasmid

#101044

Purpose

Express Streptococcus pyogenes Cas9

Depositor

Zhiwei Huang

Article

Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

Insert

SpyCas9

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pSDMA65

Plasmid

#128354

Purpose

Codon optimised Streptococcus pyogenes CAS9 ORF regulated by the Cryptococcus neoformans TEF1 promoter and terminator.

Depositor

James Fraser

Article

Arras et al PLoS One. 2016 Oct 6;11(10):e0164322. do

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

ACT1

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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