We narrowed to 59,686 results for: plasmid
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Plasmid#124229PurposeTargets the human CLYBL safe harbor locus for integration of dox-inducible NGN2 for differentiation of iPSCs into cortical neurons. Contains a floxed BSD-NLS-mApple selection cassette.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNGN2 (NEUROG2 Human)
ExpressionMammalianAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCS2-Cas9
Plasmid#47322PurposeFor in vivo expression of Cas9 or generation of Cas9 mRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9
UseCRISPR; Xenopus and zebrafish expressionExpressionMammalianPromoterSP6Available sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZac2.1-GfaABC1D-mCherry-hPMCA2w/b
Plasmid#111568PurposeExpresses a plasma membrane calcium pump hPMCA2w/bDepositorHas serviceAAV5InsertPlasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
UseAAVTagsmCherryAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Human IFN-gamma promoter/enhancer
Plasmid#17598DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertIFN gamma promoter with 1st intron enhancer (IFNG Human)
TagsluciferaseExpressionMammalianMutation-3.6 kb to +70 of promoter plus 680 bp of IFN-gam…Available sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-PE7-P2A-GFP
Plasmid#222996PurposeMammalian expression of SpCas9 PE7 prime editor with P2A-EGFP markerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPE7-P2A-EGFP
TagsP2A-EGFP, SV40 bpNLS, and c-Myc NLSExpressionMammalianPromoterCMVAvailable sinceAug. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLEX-VSV-G-Foldon-Intein-Cas9
Plasmid#247731PurposeExpresses VSV-G, Foldon and Cas9, separated by self-cleaving inteinDepositorInsertVSV-G-Foldon-Intein-Cas9
UseCRISPRExpressionMammalianPromoterCAGAvailable sinceAug. 19, 2026AvailabilityAcademic Institutions and Nonprofits only -
His6-TEV-CRBN-midi
Plasmid#215330PurposeCRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader designDepositorInsertCRBN (CRBN Human)
Tags6xHis and TEVExpressionBacterialMutationcontains amino acids 41-187 followed by a Gly-Ser…PromoterlacIAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hsyn-GRAB_5-HT1.0
Plasmid#140552PurposeExpresses the 5-HT sensor GRAB_5-HT1.0 in neuronsDepositorHas serviceAAV9Insert5-HT sensor GRAB_5-HT1.0
UseAAVPromoterhSynAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSynSRE-T-Luc
Plasmid#60444PurposeUses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHMG-CoA synthase promoter (-324/-225)
UseLuciferaseTagsLuciferaseExpressionMammalianPromoterHMG-CoA synthase (-324/-225) & TATAA (-28/+39)Available sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)
Plasmid#199604PurposeExpression of cell permeable spCas9 in E. Coli.DepositorInsertCas9-T6N
UseCRISPRExpressionBacterialAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]
Plasmid#62723PurposeAAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.DepositorHas serviceAAV1 and AAV5InsertChrimsonR-tdTomato
UseAAVTagstdTomatoExpressionMammalianMutationChrimson K176R mutantPromoterhuman synapsin promoterAvailable sinceApril 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.CAG.GCaMP6f.WPRE.SV40
Plasmid#100836PurposeAAV expression of ultrasensitive protein calcium sensor from CAG promoterDepositorHas serviceAAV1, AAV2, and AAV9InsertGCaMP6f
UseAAVExpressionMammalianMutationGCaMP3-T302L R303P A317E D380Y T381R S383T R392GPromoterCAGAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-GRAB_NE1m
Plasmid#123308PurposeExpresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neuronsDepositorHas serviceAAV9 and AAV RetrogradeInsertGPCR activation based NE sensor GRAB_NE1m
UseAAVMutationcontains a glycine-to-threonine mutation at posit…PromoterhSynAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-mDlx-ChR2-mCherry-Fishell-3
Plasmid#83898PurposeChR2-mCherry fusion expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer elementDepositorHas serviceAAV1, AAV9, and AAV RetrogradeInsertChR2
UseAAVExpressionMammalianPromotermDlxAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hsyn-GRAB_DA2m
Plasmid#140553PurposeExpresses the 2nd generation medium-affinity DA sensor GRAB_DA2m in neuronsDepositorHas serviceAAV9InsertThe 2nd generation DA sensor GRAB_DA2m (medium affinity)
UseAAVPromoterhSynAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330a dCas9-LSD1
Plasmid#92362PurposeModified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to LSD1. For targeted enhancer demethylation in chicken embryos.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-LSD1
UseCRISPRExpressionMammalianPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE
Plasmid#104495PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switchDepositorHas serviceAAV1, AAV PHP.eB, and AAV RetrogradeInsertjGCaMP7s
UseAAV and Cre/LoxTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianPromoterCAGAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pY094
Plasmid#84743PurposeExpresses huAsCpf1-T2A-GFP and crRNA guideDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertshuAsCpf1
GFP
Tags3xHA, NLS, and T2AExpressionMammalianPromoterCMVAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL3-Sox2
Plasmid#101761PurposeSox2 promoter reporterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSRY-box 2 promoter (SOX2 Human)
UseLuciferaseExpressionMammalianAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBA904
Plasmid#122238PurposeLentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.DepositorInsertsgRNA with cs1 in stem loop 2 of the constant region
UseCRISPR and LentiviralExpressionMammalianAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by