8,808 results

1. ppyCAG_RNaseH1_D210N

   Plasmid

   #111904

   Purpose

   Express D210N mutated RNASEH1 which abolishes the catalytic activity of RNASEH1

   Depositor

   Xiang-Dong Fu

   Article

   Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

   Insert

   RNASEH1 (RNASEH1 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Changed D 210 to N

   Available Since

   July 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-CMV-Blast-PIP-FUCCI

   Plasmid

   #138715

   Purpose

   Lentiviral expression of the cell cycle marker PIP-FUCCI

   Depositor

   Jean Cook

   Article

   Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

   Insert

   PIP-FUCCI
   

   Use

   Lentiviral

   Tags

   mCherry and mVenus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1a-FLEX-GTB

   Plasmid

   #26197

   Depositor

   Edward Callaway

   Article

   Haubensak et al Nature. 2010 Nov 11. 468(7321):270-6.

   Insert

   Enhanced green fluorescent protein, TVA, rabies B19 glycoprotein
   

   Use

   AAV and Cre/Lox; Adeno-associated virus

   Mutation

   TVA, rabies glycoprotein are mammalian codon opti…

   Available Since

   Nov. 11, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. Laconic/pcDNA3.1(-)

   Plasmid

   #44238

   Purpose

   a genetically-encoded sensor for lactate concentration, transport and metabolic flux

   Depositor

   Luis Felipe Barros

   Article

   San Martin et al PLoS One. 2013;8(2):e57712. doi: 10.1371

   Insert

   Laconic (LACtate Optical Nano Indicator from CECs)
   

   Use

   Lactate biosensor

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVUT-tTR-KRAB

   Plasmid

   #11651

   Purpose

   Tet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 3rd generation

   Depositor

   Didier Trono, Patrick Aebischer

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Has Service

   Cloning Grade DNA

   Insert

   hUbiquitin C, GFP, tTR-KRAB, Tet-on
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.CMV.PI.EGFP.WPRE.bGH

   Plasmid

   #105530

   Purpose

   AAV expression of EGFP from CMV promoter

   Depositor

   James M. Wilson

   Has Service

   AAV PHP.eB, AAV rh10, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   EGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKDsgRNA-ack

   Plasmid

   #62654

   Purpose

   Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression

   Depositor

   Kristala Prather

   Article

   Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

   Insert

   exo, beta, gam, sgRNA
   

   Expression

   Bacterial

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS

   Plasmid

   #60904

   Purpose

   The plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   scFv-GCN4
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. Str-Ii_VSVG-SBP-mCherry

   Plasmid

   #65301

   Purpose

   synchronize trafficking of VSVG from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   VSV-G
   

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-2xFYVE

    Plasmid

    #140047

    Purpose

    Expresses GFP-tagged HRS 2xFYVE in mammalian cells

    Depositor

    Harald Stenmark

    Article

    Gillooly et al EMBO J. 2000 Sep 1;19(17):4577-88. doi:

    Insert

    tandem HRS FYVE domain (Hgs Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    two FYVE domains (amino acids 147-223), linked by…

    Promoter

    CMV

    Available Since

    May 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3XFlagNICD2

    Plasmid

    #20184

    Depositor

    Raphael Kopan

    Article

    Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

    Insert

    Notch 2 intracellular domain (Notch2 Mouse)
    

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Contains murine Notch 2 Intracellular Domain (Met…

    Promoter

    CMV

    Available Since

    Jan. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. SpyCatcher003

    Plasmid

    #133447

    Purpose

    Expresses SpyCatcher003 in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyCatcher003
    

    Tags

    GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. v3em-Cterm-PE2max-∆RNaseH-dualU6

    Plasmid

    #198735

    Purpose

    AAV genome encoding C-terminal PE2max and U6 expression cassettes

    Depositor

    David Liu

    Article

    Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

    Insert

    NpuC-CtermPE2max∆RNaseH
    

    Use

    AAV

    Promoter

    Cbh

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Flag-hPIASx beta

    Plasmid

    #15210

    Depositor

    Ke Shuai

    Article

    Arora et al J Biol Chem. 2003 Jun 13. 278(24):21327-

    Insert

    PIASx beta (PIAS2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. CARPID BASU-dCasRx

    Plasmid

    #153209

    Purpose

    express CARPID BASU-dCasRx fusion protein in mammalian cells

    Depositor

    Jian Yan, Liang Zhang

    Article

    Yi et al Nat Methods. 2020 Jun 22. pii: 10.1038/s

    Insert

    BASU-dCasRx
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    R239A/H244A/R858A/H863A in RfxCas13d

    Promoter

    EF-1a

    Available Since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQE30-His-CKM

    Plasmid

    #124134

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Creatine kinase (CKM Chicken)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-E-cadherin-RFP

    Plasmid

    #79603

    Purpose

    pHAGE lentiviral vector with dsRed driven by the human E-cadherin promoter. When used in combination with FUGW-d2GFP-ZEB1 3' UTR, comprises the Z-cad dual sensor. Use 2nd generation packaging system

    Depositor

    Jeffrey Rosen

    Article

    Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

    Insert

    CDH1 promoter region (CDH1 Human)
    

    Use

    Lentiviral

    Available Since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-hPIASy

    Plasmid

    #15208

    Depositor

    Ke Shuai

    Article

    Liu et al Proc Natl Acad Sci U S A. 2001 Mar 13. 9

    Insert

    PIASy (PIAS4 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-ChrimsonR-tdT

    Plasmid

    #59169

    Purpose

    Expression vector for ChrimsonR-tdTomato

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-tdTomato
    

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    K176R

    Promoter

    CAG

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3-HA-Akt1

    Plasmid

    #73408

    Purpose

    Expresses human AKT1 with a N-terminus HA tag

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    AKT1 (AKT1 Human)
    

    Tags

    HA tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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